Q2YP58 (THIG_BRUA2) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 41.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Thiazole synthase EC=2.8.1.10 | ||||
| Gene names |
| ||||
| Organism | Brucella abortus (strain 2308) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 359391 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella |
Protein attributes
| Sequence length | 256 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S By similarity. HAMAP MF_00443 |
| Catalytic activity | 1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O. HAMAP MF_00443 |
| Pathway | Cofactor biosynthesis; thiamine diphosphate biosynthesis. HAMAP MF_00443 |
| Subunit structure | Homotetramer. Forms heterodimers with either ThiH or ThiS By similarity. |
| Subcellular location | Cytoplasm By similarity HAMAP MF_00443. |
| Sequence similarities | Belongs to the ThiG family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Thiamine biosynthesis |
| Cellular component | Cytoplasm |
| Ligand | Schiff base |
| Molecular function | Transferase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | lyase activity Inferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 256 | 256 | Thiazole synthase HAMAP MF_00443 | PRO_0000236333 | |||||
Regions | |||||||||
| Region | 184 – 185 | 2 | DXP binding By similarity | ||||||
| Region | 206 – 207 | 2 | DXP binding By similarity | ||||||
Sites | |||||||||
| Active site | 96 | 1 | Schiff-base intermediate with DXP By similarity | ||||||
| Binding site | 157 | 1 | DXP; via amide nitrogen By similarity | ||||||
Sequences
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References
| [1] | "Whole-genome analyses of speciation events in pathogenic Brucellae." Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E. Infect. Immun. 73:8353-8361(2005) [PubMed: 16299333] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 2308. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AM040264 Genomic DNA. Translation: CAJ10172.1. |
| RefSeq | YP_413698.1. NC_007618.1. |
3D structure databases | |
| ProteinModelPortal | Q2YP58. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q2YP58. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 3787004. |
| GenomeReviews | Gene locus BAB1_0216 in contig AM040264_GR. |
| KEGG | bmf:BAB1_0216. |
| PATRIC | 17843273. VBIBruMel86222_0226. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| eggNOG | COG2022. |
| HOGENOM | HBG296821. |
| OMA | TAGCFTA. |
| ProtClustDB | PRK00208. |
Enzyme and pathway databases | |
| BioCyc | BMEL359391:BAB1_0216-MONOMER. |
Family and domain databases | |
| HAMAP | MF_00443. ThiG. [Tree] |
| InterPro | IPR013785. Aldolase_TIM. IPR008867. ThiG. [Graphical view] |
| Gene3D | G3DSA:3.20.20.70. Aldolase_TIM. 1 hit. |
| KO | K03149. |
| Pfam | PF05690. ThiG. 1 hit. [Graphical view] |
| SUPFAM | SSF110399. ThiG. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | THIG_BRUA2 | ||||||||
| Accession | Primary (citable) accession number: Q2YP58 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| Brucella abortus strain 2308 Brucella abortus (strain 2308): entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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