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Protein

Phenylalanine--tRNA ligase beta subunit

Gene

pheT

Organism
Brucella abortus (strain 2308)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per tetramer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi454 – 4541MagnesiumUniRule annotation
Metal bindingi460 – 4601Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi463 – 4631MagnesiumUniRule annotation
Metal bindingi464 – 4641MagnesiumUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. phenylalanine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. phenylalanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
  2. tRNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Enzyme and pathway databases

BioCyciBABO359391:GKDV-2181-MONOMER.
BMEL359391:GJOQ-2181-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylalanine--tRNA ligase beta subunitUniRule annotation (EC:6.1.1.20UniRule annotation)
Alternative name(s):
Phenylalanyl-tRNA synthetase beta subunitUniRule annotation
Short name:
PheRSUniRule annotation
Gene namesi
Name:pheTUniRule annotation
Ordered Locus Names:BAB1_2127
OrganismiBrucella abortus (strain 2308)
Taxonomic identifieri359391 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000002719 Componenti: Chromosome I

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 804804Phenylalanine--tRNA ligase beta subunitPRO_0000232048Add
BLAST

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.UniRule annotation

Protein-protein interaction databases

STRINGi359391.BAB1_2127.

Structurei

3D structure databases

ProteinModelPortaliQ2YQV4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini38 – 148111tRNA-bindingUniRule annotationAdd
BLAST
Domaini401 – 47676B5UniRule annotationAdd
BLAST
Domaini710 – 80394FDX-ACBUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily.UniRule annotation
Contains 1 B5 domain.UniRule annotation
Contains 1 FDX-ACB domain.UniRule annotation
Contains 1 tRNA-binding domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0073.
HOGENOMiHOG000292085.
KOiK01890.
OMAiIGWIGKV.
OrthoDBiEOG6CCH1J.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.56.20. 1 hit.
3.30.70.380. 1 hit.
3.50.40.10. 1 hit.
HAMAPiMF_00283. Phe_tRNA_synth_beta1.
InterProiIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR012340. NA-bd_OB-fold.
IPR004532. Phe-tRNA-ligase_IIc_bsu.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR005121. PheS_beta_Fdx_antiC-bd.
IPR002547. tRNA-bd_dom.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
PfamiPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
PF03147. FDX-ACB. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
SMARTiSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
SM00896. FDX-ACB. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54991. SSF54991. 1 hit.
SSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR00472. pheT_bact. 1 hit.
PROSITEiPS51483. B5. 1 hit.
PS51447. FDX_ACB. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2YQV4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFTLSWLKD HLETDATLDE IVEKLTDIGL EVESVDDRAA FRAFTIARVL
60 70 80 90 100
TATRHPDADK LQVLSVDTGD GKPVQVVCGA PNARAGLVGV LGRPGDYVPG
110 120 130 140 150
LDVTLSVGKI RGVESFGMMC SERELELSDE HNGIIDLAEN APVGTSFAAY
160 170 180 190 200
MGLDDPIIEI GLTPNRADCT GIRGIARDLA AAGLGTLKNT LPDAVKGEGE
210 220 230 240 250
TPVKVILDQD AGNPFCTGFA LRMVKGVKNG PSPKWMQQRL KAIGLRPINA
260 270 280 290 300
LVDITNYVTF DQGRPLHVFD AAKVKGNLTV RTARDGETIL ALDQREYKLN
310 320 330 340 350
AGMYVIADEN GPESIAGIMG GEHSGCDENT VDVLIESALW DPRMIASTGR
360 370 380 390 400
ELGIVTDARY RFERGVDPEM MVPGAEIATK LVLELCGGQP TVLDVVGYKP
410 420 430 440 450
HTARVIDFPV TEVKRLTGLD VSYEDAFDIL KRLGFGVEGD GKTIRATVPS
460 470 480 490 500
WRGDVEGKAD LVEEVMRIHG INRIDPQPLP SHGAVNGRIL TTLQIRTRHA
510 520 530 540 550
RRMLASRGMM EAVTYSFISE AQAKAFGGGK PELKLANPIA ADMSDMRPSL
560 570 580 590 600
LPGLLAAAQR NADRGFDDIA LFEVSGIYEG DTPDKQRRVA GGVRRGTAKV
610 620 630 640 650
EGAGRFWAGN AAPVGVFDAK ADALAALEAA GAPVDRIQIE AGGPEWLHPG
660 670 680 690 700
RSGTLKLGPK VVLGTFGEFH PDTLEALDVS GALCGFEVYL DAIPEPKAKS
710 720 730 740 750
ARTKPALSLS LFQSLKRDYA FVVDAAVEAG NVVKAVSSAD KKLIVGVQVF
760 770 780 790 800
DIFTGASLGE GKKSIAVEVL LQPQDRTLTD EDLEALSKQI VASVAKQTGG

VLRG
Length:804
Mass (Da):85,838
Last modified:February 7, 2006 - v1
Checksum:iEABC1EC1A8230AA8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM040264 Genomic DNA. Translation: CAJ12083.1.
RefSeqiYP_415448.1. NC_007618.1.

Genome annotation databases

EnsemblBacteriaiCAJ12083; CAJ12083; BAB1_2127.
KEGGibmf:BAB1_2127.
PATRICi17847353. VBIBruMel86222_2212.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM040264 Genomic DNA. Translation: CAJ12083.1.
RefSeqiYP_415448.1. NC_007618.1.

3D structure databases

ProteinModelPortaliQ2YQV4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi359391.BAB1_2127.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ12083; CAJ12083; BAB1_2127.
KEGGibmf:BAB1_2127.
PATRICi17847353. VBIBruMel86222_2212.

Phylogenomic databases

eggNOGiCOG0073.
HOGENOMiHOG000292085.
KOiK01890.
OMAiIGWIGKV.
OrthoDBiEOG6CCH1J.

Enzyme and pathway databases

BioCyciBABO359391:GKDV-2181-MONOMER.
BMEL359391:GJOQ-2181-MONOMER.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.56.20. 1 hit.
3.30.70.380. 1 hit.
3.50.40.10. 1 hit.
HAMAPiMF_00283. Phe_tRNA_synth_beta1.
InterProiIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR012340. NA-bd_OB-fold.
IPR004532. Phe-tRNA-ligase_IIc_bsu.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR005121. PheS_beta_Fdx_antiC-bd.
IPR002547. tRNA-bd_dom.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
PfamiPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
PF03147. FDX-ACB. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
SMARTiSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
SM00896. FDX-ACB. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54991. SSF54991. 1 hit.
SSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR00472. pheT_bact. 1 hit.
PROSITEiPS51483. B5. 1 hit.
PS51447. FDX_ACB. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 2308.

Entry informationi

Entry nameiSYFB_BRUA2
AccessioniPrimary (citable) accession number: Q2YQV4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: February 7, 2006
Last modified: April 1, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Brucella abortus strain 2308
    Brucella abortus (strain 2308): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.