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Q2YQR5 (TRUB_BRUA2) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA pseudouridine synthase B

EC=5.4.99.25
Alternative name(s):
tRNA pseudouridine(55) synthase
Short name=Psi55 synthase
tRNA pseudouridylate synthase
tRNA-uridine isomerase
Gene names
Name:truB
Ordered Locus Names:BAB1_2167
OrganismBrucella abortus (strain 2308) [Complete proteome] [HAMAP]
Taxonomic identifier359391 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length324 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs By similarity. HAMAP-Rule MF_01080

Catalytic activity

tRNA uridine55 = tRNA pseudouridine55. HAMAP-Rule MF_01080

Sequence similarities

Belongs to the pseudouridine synthase TruB family. Type 1 subfamily.

Ontologies

Keywords
   Biological processtRNA processing
   Molecular functionIsomerase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtRNA pseudouridine synthesis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: InterPro

pseudouridine synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 324324tRNA pseudouridine synthase B HAMAP-Rule MF_01080
PRO_0000229344

Sites

Active site491Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2YQR5 [UniParc].

Last modified February 7, 2006. Version 1.
Checksum: 850AF77186777988

FASTA32435,595
        10         20         30         40         50         60 
MARRGKKKGR PISGWVIFDK PKGMGSTEAV SKIKWLFSAE KAGHAGTLDP LASGMLPIAL 

        70         80         90        100        110        120 
GEATKTVPYV MDGTKVYRFT VTWGEERSTD DLEGQPTKTS DKRPSREEVE ALLPDYTGVI 

       130        140        150        160        170        180 
SQVPPQFSAI KIDGERAYDL AREGETVEIP AREVEIDRLE IVGFPDADRT EFEVECSKGT 

       190        200        210        220        230        240 
YVRSLARDMG RDLGCYGHIS DLRRVEVAPF TDEDMVTLAK LEAVWPPLPP KDEDGNVIEP 

       250        260        270        280        290        300 
APRRDFSALD ALVIDTGAAL DCLPQVPLSD DQAQRVRLGN PVILRGRDAP LEADEACVTT 

       310        320 
RGKLLAIGYI EHGQFKPKRV FTAG 

« Hide

References

[1]"Whole-genome analyses of speciation events in pathogenic Brucellae."
Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E.
Infect. Immun. 73:8353-8361(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 2308.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM040264 Genomic DNA. Translation: CAJ12123.1.
RefSeqYP_415487.1. NC_007618.1.

3D structure databases

ProteinModelPortalQ2YQR5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING359391.BAB1_2167.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAJ12123; CAJ12123; BAB1_2167.
GeneID3788582.
KEGGbmf:BAB1_2167.
PATRIC17847441. VBIBruMel86222_2256.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0130.
HOGENOMHOG000231225.
KOK03177.
OMAYELQFIR.
OrthoDBEOG6358D3.

Enzyme and pathway databases

BioCycBABO359391:GKDV-2221-MONOMER.
BMEL359391:GJOQ-2221-MONOMER.

Family and domain databases

HAMAPMF_01080. TruB_bact.
InterProIPR002501. PsdUridine_synth.
IPR020103. PsdUridine_synth_cat_dom.
IPR014780. tRNA_psdUridine_synth_TruB.
IPR015240. tRNA_sdUridine_synth_fam1_C.
[Graphical view]
PANTHERPTHR13767. PTHR13767. 1 hit.
PfamPF09157. TruB-C_2. 1 hit.
PF01509. TruB_N. 1 hit.
[Graphical view]
SUPFAMSSF55120. SSF55120. 1 hit.
TIGRFAMsTIGR00431. TruB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRUB_BRUA2
AccessionPrimary (citable) accession number: Q2YQR5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: February 7, 2006
Last modified: May 14, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Brucella abortus strain 2308

Brucella abortus (strain 2308): entries and gene names