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Protein

3-phosphoshikimate 1-carboxyvinyltransferase

Gene

aroA

Organism
Brucella abortus (strain 2308)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.UniRule annotation

Catalytic activityi

Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate.UniRule annotation

Pathway:ichorismate biosynthesis

This protein is involved in step 6 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroQ)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331Shikimate-3-phosphateUniRule annotation
Binding sitei128 – 1281PhosphoenolpyruvateUniRule annotation
Active sitei326 – 3261Proton acceptorUniRule annotation
Binding sitei353 – 3531Shikimate-3-phosphateUniRule annotation
Active sitei354 – 3541Proton donorUniRule annotation
Binding sitei357 – 3571PhosphoenolpyruvateUniRule annotation
Binding sitei402 – 4021PhosphoenolpyruvateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Enzyme and pathway databases

BioCyciBABO359391:GKDV-24-MONOMER.
BMEL359391:GJOQ-24-MONOMER.
UniPathwayiUPA00053; UER00089.

Names & Taxonomyi

Protein namesi
Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferaseUniRule annotation (EC:2.5.1.19UniRule annotation)
Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthaseUniRule annotation
Short name:
EPSP synthaseUniRule annotation
Short name:
EPSPSUniRule annotation
Gene namesi
Name:aroAUniRule annotation
Ordered Locus Names:BAB1_0023
OrganismiBrucella abortus (strain 2308)
Taxonomic identifieri359391 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000002719 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4504503-phosphoshikimate 1-carboxyvinyltransferasePRO_0000088232Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ2YPN6.
SMRiQ2YPN6. Positions 8-447.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni28 – 292Shikimate-3-phosphate bindingUniRule annotation
Regioni98 – 1014PhosphoenolpyruvateUniRule annotation

Sequence similaritiesi

Belongs to the EPSP synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0128.
HOGENOMiHOG000247371.
KOiK00800.
OMAiKRPMRRI.
OrthoDBiEOG6Z6FZ4.

Family and domain databases

Gene3Di3.65.10.10. 2 hits.
HAMAPiMF_00210. EPSP_synth.
InterProiIPR001986. Enolpyruvate_Tfrase_dom.
IPR006264. EPSP_synthase.
IPR023193. EPSP_synthase_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PfamiPF00275. EPSP_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000505. EPSPS. 1 hit.
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR01356. aroA. 1 hit.
PROSITEiPS00104. EPSP_SYNTHASE_1. 1 hit.
PS00885. EPSP_SYNTHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2YPN6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHSACPKPA TARHSQALTG EIRIPGDKSI SHRSFMFGGL ASGKTRITGL
60 70 80 90 100
LEGEDVINTG RAMQAMGARI RKEGDVWIIN GVGNGCLLQP EAPLDFGNAG
110 120 130 140 150
TGARLTMGLV GTYDMKTSFI GDASLSKRPM GRVLNPLREM GVQVEAAEGD
160 170 180 190 200
RMPLTLIGPR TANPIAYRVP MASAQVKSAV LLAGLNTPGV TTVIEPVMTR
210 220 230 240 250
DHTEKMLQGF GADLTVETDK DGVRHIRIVG QGKLTGQTID VPGDPSSTAF
260 270 280 290 300
PLVAALLVEG SEVTIRNVLM NPTRTGLILT LQEMGADIEI IDPRLAGGED
310 320 330 340 350
VADLRVKASK LKGVVVPPER APSMIDEYPV LAIAASFAEG ETVMDGLDEL
360 370 380 390 400
RVKESDRLAA VARGLEANGV DCTEGEMSLT VRGRPGGKGL GGGTVATHLD
410 420 430 440 450
HRIAMSFLVM GLASEKPVTV DDSTMIATSF PEFMGMMAGL GAKIAESGAE
Length:450
Mass (Da):47,235
Last modified:December 20, 2005 - v1
Checksum:iC6EEFD498CA72890
GO

Sequence cautioni

The sequence AAK27445.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti32 – 321H → T in AAK27445 (Ref. 1) Curated
Sequence conflicti254 – 2541A → P in AAK27445 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF326475 Genomic DNA. Translation: AAK27445.1. Different initiation.
AM040264 Genomic DNA. Translation: CAJ09979.1.
RefSeqiWP_002965272.1. NZ_KN046823.1.

Genome annotation databases

EnsemblBacteriaiCAJ09979; CAJ09979; BAB1_0023.
GeneIDi3788620.
KEGGibmf:BAB1_0023.
PATRICi17842863. VBIBruMel86222_0024.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF326475 Genomic DNA. Translation: AAK27445.1. Different initiation.
AM040264 Genomic DNA. Translation: CAJ09979.1.
RefSeqiWP_002965272.1. NZ_KN046823.1.

3D structure databases

ProteinModelPortaliQ2YPN6.
SMRiQ2YPN6. Positions 8-447.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ09979; CAJ09979; BAB1_0023.
GeneIDi3788620.
KEGGibmf:BAB1_0023.
PATRICi17842863. VBIBruMel86222_0024.

Phylogenomic databases

eggNOGiCOG0128.
HOGENOMiHOG000247371.
KOiK00800.
OMAiKRPMRRI.
OrthoDBiEOG6Z6FZ4.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00089.
BioCyciBABO359391:GKDV-24-MONOMER.
BMEL359391:GJOQ-24-MONOMER.

Family and domain databases

Gene3Di3.65.10.10. 2 hits.
HAMAPiMF_00210. EPSP_synth.
InterProiIPR001986. Enolpyruvate_Tfrase_dom.
IPR006264. EPSP_synthase.
IPR023193. EPSP_synthase_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PfamiPF00275. EPSP_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000505. EPSPS. 1 hit.
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR01356. aroA. 1 hit.
PROSITEiPS00104. EPSP_SYNTHASE_1. 1 hit.
PS00885. EPSP_SYNTHASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the aroA gene of Brucella abortus and construction of an aroA mutant."
    Gan T., Essenberg R.C.
    Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 2308.

Entry informationi

Entry nameiAROA_BRUA2
AccessioniPrimary (citable) accession number: Q2YPN6
Secondary accession number(s): Q57FY8, Q9AGV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: December 20, 2005
Last modified: July 22, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Brucella abortus strain 2308
    Brucella abortus (strain 2308): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.