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Q2YQV1

- DNAJ_BRUA2

UniProt

Q2YQV1 - DNAJ_BRUA2

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Protein

Chaperone protein DnaJ

Gene
dnaJ, BAB1_2130
Organism
Brucella abortus (strain 2308)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins By similarity.UniRule annotation

Cofactori

Binds 2 zinc ions per monomer By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi148 – 1481Zinc 1 By similarity
Metal bindingi151 – 1511Zinc 1 By similarity
Metal bindingi165 – 1651Zinc 2 By similarity
Metal bindingi168 – 1681Zinc 2 By similarity
Metal bindingi187 – 1871Zinc 2 By similarity
Metal bindingi190 – 1901Zinc 2 By similarity
Metal bindingi201 – 2011Zinc 1 By similarity
Metal bindingi204 – 2041Zinc 1 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri135 – 21379CR-typeUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: InterPro
  2. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
  2. protein folding Source: InterPro
  3. response to heat Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

DNA replication, Stress response

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciBABO359391:GKDV-2184-MONOMER.
BMEL359391:GJOQ-2184-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein DnaJ
Gene namesi
Name:dnaJ
Ordered Locus Names:BAB1_2130
OrganismiBrucella abortus (strain 2308)
Taxonomic identifieri359391 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000002719: Chromosome I

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 377377Chaperone protein DnaJUniRule annotationPRO_1000085150Add
BLAST

Proteomic databases

PRIDEiQ2YQV1.

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi359391.BAB1_2130.

Structurei

3D structure databases

ProteinModelPortaliQ2YQV1.
SMRiQ2YQV1. Positions 2-77, 135-215.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 6966JAdd
BLAST
Repeati148 – 1558CXXCXGXG motifUniRule annotation
Repeati165 – 1728CXXCXGXG motifUniRule annotation
Repeati187 – 1948CXXCXGXG motifUniRule annotation
Repeati201 – 2088CXXCXGXG motifUniRule annotation

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi76 – 11843Gly-richUniRule annotationAdd
BLAST

Domaini

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the DnaJ family.
Contains 1 J domain.

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0484.
HOGENOMiHOG000226717.
KOiK03686.
OMAiGHTDPNQ.
OrthoDBiEOG6BPDKP.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPiMF_01152. DnaJ.
InterProiIPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
[Graphical view]
PfamiPF01556. CTDII. 1 hit.
PF00226. DnaJ. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
TIGRFAMsiTIGR02349. DnaJ_bact. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2YQV1-1 [UniParc]FASTAAdd to Basket

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MKIDYYEALG VTRTADDKTL KAAFRKLAMQ YHPDRNPDDP EAERKFKEIG    50
EAYETLKDPQ KRAAYDRFGH AAFENGGMGG GFGNGFGGAG GFADIFEDIF 100
GEMMGGGRRR SNGGRERGAD LRYNMEVTLE EAYAGKTAQI RVPTSITCDE 150
CSGSGAKPGS QPTTCTMCSG SGRVRAAQGF FSVERTCPGC NGRGQIIKDP 200
CEKCHGQGRV TQERSLSVNI PTGIEDGTRI RLAGEGEAGL RGGPAGDLYI 250
FLSVKPHEFF QRDGADLYCK VPISMTTAAL GGQFEVSTLD GTQTRVKVPE 300
GTQNGKQFRL KGKGMPVLRQ SVTGDLYIQI DIETPQNLSK RQRELLEEFE 350
KLSSQENSPK SAGFFSRMKE FFEGIGE 377
Length:377
Mass (Da):41,108
Last modified:December 20, 2005 - v1
Checksum:iE6771723F18CC630
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM040264 Genomic DNA. Translation: CAJ12086.1.
RefSeqiYP_415451.1. NC_007618.1.

Genome annotation databases

EnsemblBacteriaiCAJ12086; CAJ12086; BAB1_2130.
GeneIDi3788684.
KEGGibmf:BAB1_2130.
PATRICi17847361. VBIBruMel86222_2216.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM040264 Genomic DNA. Translation: CAJ12086.1 .
RefSeqi YP_415451.1. NC_007618.1.

3D structure databases

ProteinModelPortali Q2YQV1.
SMRi Q2YQV1. Positions 2-77, 135-215.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 359391.BAB1_2130.

Proteomic databases

PRIDEi Q2YQV1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAJ12086 ; CAJ12086 ; BAB1_2130 .
GeneIDi 3788684.
KEGGi bmf:BAB1_2130.
PATRICi 17847361. VBIBruMel86222_2216.

Phylogenomic databases

eggNOGi COG0484.
HOGENOMi HOG000226717.
KOi K03686.
OMAi GHTDPNQ.
OrthoDBi EOG6BPDKP.

Enzyme and pathway databases

BioCyci BABO359391:GKDV-2184-MONOMER.
BMEL359391:GJOQ-2184-MONOMER.

Miscellaneous databases

PROi Q2YQV1.

Family and domain databases

Gene3Di 1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPi MF_01152. DnaJ.
InterProi IPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
[Graphical view ]
Pfami PF01556. CTDII. 1 hit.
PF00226. DnaJ. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view ]
PRINTSi PR00625. JDOMAIN.
SMARTi SM00271. DnaJ. 1 hit.
[Graphical view ]
SUPFAMi SSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
TIGRFAMsi TIGR02349. DnaJ_bact. 1 hit.
PROSITEi PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 2308.

Entry informationi

Entry nameiDNAJ_BRUA2
AccessioniPrimary (citable) accession number: Q2YQV1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: December 20, 2005
Last modified: May 14, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella abortus strain 2308
    Brucella abortus (strain 2308): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi