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Protein

Pyridoxine 5'-phosphate synthase

Gene

pdxJ

Organism
Brucella abortus (strain 2308)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate.UniRule annotation

Catalytic activityi

1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate = pyridoxine 5'-phosphate + phosphate + 2 H2O.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei8 – 813-amino-2-oxopropyl phosphateUniRule annotation
Binding sitei19 – 1913-amino-2-oxopropyl phosphateUniRule annotation
Active sitei44 – 441Proton acceptorUniRule annotation
Binding sitei46 – 4611-deoxy-D-xylulose 5-phosphateUniRule annotation
Binding sitei51 – 5111-deoxy-D-xylulose 5-phosphateUniRule annotation
Active sitei76 – 761Proton acceptorUniRule annotation
Binding sitei106 – 10611-deoxy-D-xylulose 5-phosphateUniRule annotation
Sitei157 – 1571Transition state stabilizerUniRule annotation
Active sitei198 – 1981Proton donorUniRule annotation
Binding sitei199 – 19913-amino-2-oxopropyl phosphate; via amide nitrogenUniRule annotation

GO - Molecular functioni

  1. pyridoxine 5'-phosphate synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. pyridoxine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Pyridoxine biosynthesis

Enzyme and pathway databases

BioCyciBABO359391:GKDV-1434-MONOMER.
BMEL359391:GJOQ-1434-MONOMER.
UniPathwayiUPA00244; UER00313.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyridoxine 5'-phosphate synthaseUniRule annotation (EC:2.6.99.2UniRule annotation)
Short name:
PNP synthaseUniRule annotation
Gene namesi
Name:pdxJUniRule annotation
Ordered Locus Names:BAB1_1404
OrganismiBrucella abortus (strain 2308)
Taxonomic identifieri359391 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000002719 Componenti: Chromosome I

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 246246Pyridoxine 5'-phosphate synthasePRO_0000231791Add
BLAST

Interactioni

Subunit structurei

Homooctamer; tetramer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi359391.BAB1_1404.

Structurei

3D structure databases

ProteinModelPortaliQ2YQM7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni221 – 22223-amino-2-oxopropyl phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the PNP synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0854.
HOGENOMiHOG000258095.
KOiK03474.
OMAiGMEELNI.
OrthoDBiEOG6M9F0H.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00279. PdxJ.
InterProiIPR013785. Aldolase_TIM.
IPR004569. PyrdxlP_synth_PdxJ.
[Graphical view]
PfamiPF03740. PdxJ. 1 hit.
[Graphical view]
SUPFAMiSSF63892. SSF63892. 1 hit.
TIGRFAMsiTIGR00559. pdxJ. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2YQM7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAKLSVNLN AIAMLRNRRD LPWPSVTGLG RAALAAGAAG LTVHPRPDQR
60 70 80 90 100
HIRFSDLGDI RALIDDEYPQ AEFNIEGFPS EAFLDLVEKH EPEQVTLVPD
110 120 130 140 150
DPMQATSDHG WDFMSKADFL APIVARLKGR GMRVSLFADP DSLGYERAKA
160 170 180 190 200
IGADRVELYT GPYGATHDDP AAAARELDRL EKAARAATAL GLAVNAGHDL
210 220 230 240
TVDNLPALVK RIPQLAEVSI GHGLTADALM YGIPVTVSRY ITALAG
Length:246
Mass (Da):26,466
Last modified:February 7, 2006 - v1
Checksum:i7AEF11306AE7B475
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM040264 Genomic DNA. Translation: CAJ11360.1.
RefSeqiYP_414776.1. NC_007618.1.

Genome annotation databases

EnsemblBacteriaiCAJ11360; CAJ11360; BAB1_1404.
KEGGibmf:BAB1_1404.
PATRICi17845792. VBIBruMel86222_1459.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM040264 Genomic DNA. Translation: CAJ11360.1.
RefSeqiYP_414776.1. NC_007618.1.

3D structure databases

ProteinModelPortaliQ2YQM7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi359391.BAB1_1404.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ11360; CAJ11360; BAB1_1404.
KEGGibmf:BAB1_1404.
PATRICi17845792. VBIBruMel86222_1459.

Phylogenomic databases

eggNOGiCOG0854.
HOGENOMiHOG000258095.
KOiK03474.
OMAiGMEELNI.
OrthoDBiEOG6M9F0H.

Enzyme and pathway databases

UniPathwayiUPA00244; UER00313.
BioCyciBABO359391:GKDV-1434-MONOMER.
BMEL359391:GJOQ-1434-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00279. PdxJ.
InterProiIPR013785. Aldolase_TIM.
IPR004569. PyrdxlP_synth_PdxJ.
[Graphical view]
PfamiPF03740. PdxJ. 1 hit.
[Graphical view]
SUPFAMiSSF63892. SSF63892. 1 hit.
TIGRFAMsiTIGR00559. pdxJ. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 2308.

Entry informationi

Entry nameiPDXJ_BRUA2
AccessioniPrimary (citable) accession number: Q2YQM7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: February 7, 2006
Last modified: April 1, 2015
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Brucella abortus strain 2308
    Brucella abortus (strain 2308): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.