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Protein

Cell cycle response regulator CtrA

Gene

ctrA

Organism
Brucella abortus (strain 2308)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of a regulatory phosphorelay system that controls B.abortus cell growth, division, and intracellular survival inside mammalian host cells. This signaling pathway is composed of CckA, ChpT, CtrA and CpdR. CtrA is a response regulator substrate of ChpT (PubMed:26124143). When phosphorylated, directly regulates the expression of ccrM (PubMed:10852881). Is also probably involved in the transcriptional regulation of rpoD, pleC, minC and ftsE genes (By similarity).By similarity1 Publication1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi124 – 223OmpR/PhoB-typePROSITE-ProRule annotationAdd BLAST100

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cell cycle response regulator CtrA
Alternative name(s):
Cell cycle transcriptional regulator A
Gene namesi
Name:ctrA1 Publication
Ordered Locus Names:BAB1_1614
OrganismiBrucella abortus (strain 2308)
Taxonomic identifieri359391 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000002719 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

This gene cannot be deleted or disrupted, indicating this gene is essential in B.abortus.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi15S → R: 90% reduction in phosphoryl transfer from CckA-P to CtrA via ChpT. 1 Publication1
Mutagenesisi148V → F: Temperature sensitive mutant that displays no growth defect at 30 degrees Celsius but fails to replicate when grown at 37 degrees Celsius. These mutant cells cultured at the nonpermissive temperature are abnormally elongated and show defects in cell divison by 24 hours, with apparent budding from the midcell position. Significant reduction in intracellular B.abortus replication and/or survival in human macrophages. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003631961 – 232Cell cycle response regulator CtrAAdd BLAST232

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei514-aspartylphosphatePROSITE-ProRule annotation1 Publication1

Post-translational modificationi

Is phosphorylated by ChpT-P, likely on Asp-51.1 Publication

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Forms an asymmetric heterotetramer with ChpT (2:2). There are at least two modes of interaction between ChpT and CtrA, only one of which is competent to catalyze His-Asp phosphoryl transfer.1 Publication

Structurei

Secondary structure

1232
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 6Combined sources5
Helixi10 – 21Combined sources12
Turni22 – 24Combined sources3
Beta strandi26 – 31Combined sources6
Helixi33 – 42Combined sources10
Beta strandi46 – 52Combined sources7
Beta strandi55 – 57Combined sources3
Helixi59 – 68Combined sources10
Beta strandi75 – 81Combined sources7
Helixi84 – 93Combined sources10
Beta strandi96 – 102Combined sources7
Helixi105 – 116Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4QPJX-ray2.74C/D1-118[»]
ProteinModelPortaliQ2YQA4.
SMRiQ2YQA4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 116Response regulatoryPROSITE-ProRule annotationAdd BLAST115

Sequence similaritiesi

Contains 1 OmpR/PhoB-type DNA-binding domain.PROSITE-ProRule annotation
Contains 1 response regulatory domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000034815.
KOiK13584.
OMAiYRTEVAY.

Family and domain databases

CDDicd00383. trans_reg_C. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR001867. OmpR/PhoB-type_DNA-bd.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]
SMARTiSM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]
SUPFAMiSSF52172. SSF52172. 1 hit.
PROSITEiPS51755. OMPR_PHOB. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2YQA4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVLLIEDDS AIAQSIELML KSESFNVYTT DLGEEGIDLG KLYDYDIILL
60 70 80 90 100
DLNLPDMSGY EVLRTLRLSK VKTPILILSG MAGIEDKVRG LGFGADDYMT
110 120 130 140 150
KPFHKDELIA RIHAIVRRSK GHAQSVITTG DLVVNLDAKT VEVAGQRVHL
160 170 180 190 200
TGKEYQMLEL LSLRKGTTLT KEMFLNHLYG GMDEPELKII DVFICKLRKK
210 220 230
LDAVSGNQSY IETVWGRGYV LREPDAEMRE SA
Length:232
Mass (Da):26,066
Last modified:February 7, 2006 - v1
Checksum:i9177C2281EC76ACE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM040264 Genomic DNA. Translation: CAJ11570.1.
RefSeqiWP_002964699.1. NZ_KN046823.1.

Genome annotation databases

EnsemblBacteriaiCAJ11570; CAJ11570; BAB1_1614.
GeneIDi3788674.
KEGGibmf:BAB1_1614.
PATRICi17846224. VBIBruMel86222_1673.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM040264 Genomic DNA. Translation: CAJ11570.1.
RefSeqiWP_002964699.1. NZ_KN046823.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4QPJX-ray2.74C/D1-118[»]
ProteinModelPortaliQ2YQA4.
SMRiQ2YQA4.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ11570; CAJ11570; BAB1_1614.
GeneIDi3788674.
KEGGibmf:BAB1_1614.
PATRICi17846224. VBIBruMel86222_1673.

Phylogenomic databases

HOGENOMiHOG000034815.
KOiK13584.
OMAiYRTEVAY.

Family and domain databases

CDDicd00383. trans_reg_C. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR001867. OmpR/PhoB-type_DNA-bd.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]
SMARTiSM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]
SUPFAMiSSF52172. SSF52172. 1 hit.
PROSITEiPS51755. OMPR_PHOB. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCTRA_BRUA2
AccessioniPrimary (citable) accession number: Q2YQA4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: February 7, 2006
Last modified: November 2, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Brucella abortus strain 2308
    Brucella abortus (strain 2308): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.