Q2YNW9 (ILVD_BRUA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	BAB1_0096

Organism

Brucella abortus (strain 2308) [Complete proteome] [HAMAP]

Taxonomic identifier

359391 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	611 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 611	611	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000000959
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	195	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q2YNW9 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: EB112A030C2D7831	FASTA	611	65,310
	10 20 30 40 50 60 MPPYRSRTTT HGRNMAGARG LWRATGMKDE DFGKPIIAVV NSFTQFVPGH VHLKDLGQLV 70 80 90 100 110 120 AREIESAGGV AKEFNTIAVD DGIAMGHDGM LYSLPSRELI ADSVEYMVNA HCADAMVCIS 130 140 150 160 170 180 NCDKITPGML MAALRLNIPV VFVSGGPMEA GKVVWEDSVK KLDLVDAMVA AADDHYTDEQ 190 200 210 220 230 240 VKAIERSACP TCGSCSGMFT ANSMNCLTEA LGLSLPGNGS TLATHADRKR LFVEAGHLIV 250 260 270 280 290 300 DLARRYYEQD DESVLPRSIA TFSAFENAMT LDIAMGGSTN TVLHLLAAAQ EAEIDFTMAD 310 320 330 340 350 360 IDRLSRRVPV LCKVAPAVSS VHMEDVHHAG GIMGILGQLD NAGLLTTSIP TVHSETLAKA 370 380 390 400 410 420 LDHWDVTRTN SEMVHKFYSA APGGVPTQVA FSQERRFDKV DTDREKGVIR SKEHAFSQDG 430 440 450 460 470 480 GLAVLYGNLA EDGCIVKTAG VDDSILKFSG PARIFESQDS AVLGILNGKI KPGDIVLIRY 490 500 510 520 530 540 EGPRGGPGMQ EMLYPTSYLK SKGLGKACAL ITDGRFSGGS SGLSIGHVSP EAAEGGTIGL 550 560 570 580 590 600 VREGDIIDID IPNRKIHLAV DDATLAERRA EQDAAGWKPA EERKRKISTA LKAYAAMATS 610 AARGAVRKLP D

References

[1]

"Whole-genome analyses of speciation events in pathogenic Brucellae." Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E. Infect. Immun. 73:8353-8361(2005) [PubMed: 16299333] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 2308.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM040264 Genomic DNA. Translation: CAJ10052.1.
RefSeq	YP_413592.1. NC_007618.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2YNW9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3788763.
GenomeReviews	Gene locus BAB1_0096 in contig AM040264_GR.
KEGG	bmf:BAB1_0096.
PATRIC	17843019. VBIBruMel86222_0101.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0129.
HOGENOM	HBG671001.
OMA	SQDCRWP.
ProtClustDB	PRK12448.
Enzyme and pathway databases
BioCyc	BMEL359391:BAB1_0096-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_BRUA2

Accession

Primary (citable) accession number: Q2YNW9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 7, 2006
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program