Phenylalanine--tRNA ligase beta subunit - Brucella abortus (strain 2308)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase beta subunit
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase beta subunit
Short name=PheRS

Gene names

Name:	pheT
Ordered Locus Names:	BAB1_2127

Organism

Brucella abortus (strain 2308) [Complete proteome] [HAMAP]

Taxonomic identifier

359391 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella ›

Protein attributes

Sequence length	804 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP-Rule MF_00283
Cofactor	Binds 2 magnesium ions per tetramer By similarity.
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily. Contains 1 B5 domain. Contains 1 FDX-ACB domain. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP tRNA processing Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 804

804

Phenylalanine--tRNA ligase beta subunit HAMAP-Rule MF_00283

PRO_0000232048

Regions

Domain

38 – 148

111

tRNA-binding

Domain

401 – 476

76

B5

Domain

710 – 803

94

FDX-ACB

Sites

Metal binding

454

1

Magnesium By similarity

Metal binding

460

1

Magnesium; via carbonyl oxygen By similarity

Metal binding

463

1

Magnesium By similarity

Metal binding

464

1

Magnesium By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q2YQV4 [UniParc].

Last modified February 7, 2006. Version 1.
Checksum: EABC1EC1A8230AA8
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FASTA

804

85,838

        10         20         30         40         50         60 
MKFTLSWLKD HLETDATLDE IVEKLTDIGL EVESVDDRAA FRAFTIARVL TATRHPDADK 

        70         80         90        100        110        120 
LQVLSVDTGD GKPVQVVCGA PNARAGLVGV LGRPGDYVPG LDVTLSVGKI RGVESFGMMC 

       130        140        150        160        170        180 
SERELELSDE HNGIIDLAEN APVGTSFAAY MGLDDPIIEI GLTPNRADCT GIRGIARDLA 

       190        200        210        220        230        240 
AAGLGTLKNT LPDAVKGEGE TPVKVILDQD AGNPFCTGFA LRMVKGVKNG PSPKWMQQRL 

       250        260        270        280        290        300 
KAIGLRPINA LVDITNYVTF DQGRPLHVFD AAKVKGNLTV RTARDGETIL ALDQREYKLN 

       310        320        330        340        350        360 
AGMYVIADEN GPESIAGIMG GEHSGCDENT VDVLIESALW DPRMIASTGR ELGIVTDARY 

       370        380        390        400        410        420 
RFERGVDPEM MVPGAEIATK LVLELCGGQP TVLDVVGYKP HTARVIDFPV TEVKRLTGLD 

       430        440        450        460        470        480 
VSYEDAFDIL KRLGFGVEGD GKTIRATVPS WRGDVEGKAD LVEEVMRIHG INRIDPQPLP 

       490        500        510        520        530        540 
SHGAVNGRIL TTLQIRTRHA RRMLASRGMM EAVTYSFISE AQAKAFGGGK PELKLANPIA 

       550        560        570        580        590        600 
ADMSDMRPSL LPGLLAAAQR NADRGFDDIA LFEVSGIYEG DTPDKQRRVA GGVRRGTAKV 

       610        620        630        640        650        660 
EGAGRFWAGN AAPVGVFDAK ADALAALEAA GAPVDRIQIE AGGPEWLHPG RSGTLKLGPK 

       670        680        690        700        710        720 
VVLGTFGEFH PDTLEALDVS GALCGFEVYL DAIPEPKAKS ARTKPALSLS LFQSLKRDYA 

       730        740        750        760        770        780 
FVVDAAVEAG NVVKAVSSAD KKLIVGVQVF DIFTGASLGE GKKSIAVEVL LQPQDRTLTD 

       790        800 
EDLEALSKQI VASVAKQTGG VLRG

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References

[1]

"Whole-genome analyses of speciation events in pathogenic Brucellae."
Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E.
Infect. Immun. 73:8353-8361(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 2308.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM040264 Genomic DNA. Translation: CAJ12083.1.
RefSeq	YP_415448.1. NC_007618.1.
3D structure databases
ProteinModelPortal	Q2YQV4.
ModBase	Search...
Protein-protein interaction databases
STRING	359391.BAB1_2127.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAJ12083; CAJ12083; BAB1_2127.
GeneID	3788852.
KEGG	bmf:BAB1_2127.
PATRIC	17847353. VBIBruMel86222_2212.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0073.
HOGENOM	HOG000292085.
KO	K01890.
OMA	ELERKCK.
ProtClustDB	PRK00629.
Enzyme and pathway databases
BioCyc	BMEL359391:GJOQ-2181-MONOMER.
Family and domain databases
Gene3D	2.40.50.140. 1 hit. 3.30.56.20. 1 hit. 3.30.70.380. 1 hit. 3.50.40.10. 1 hit.
HAMAP	MF_00283. Phe_tRNA_synth_beta1.
InterPro	IPR005146. B3/B4_tRNA-bd. IPR009061. DNA-bd_dom_put. IPR012340. NA-bd_OB-fold. IPR004532. Phe-tRNA-ligase_IIc_bsu_bac. IPR020825. Phe-tRNA_synthase_B3/B4. IPR005121. PheS_beta_Fdx_antiC-bd. IPR002547. tRNA-bd_dom. IPR005147. tRNA_synthase_B5-dom. [Graphical view]
Pfam	PF03483. B3_4. 1 hit. PF03484. B5. 1 hit. PF03147. FDX-ACB. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
SMART	SM00873. B3_4. 1 hit. SM00874. B5. 1 hit. SM00896. FDX-ACB. 1 hit. [Graphical view]
SUPFAM	SSF56037. B3_4. 1 hit. SSF54991. Fdx_AntiC_bd. 1 hit. SSF50249. Nucleic_acid_OB. 1 hit. SSF46955. Putativ_DNA_bind. 1 hit.
TIGRFAMs	TIGR00472. pheT_bact. 1 hit.
PROSITE	PS51483. B5. 1 hit. PS51447. FDX_ACB. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFB_BRUA2

Accession

Primary (citable) accession number: Q2YQV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	February 7, 2006
Last modified:	May 1, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

Brucella abortus strain 2308

Brucella abortus (strain 2308): entries and gene names

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents