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Protein

Urease subunit gamma 2

Gene

ureA2

Organism
Brucella abortus (strain 2308)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Disrupting the ure2 operon has no effect on urease activity or pathogen survival in BALB/c mice when administered orally.

Catalytic activityi

Urea + H2O = CO2 + 2 NH3.UniRule annotation

Pathwayi: urea degradation

This protein is involved in step 1 of the subpathway that synthesizes CO(2) and NH(3) from urea (urease route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Urease subunit gamma 2 (ureA2), Urease subunit beta 1 (ureB1), Urease subunit gamma 1 (ureA1), Urease subunit alpha 1 (ureC1), Urease subunit beta 2 (ureB2), Urease subunit alpha 2 (ureC2)
This subpathway is part of the pathway urea degradation, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (urease route), the pathway urea degradation and in Nitrogen metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciBABO359391:GKDV-1406-MONOMER.
BMEL359391:GJOQ-1406-MONOMER.
UniPathwayiUPA00258; UER00370.

Names & Taxonomyi

Protein namesi
Recommended name:
Urease subunit gamma 2UniRule annotation (EC:3.5.1.5UniRule annotation)
Alternative name(s):
Urea amidohydrolase subunit gamma 2UniRule annotation
Gene namesi
Name:ureA2UniRule annotation
Ordered Locus Names:BAB1_1376
OrganismiBrucella abortus (strain 2308)
Taxonomic identifieri359391 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000002719 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 100100Urease subunit gamma 2PRO_0000234200Add
BLAST

Proteomic databases

PRIDEiQ2YQE0.

Interactioni

Subunit structurei

Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.UniRule annotation

Structurei

Secondary structure

1
100
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 2521Combined sources
Helixi32 – 4817Combined sources
Helixi53 – 608Combined sources
Turni61 – 633Combined sources
Helixi66 – 683Combined sources
Helixi73 – 764Combined sources
Beta strandi78 – 869Combined sources
Beta strandi89 – 979Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4FURX-ray2.10A/B/C/D/E/F1-100[»]
ProteinModelPortaliQ2YQE0.
SMRiQ2YQE0. Positions 1-100.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the urease gamma subunit family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000075053.
KOiK01430.
OMAiMADASHA.
OrthoDBiEOG69PQ9G.

Family and domain databases

Gene3Di3.30.280.10. 1 hit.
HAMAPiMF_00739. Urease_gamma.
InterProiIPR012010. Urease_gamma.
IPR002026. Urease_gamma/gamma-beta_su.
[Graphical view]
PfamiPF00547. Urease_gamma. 1 hit.
[Graphical view]
PIRSFiPIRSF001223. Urease_gamma. 1 hit.
ProDomiPD002319. Urease_gamma_reg. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF54111. SSF54111. 1 hit.
TIGRFAMsiTIGR00193. urease_gam. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2YQE0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHLTPREFDK LVIHMLSDVA LKRKNKGLKL NHPEAVAVLS AYVLDGAREG
60 70 80 90 100
KTVEEVMDGA RSVLKADDVM DGVPDLLPLI QVEAVFSDGS RLVSLHNPIT
Length:100
Mass (Da):10,943
Last modified:December 20, 2005 - v1
Checksum:iFABF969BACC53D05
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM040264 Genomic DNA. Translation: CAJ11332.1.
RefSeqiWP_002964469.1. NZ_KN046823.1.

Genome annotation databases

EnsemblBacteriaiCAJ11332; CAJ11332; BAB1_1376.
GeneIDi3789046.
KEGGibmf:BAB1_1376.
PATRICi17845734. VBIBruMel86222_1430.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM040264 Genomic DNA. Translation: CAJ11332.1.
RefSeqiWP_002964469.1. NZ_KN046823.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4FURX-ray2.10A/B/C/D/E/F1-100[»]
ProteinModelPortaliQ2YQE0.
SMRiQ2YQE0. Positions 1-100.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ2YQE0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ11332; CAJ11332; BAB1_1376.
GeneIDi3789046.
KEGGibmf:BAB1_1376.
PATRICi17845734. VBIBruMel86222_1430.

Phylogenomic databases

HOGENOMiHOG000075053.
KOiK01430.
OMAiMADASHA.
OrthoDBiEOG69PQ9G.

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.
BioCyciBABO359391:GKDV-1406-MONOMER.
BMEL359391:GJOQ-1406-MONOMER.

Family and domain databases

Gene3Di3.30.280.10. 1 hit.
HAMAPiMF_00739. Urease_gamma.
InterProiIPR012010. Urease_gamma.
IPR002026. Urease_gamma/gamma-beta_su.
[Graphical view]
PfamiPF00547. Urease_gamma. 1 hit.
[Graphical view]
PIRSFiPIRSF001223. Urease_gamma. 1 hit.
ProDomiPD002319. Urease_gamma_reg. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF54111. SSF54111. 1 hit.
TIGRFAMsiTIGR00193. urease_gam. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 2308.
  2. "Characterization of the urease operon of Brucella abortus and assessment of its role in virulence of the bacterium."
    Sangari F.J., Seoane A., Rodriguez M.C., Aguero J., Garcia Lobo J.M.
    Infect. Immun. 75:774-780(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: LACK OF ROLE IN VIRULENCE.

Entry informationi

Entry nameiURE32_BRUA2
AccessioniPrimary (citable) accession number: Q2YQE0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: December 20, 2005
Last modified: November 11, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Brucella abortus strain 2308
    Brucella abortus (strain 2308): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.