Reviewed, UniProtKB/Swiss-Prot Q2YPQ5 (KDSB_BRUA2)

Names and origin

Protein names

Recommended name:
    3-deoxy-manno-octulosonate cytidylyltransferase
    EC=2.7.7.38
Alternative name(s):
    CMP-KDO synthetase
    CMP-2-keto-3-deoxyoctulosonic acid synthetase
      Short name=CKS

Gene names

Name:	kdsB
Ordered Locus Names:	BAB1_0035

Organism

Brucella abortus (strain 2308) [Complete proteome] [HAMAP]

Taxonomic identifier

359391 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria By similarity.
Catalytic activity	CTP + 3-deoxy-D-manno-octulosonate = diphosphate + CMP-3-deoxy-D-manno-octulosonate. HAMAP MF_00057
Pathway	Nucleotide-sugar biosynthesis; CMP-3-deoxy-D-manno-octulosonate biosynthesis; CMP-3-deoxy-D-manno-octulosonate from 3-deoxy-D-manno-octulosonate and CTP: step 1/1. HAMAP MF_00057 Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP MF_00057
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the kdsB family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cytoplasm
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	keto-3-deoxy-D-manno-octulosonic acid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-manno-octulosonate cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 251	251	3-deoxy-manno-octulosonate cytidylyltransferase HAMAP MF_00057		PRO_0000370012

Sequences

	Sequence		Length	Mass (Da)
	Q2YPQ5-1 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 945714A32037C6A7	FASTA	251	27,304
	10 20 30 40 50 60 MLQTMKTLTL IPARLGSTRL PNKPLADICG KPMIVHVADR AAAAKLGRTV IATDSEEIFK 70 80 90 100 110 120 VVAAHGHEAI MTRGDHESGS DRIYEALAKL DPSGEIDAVV NVQGDLPTID PDTIRRALLP 130 140 150 160 170 180 LEDGPADIAT LGVEITVEEE KTNPNVVKIV GSPLAGNRRL RALYFTRATA PYGEGPLYHH 190 200 210 220 230 240 IGLYAYRRSA LERFVKLGPS PLEKREKLEQ LRALEAGMRI DVEIVKTVPL GVDTQADLDR 250 ARTFCSQAGT I

References

[1]

"Whole-genome analyses of speciation events in pathogenic Brucellae." Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E. Infect. Immun. 73:8353-8361(2005) [PubMed: 16299333] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM040264 Genomic DNA. Translation: CAJ09991.1.
RefSeq	YP_413538.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2YPQ5.
Genome annotation databases
GeneID	3786850.
GenomeReviews	Gene locus BAB1_0035 in contig AM040264_GR.
KEGG	bmf:BAB1_0035.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2YPQ5.
OMA	TDRINEA.
Enzyme and pathway databases
BioCyc	BMEL359391:BAB1_0035-MON.
Family and domain databases
HAMAP	MF_00057. [Tree]
InterPro	IPR003329. Cytidylyl_trans. IPR004528. KdsB. [Graphical view]
Pfam	PF02348. CTP_transf_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00466. kdsB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSB_BRUA2

Accession

Primary (citable) accession number: Q2YPQ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 7, 2006
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)