Q2YQV5 (SYFA_BRUA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha chain
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha chain
Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	BAB1_2126

Organism

Brucella abortus (strain 2308) [Complete proteome] [HAMAP]

Taxonomic identifier

359391 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00281
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm HAMAP MF_00281.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 369	369	Phenylalanine--tRNA ligase alpha chain HAMAP MF_00281		PRO_0000231968
Sites
	Metal binding	269	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2YQV5 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: BC2B822DB0270926	FASTA	369	41,598
	10 20 30 40 50 60 MSDLEQLERQ ILEDIAAAVD EQGIEAVRVA ALGKKGTVSE KLKTLGGMSP EERQMQGPAI 70 80 90 100 110 120 NGLKNRVTEA LSERRTELRK AAVAARLERE KVDVTLPVRE SAASRGRIHP ISQVIDEITA 130 140 150 160 170 180 IFADMGFSIA EGPDIETDYY NFTALNFPEG HPAREMHDTF FFNPDEKGER KLLRTHTSPV 190 200 210 220 230 240 QVHTMEKFAA MRDKEGRDEP IRIVIPGKTY RMDSDATHSP MFHQVEGLVV DKSANVANMK 250 260 270 280 290 300 WVLEEFCKAF FEVPSVKMRM RPSFFPFTEP SVEVDIQCDR SGPHVKFGEG NDWLEILGCG 310 320 330 340 350 360 MVHPNVLRMS GYDPEVYQGF AWGMGIDRIA MLKYGMPDLR AFFDADVRWI EHYGFRPLDI PTLFGGLSA

References

[1]

"Whole-genome analyses of speciation events in pathogenic Brucellae." Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E. Infect. Immun. 73:8353-8361(2005) [PubMed: 16299333] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 2308.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM040264 Genomic DNA. Translation: CAJ12082.1.
RefSeq	YP_415447.1. NC_007618.1.
3D structure databases
ProteinModelPortal	Q2YQV5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2YQV5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3788851.
GenomeReviews	Gene locus BAB1_2126 in contig AM040264_GR.
KEGG	bmf:BAB1_2126.
PATRIC	17847351. VBIBruMel86222_2211.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0016.
HOGENOM	HBG284353.
OMA	FRASYFP.
ProtClustDB	PRK00488.
Enzyme and pathway databases
BioCyc	BMEL359391:BAB1_2126-MONOMER.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR022911. Phe_tRNA_synth_alpha1_bac. IPR002319. Phenylalanyl-tRNA_Synthase. IPR010978. tRNA-bd_arm. [Graphical view]
KO	K01889.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00468. PheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_BRUA2

Accession

Primary (citable) accession number: Q2YQV5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	February 7, 2006
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program