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Protein

Thiazole synthase

Gene

thiG

Organism
Brucella abortus (strain 2308)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S.UniRule annotation

Catalytic activityi

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei96Schiff-base intermediate with DXPUniRule annotation1
Binding sitei157DXP; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiazole synthaseUniRule annotation (EC:2.8.1.10UniRule annotation)
Gene namesi
Name:thiGUniRule annotation
Ordered Locus Names:BAB1_0216
OrganismiBrucella abortus (strain 2308)
Taxonomic identifieri359391 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000002719 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002363331 – 256Thiazole synthaseAdd BLAST256

Interactioni

Subunit structurei

Homotetramer. Forms heterodimers with either ThiH or ThiS.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ2YP58.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni184 – 185DXP bindingUniRule annotation2
Regioni206 – 207DXP bindingUniRule annotation2

Sequence similaritiesi

Belongs to the ThiG family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000248049.
KOiK03149.
OMAiAQYPSPA.

Family and domain databases

CDDicd04728. ThiG. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00443. ThiG. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR008867. ThiG.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2YP58-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLEFYGKRFE SRLLLGTAQY PSPSILADAV RASLSRIVTV SLRRESGEAR
60 70 80 90 100
AGQDFWALIK ALGVAVLPNT AGCHTPREAI TTAHMAREVF GTNWIKLEVI
110 120 130 140 150
GDTDTLQPDP FGLVEAARIL CDEGFEVFPY MNDDLIVAER LIEAGCKVLM
160 170 180 190 200
PWGAPIGSGR GLNNPYALKT MRAHFPDIPL VVDAGIGVPS HAAAAMELGF
210 220 230 240 250
DAVLINTAVA KAGDPAAMAR AFALAVEAGR LAYEADPIEA RDMASPSTPL

LGKAFL
Length:256
Mass (Da):27,321
Last modified:December 20, 2005 - v1
Checksum:iB4BB05AAAF6E0510
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM040264 Genomic DNA. Translation: CAJ10172.1.
RefSeqiWP_002965462.1. NZ_KN046823.1.

Genome annotation databases

EnsemblBacteriaiCAJ10172; CAJ10172; BAB1_0216.
GeneIDi3787004.
KEGGibmf:BAB1_0216.
PATRICi17843273. VBIBruMel86222_0226.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM040264 Genomic DNA. Translation: CAJ10172.1.
RefSeqiWP_002965462.1. NZ_KN046823.1.

3D structure databases

ProteinModelPortaliQ2YP58.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ10172; CAJ10172; BAB1_0216.
GeneIDi3787004.
KEGGibmf:BAB1_0216.
PATRICi17843273. VBIBruMel86222_0226.

Phylogenomic databases

HOGENOMiHOG000248049.
KOiK03149.
OMAiAQYPSPA.

Enzyme and pathway databases

UniPathwayiUPA00060.

Family and domain databases

CDDicd04728. ThiG. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00443. ThiG. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR008867. ThiG.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTHIG_BRUA2
AccessioniPrimary (citable) accession number: Q2YP58
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Brucella abortus strain 2308
    Brucella abortus (strain 2308): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.