Reviewed, UniProtKB/Swiss-Prot Q2YQN2 (ILVC_BRUA2)

Names and origin

Protein names

Recommended name:
    Ketol-acid reductoisomerase
    EC=1.1.1.86
Alternative name(s):
    Acetohydroxy-acid isomeroreductase
    Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	BAB1_1399

Organism

Brucella abortus (strain 2308) [Complete proteome] [HAMAP]

Taxonomic identifier

359391 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_0000226164
Sites
	Active site	108	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q2YQN2-1 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: E1A8D9C2C1E4E423	FASTA	339	37,048
	10 20 30 40 50 60 MRVYYDRDAD VNLIKSKKVV IVGYGSQGRA HALNLKDSGA ANVRVALREG SATVQKAQAD 70 80 90 100 110 120 GFEVMNVADA AKWADLMMMA TPDELQADIY RDHIHNNLRD GAAIAFAHGL NVHCGLIEPK 130 140 150 160 170 180 KTVDVVMIAP KGPGHTVRGE YQKGGGVPCL IAIHQDASGN AHDLALSYAS GVGGGRSGVI 190 200 210 220 230 240 ETTFKEECET DLFGEQAVLC GGVVELIRTG FEVLVEAGYA PEMAYFECLN EMKLIVDLIY 250 260 270 280 290 300 EGGIANMNYS ISNTAEWGEY VTGPRIITAE TKAEMKRVLK DIQTGKFTSD WMQEWKAGAA 310 320 330 RFKGIRRLND AHQIEEVGGK LRAMMPWIEK NKLVDKARN

References

[1]

"Whole-genome analyses of speciation events in pathogenic Brucellae." Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E. Infect. Immun. 73:8353-8361(2005) [PubMed: 16299333] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM040264 Genomic DNA. Translation: CAJ11355.1.
RefSeq	YP_414771.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3787965.
GenomeReviews	Gene locus BAB1_1399 in contig AM040264_GR.
KEGG	bmf:BAB1_1399.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2YQN2.
OMA	Q2YQN2. AHGFNIR.
Enzyme and pathway databases
BioCyc	BMEL359391:BAB1_1399-MON.
Family and domain databases
HAMAP	MF_00435. [Tree]
InterPro	IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013116. IlvN. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
TIGRFAMs	TIGR00465. ilvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_BRUA2

Accession

Primary (citable) accession number: Q2YQN2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	February 7, 2006
Last modified:	June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)