Q2YPU9 (KDSA_BRUA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-dehydro-3-deoxyphosphooctonate aldolase
EC=2.5.1.55

Alternative name(s):
3-deoxy-D-manno-octulosonic acid 8-phosphate synthase
KDO-8-phosphate synthase
Short name=KDO 8-P synthase
Short name=KDOPS
Phospho-2-dehydro-3-deoxyoctonate aldolase

Gene names

Name:	kdsA
Ordered Locus Names:	BAB1_1156

Organism

Brucella abortus (strain 2308) [Complete proteome] [HAMAP]

Taxonomic identifier

359391 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella abortus

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + D-arabinose 5-phosphate + H2O = 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate. HAMAP-Rule MF_00056
Pathway	Carbohydrate biosynthesis; 3-deoxy-D-manno-octulosonate biosynthesis; 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate: step 2/3. HAMAP-Rule MF_00056 Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP-Rule MF_00056
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the KdsA family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	keto-3-deoxy-D-manno-octulosonic acid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	3-deoxy-8-phosphooctulonate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 277	277	2-dehydro-3-deoxyphosphooctonate aldolase HAMAP-Rule MF_00056		PRO_0000304436

Sequences

	Sequence		Length	Mass (Da)
	Q2YPU9 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 1C754D90382F0DD8	FASTA	277	29,532
	10 20 30 40 50 60 MVTANSTVKV GNVTFSNSAP LALIAGPCQM ETRDHAFEMA GRLKEMTDKL GIGLVYKSSF 70 80 90 100 110 120 DKANRTSLKA ARGIGLEKAL EVFSDLKKEY GFPVLTDIHT EEQCAAVAPV VDVLQIPAFL 130 140 150 160 170 180 CRQTDLLIAA ARTGRVVNVK KGQFLAPWDM KNVLAKITES GNPNVLATER GVSFGYNTLV 190 200 210 220 230 240 SDMRALPIMA GLGAPVIFDA THSVQQPGGQ GGSTGGQREF VETLARAAVA VGVAGFFIET 250 260 270 HEDPDNAPSD GPNMVPIDKM PALLEKLMAF DRIAKAL

References

[1]

"Whole-genome analyses of speciation events in pathogenic Brucellae." Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E. Infect. Immun. 73:8353-8361(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 2308.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM040264 Genomic DNA. Translation: CAJ11112.1.
RefSeq	YP_414547.1. NC_007618.1.
3D structure databases
HSSP	HSSP built from PDB template 2A21 based on UniProtKB O66496.
ProteinModelPortal	Q2YPU9.
ModBase	Search...
Protein-protein interaction databases
STRING	359391.BAB1_1156.
Proteomic databases
PRIDE	Q2YPU9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAJ11112; CAJ11112; BAB1_1156.
GeneID	3787797.
KEGG	bmf:BAB1_1156.
PATRIC	17845269. VBIBruMel86222_1204.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2877.
HOGENOM	HOG000023021.
KO	K01627.
OMA	HGLFIET.
ProtClustDB	PRK05198.
Enzyme and pathway databases
BioCyc	BMEL359391:GJOQ-1178-MONOMER.
UniPathway	UPA00030. UPA00357; UER00474.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_00056. KDO8P_synth.
InterPro	IPR013785. Aldolase_TIM. IPR006218. DAHP1/KDSA. IPR006269. KDO8P_synthase. [Graphical view]
PANTHER	PTHR21057:SF2. PTHR21057:SF2. 1 hit.
Pfam	PF00793. DAHP_synth_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01362. KDO8P_synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSA_BRUA2

Accession

Primary (citable) accession number: Q2YPU9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	February 7, 2006
Last modified:	May 1, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program