Q2YPD3 (UREF1_BRUA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Urease accessory protein UreF 1

Gene names

Name:	ureF1
Ordered Locus Names:	BAB1_0302

Organism

Brucella abortus (strain 2308) [Complete proteome] [HAMAP]

Taxonomic identifier

359391 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	228 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for maturation of urease via the functional incorporation of the urease nickel metallocenter By similarity. Ref.1 Disruption of the ure1 gene cluster suggests that it protects brucellae during their passage through the stomach. The major route of infection in human brucellosis is oral. Ref.1
Subunit structure	UreD, UreF and UreG form a complex that acts as a GTP-hydrolysis-dependent molecular chaperone, activating the urease apoprotein by helping to assemble the nickel containing metallocenter of UreC. The UreE protein probably delivers the nickel By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_01385.
Sequence similarities	Belongs to the UreF family.

Ontologies

Keywords
Biological process	Nickel insertion Virulence
Cellular component	Cytoplasm
Molecular function	Chaperone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nitrogen compound metabolic process Inferred from electronic annotation. Source: InterPro pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nickel cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 228	228	Urease accessory protein UreF 1 HAMAP MF_01385		PRO_0000344085

Sequences

	Sequence		Length	Mass (Da)
	Q2YPD3 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 019E0C3CE1314F19	FASTA	228	24,450
	10 20 30 40 50 60 MTIITPITND PTTALLRLMA WLSPVFPVGS FSYSHGLERA VHDGLVVDAA GLQDWLQWLV 70 80 90 100 110 120 RRGSGWNDAV LCAESWRCAM KGEDLHEIAE LAEALAGSRE RHMETMLQGG AFFAAARSWP 130 140 150 160 170 180 CEIFDRLPPD CAYPVAVGAV AGGHGVPLAQ ALAAFLQAFC INLLQASIRL SVTGQSGVTA 190 200 210 220 IMAALEPVLG ETAARAALSS MEDLGSATFI ADIMAMKHET QHSRLFRS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the urease operon of Brucella abortus and assessment of its role in virulence of the bacterium." Sangari F.J., Seoane A., Rodriguez M.C., Aguero J., Garcia Lobo J.M. Infect. Immun. 75:774-780(2007) [PubMed: 17101645] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ROLE IN VIRULENCE.
[2]	"Whole-genome analyses of speciation events in pathogenic Brucellae." Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E. Infect. Immun. 73:8353-8361(2005) [PubMed: 16299333] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 2308.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF361941 Genomic DNA. Translation: AAK51071.1. AM040264 Genomic DNA. Translation: CAJ10258.1.
RefSeq	YP_413773.1. NC_007618.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2YPD3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3787080.
GenomeReviews	Gene locus BAB1_0302 in contig AM040264_GR.
KEGG	bmf:BAB1_0302.
PATRIC	17843472. VBIBruMel86222_0322.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0830.
HOGENOM	HBG709295.
OMA	WLSPAFP.
ProtClustDB	CLSK864150.
Enzyme and pathway databases
BioCyc	BMEL359391:BAB1_0302-MONOMER.
Family and domain databases
HAMAP	MF_01385. UreF. [Tree]
InterPro	IPR002639. UreF. [Graphical view]
KO	K03188.
Pfam	PF01730. UreF. 1 hit. [Graphical view]
PIRSF	PIRSF009467. Ureas_acces_UreF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UREF1_BRUA2

Accession

Primary (citable) accession number: Q2YPD3
Secondary accession number(s): Q57F83, Q93T79

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	February 7, 2006
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program