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Reviewed, UniProtKB/Swiss-Prot Q2YPX3 (LON_BRUA2)

Last modified June 16, 2009. Version 27. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    ATP-dependent protease La
    EC=3.4.21.53
Gene names
Name: lon
Ordered Locus Names: BAB1_1130
OrganismBrucella abortus (strain 2308) [Complete proteome] [HAMAP]
Taxonomic identifier359391 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length812 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

Degrades short-lived regulatory and abnormal proteins in presence of ATP. Hydrolyzes two ATPs for each peptide bond cleaved in the protein substrate. Required for wild-type virulence during the initial stages of infection in the mouse model, but not essential for the establishment and maintenance of chronic infection in this host. Ref.1

Catalytic activity

Hydrolysis of proteins in presence of ATP.

Subunit structure

Homotetramer By similarity.

Subcellular location

Cytoplasm By similarity.

Induction

By stress conditions (acid shock, heat shock, ethanol and puromycin). Ref.1

Sequence similarities

Belongs to the peptidase S16 family.

Contains 1 Lon domain.

Ontologies

Keywords
   Biological processStress response
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionHydrolase
Protease
Serine protease
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processproteolysis

Inferred from electronic annotation. Source: InterPro

response to stress

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ATP-dependent peptidase activity

Inferred from electronic annotation. Source: InterPro

serine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 812812ATP-dependent protease La
PRO_0000076122

Regions

Domain23 – 213191Lon
Nucleotide binding367 – 3748ATP Potential

Sites

Active site6891 By similarity
Active site7321 By similarity

Experimental info

Sequence conflict2271R → P in AAB97420. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q2YPX3-1 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: DB30B60818FF6AAE

FASTA81289,919
        10         20         30         40         50         60 
MTGIEQKTPV GGSETGGADG LYAVLPLRDI VVFPHMIVPL FVGREKSIRA LEEVMGVDKQ 

        70         80         90        100        110        120 
ILLATQKNAA DDDPAPDAIY EIGTIANVLQ LLKLPDGTVK VLVEGTARAK ISKFTDREDY 

       130        140        150        160        170        180 
HEAYAAALQE PEEDAVEIEA LARSVVPDFE NYVKLNKKIS PEVVGAASQI DDYSKLADTV 

       190        200        210        220        230        240 
ASHLAIKIPE KQEMLSVLSV RERLEKALSF MEAEISVLQV EKRIRSRVKR QMEKTQREYY 

       250        260        270        280        290        300 
LNEQMKAIQK ELGDSEDGRD EVAEIEERIT KTKLSKEARE KALAELKKLR SMSPMSAEAT 

       310        320        330        340        350        360 
VVRNYLDWLL SIPWGKKSKV KQDLNFAQEV LDAEHFGLGK VKERIVEYLA VQARSTKIKG 

       370        380        390        400        410        420 
PILCLVGPPG VGKTSLARSI AKATGREYVR MSLGGVRDEA EIRGHRRTYI GSMPGKVIQS 

       430        440        450        460        470        480 
MKKAKKSNPL FLLDEIDKMG QDFRGDPSSA MLEVLDPEQN ATFMDHYLEV EYDLSNVMFV 

       490        500        510        520        530        540 
TTANTMNIPV PLLDRMEIIR IAGYTEDEKL EIAKRHLLPK AIKDHALQPK EFSVTEDALR 

       550        560        570        580        590        600 
NVIRHYTREA GVRSLEREVM TLARKAVTEI LKTKKKSVKI TDKNLSDYLG VEKFRFGQID 

       610        620        630        640        650        660 
GEDQVGVVTG LAWTEVGGEL LTIEGVMMPG KGRMTVTGNL RDVMKESISA AASYVRSRAI 

       670        680        690        700        710        720 
DFGIEPPLFD KRDIHVHVPE GATPKDGPSA GIAMVTAIVS VLTGIPVRKD IAMTGEVTLR 

       730        740        750        760        770        780 
GRVLPIGGLK EKLLATLRGG IKKVLIPEEN AKDLAEIPDN VKNNLEIVPV SRVGEVLKHA 

       790        800        810 
LVRQPEPIEW TEQENPTAVP PVEDEAGASL AH 

« Hide

References

« Hide 'large scale' references
[1]"The Brucella abortus Lon functions as a generalized stress response protease and is required for wild-type virulence in BALB/c mice."
Robertson G.T., Kovach M.E., Allen C.A., Ficht T.A., Roop R.M. II
Mol. Microbiol. 35:577-588(2000) [PubMed: 10672180] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION.
[2]"Whole-genome analyses of speciation events in pathogenic Brucellae."
Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E.
Infect. Immun. 73:8353-8361(2005) [PubMed: 16299333] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

AF042348 Genomic DNA. Translation: AAB97420.1.
AM040264 Genomic DNA. Translation: CAJ11086.1.
RefSeqYP_414523.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID3787778.
GenomeReviewsGene locus BAB1_1130 in contig AM040264_GR.
KEGGbmf:BAB1_1130.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ2YPX3.
OMAQ2YPX3. VMKESIQ.

Enzyme and pathway databases

BioCycBMEL359391:BAB1_1130-MON.

Family and domain databases

InterProIPR003593. ATPase_AAA+_core.
IPR003959. ATPase_AAA_core.
IPR008269. Pept_S16_C.
IPR004815. Pept_S16_lon.
IPR003111. Pept_S16_N.
IPR008268. Peptidase_S16_AS.
IPR001984. Peptidase_S16_C.
[Graphical view]
PfamPF00004. AAA. 1 hit.
PF02190. LON. 1 hit.
PF05362. Lon_C. 1 hit.
[Graphical view]
PRINTSPR00830. ENDOLAPTASE.
SMARTSM00382. AAA. 1 hit.
SM00464. LON. 1 hit.
[Graphical view]
TIGRFAMsTIGR00763. lon. 1 hit.
PROSITEPS01046. LON_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLON_BRUA2
AccessionPrimary (citable) accession number: Q2YPX3
Secondary accession number(s): O52605, Q57D31
Entry history
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: December 20, 2005
Last modified: June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Brucella abortus strain 2308

Brucella abortus (strain 2308): entries and gene names

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents