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Protein

Beta-(1-->2)glucan export ATP-binding/permease protein NdvA

Gene

ndvA

Organism
Brucella abortus (strain 2308)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in beta-(1-->2)glucan export. Its export to the periplasmic space is required to exert its action as a virulence factor. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation (Probable).1 Publication

Catalytic activityi

ATP + H2O + beta-glucan(In) = ADP + phosphate + beta-glucan(Out).UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi368 – 3758ATPUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Sugar transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBABO359391:GKDV-1036-MONOMER.
BMEL359391:GJOQ-1036-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-(1-->2)glucan export ATP-binding/permease protein NdvAUniRule annotation (EC:3.6.3.42UniRule annotation)
Gene namesi
Name:ndvAUniRule annotation
Synonyms:cgt
Ordered Locus Names:BAB1_1017
OrganismiBrucella abortus (strain 2308)
Taxonomic identifieri359391 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000002719 Componenti: Chromosome I

Subcellular locationi

  • Cell inner membrane UniRule annotation1 Publication; Multi-pass membrane protein UniRule annotation1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei22 – 4221HelicalUniRule annotationAdd
BLAST
Transmembranei55 – 7521HelicalUniRule annotationAdd
BLAST
Transmembranei156 – 17621HelicalUniRule annotationAdd
BLAST
Transmembranei248 – 26821HelicalUniRule annotationAdd
BLAST
Transmembranei276 – 29621HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 599599Beta-(1-->2)glucan export ATP-binding/permease protein NdvAPRO_0000290245Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ2YQ73.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 301281ABC transmembrane type-1UniRule annotationAdd
BLAST
Domaini335 – 569235ABC transporterUniRule annotationAdd
BLAST

Domaini

In NdvA the ATP-binding domain (NBD) and the transmembrane domain (TMD) are fused.

Sequence similaritiesi

Belongs to the ABC transporter superfamily. Beta-(1-->2)glucan exporter (TC 3.A.1.108.1) family. [View classification]UniRule annotation
Contains 1 ABC transmembrane type-1 domain.UniRule annotation
Contains 1 ABC transporter domain.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1132.
KOiK06147.
OMAiCVVFTVL.
OrthoDBiEOG6T7N3V.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR017902. ABC_bGlucan_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR005896. NdvA.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
TIGRFAMsiTIGR01192. chvA. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51317. NDVA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2YQ73-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLLKIYWRA MQYLAVERTA TITMCVASVL VALVTLAEPV LFGRVIQSIS
60 70 80 90 100
DKGDIFSPLL MWAALGGFNI MAAVFVARGA DRLAHRRRLG VMIDSYERLI
110 120 130 140 150
TMPLAWHQKR GTSNALHTLI RATDSLFTLW LEFMRQHLTT VVALATLIPV
160 170 180 190 200
AMTMDMRMSL VLIVLGVIYV MIGQLVMRKT KDGQAAVEKH HHKLFEHVSD
210 220 230 240 250
TISNVSVVQS YNRIASETQA LRDYAKNLEN AQFPVLNWWA LASGLNRMAS
260 270 280 290 300
TFSMVVVLVL GAYFVTKGQM RVGDVIAFIG FAQLMIGRLD QISAFINQTV
310 320 330 340 350
TARAKLEEFF QMEDATADRQ EPENVADLND VKGDIVFDNV TYEFPNSGQG
360 370 380 390 400
VYDVSFEVKP GQTVAIVGPT GAGKTTLINL LQRVFDPAAG RIMIDGTDTR
410 420 430 440 450
TVSRRSLRHA IATVFQDAGL FNRSVEDNIR VGRANATHEE VHAAAKAAAA
460 470 480 490 500
HDFILAKSEG YDTFVGERGS QLSGGERQRL AIARAILKDS PILVLDEATS
510 520 530 540 550
ALDVETEEKV TQAVDELSHN RTTFIIAHRL STVRSADLVL FMDKGHLVES
560 570 580 590
GSFNELAERG GRFSDLLRAG GLKLEDKQPK QPVVEGSNVM PFPVKGAVA
Length:599
Mass (Da):65,951
Last modified:February 7, 2006 - v1
Checksum:i2626C3220E57341C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY237159 Genomic DNA. Translation: AAO84919.1.
AM040264 Genomic DNA. Translation: CAJ10973.1.

Genome annotation databases

EnsemblBacteriaiCAJ10973; CAJ10973; BAB1_1017.
KEGGibmf:BAB1_1017.
PATRICi17844980. VBIBruMel86222_1062.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY237159 Genomic DNA. Translation: AAO84919.1.
AM040264 Genomic DNA. Translation: CAJ10973.1.

3D structure databases

ProteinModelPortaliQ2YQ73.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ10973; CAJ10973; BAB1_1017.
KEGGibmf:BAB1_1017.
PATRICi17844980. VBIBruMel86222_1062.

Phylogenomic databases

eggNOGiCOG1132.
KOiK06147.
OMAiCVVFTVL.
OrthoDBiEOG6T7N3V.

Enzyme and pathway databases

BioCyciBABO359391:GKDV-1036-MONOMER.
BMEL359391:GJOQ-1036-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR017902. ABC_bGlucan_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR005896. NdvA.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
TIGRFAMsiTIGR01192. chvA. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51317. NDVA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of cgt, the Brucella abortus cyclic beta-1,2-glucan transporter gene, and its role in virulence."
    Roset M.S., Ciocchini A.E., Ugalde R.A., Inon de Iannino N.
    Infect. Immun. 72:2263-2271(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN BETA-(1->2)GLUCAN TRANSPORT, SUBCELLULAR LOCATION.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 2308.

Entry informationi

Entry nameiNDVA_BRUA2
AccessioniPrimary (citable) accession number: Q2YQ73
Secondary accession number(s): Q57DD0, Q844Y8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 12, 2007
Last sequence update: February 7, 2006
Last modified: July 22, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Brucella abortus strain 2308
    Brucella abortus (strain 2308): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.