Q2YP58 (THIG_BRUA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thiazole synthase
EC=2.8.1.10

Gene names

Name:	thiG
Ordered Locus Names:	BAB1_0216

Organism

Brucella abortus (strain 2308) [Complete proteome] [HAMAP]

Taxonomic identifier

359391 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S By similarity. HAMAP MF_00443
Catalytic activity	1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O. HAMAP MF_00443
Pathway	Cofactor biosynthesis; thiamine diphosphate biosynthesis. HAMAP MF_00443
Subunit structure	Homotetramer. Forms heterodimers with either ThiH or ThiS By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_00443.
Sequence similarities	Belongs to the ThiG family.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	lyase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 256	256	Thiazole synthase HAMAP MF_00443		PRO_0000236333
Regions
	Region	184 – 185	2	DXP binding By similarity
	Region	206 – 207	2	DXP binding By similarity
Sites
	Active site	96	1	Schiff-base intermediate with DXP By similarity
	Binding site	157	1	DXP; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2YP58 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: B4BB05AAAF6E0510	FASTA	256	27,321
	10 20 30 40 50 60 MLEFYGKRFE SRLLLGTAQY PSPSILADAV RASLSRIVTV SLRRESGEAR AGQDFWALIK 70 80 90 100 110 120 ALGVAVLPNT AGCHTPREAI TTAHMAREVF GTNWIKLEVI GDTDTLQPDP FGLVEAARIL 130 140 150 160 170 180 CDEGFEVFPY MNDDLIVAER LIEAGCKVLM PWGAPIGSGR GLNNPYALKT MRAHFPDIPL 190 200 210 220 230 240 VVDAGIGVPS HAAAAMELGF DAVLINTAVA KAGDPAAMAR AFALAVEAGR LAYEADPIEA 250 RDMASPSTPL LGKAFL

References

[1]

"Whole-genome analyses of speciation events in pathogenic Brucellae." Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E. Infect. Immun. 73:8353-8361(2005) [PubMed: 16299333] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 2308.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM040264 Genomic DNA. Translation: CAJ10172.1.
RefSeq	YP_413698.1. NC_007618.1.
3D structure databases
ProteinModelPortal	Q2YP58.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2YP58.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3787004.
GenomeReviews	Gene locus BAB1_0216 in contig AM040264_GR.
KEGG	bmf:BAB1_0216.
PATRIC	17843273. VBIBruMel86222_0226.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2022.
HOGENOM	HBG296821.
OMA	TAGCFTA.
ProtClustDB	PRK00208.
Enzyme and pathway databases
BioCyc	BMEL359391:BAB1_0216-MONOMER.
Family and domain databases
HAMAP	MF_00443. ThiG. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR008867. ThiG. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K03149.
Pfam	PF05690. ThiG. 1 hit. [Graphical view]
SUPFAM	SSF110399. ThiG. 1 hit.
ProtoNet	Search...

Entry information

Entry name

THIG_BRUA2

Accession

Primary (citable) accession number: Q2YP58

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	December 20, 2005
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program