Reviewed, UniProtKB/Swiss-Prot Q2YNV3 (RLMN_BRUA2)

Names and origin

Protein names

Recommended name:
    Ribosomal RNA large subunit methyltransferase N
    EC=2.1.1.-
Alternative name(s):
    23S rRNA m2A2503 methyltransferase

Gene names

Name:	rlmN
Ordered Locus Names:	BAB1_0074

Organism

Brucella abortus (strain 2308) [Complete proteome] [HAMAP]

Taxonomic identifier

359391 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates position 2 of adenine 2503 in 23S rRNA By similarity.
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine Potential.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the radical SAM superfamily. RlmN family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW RNA methyltransferase activity Inferred from electronic annotation. Source: InterPro S-adenosylmethionine-dependent methyltransferase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 411	411	Ribosomal RNA large subunit methyltransferase N HAMAP MF_01849		PRO_0000350063
Sites
	Metal binding	145	1	Iron-sulfur (4Fe-4S-S-AdoMet) Potential
	Metal binding	149	1	Iron-sulfur (4Fe-4S-S-AdoMet) Potential
	Metal binding	152	1	Iron-sulfur (4Fe-4S-S-AdoMet) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q2YNV3-1 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 48D79A830A9A02BC	FASTA	411	45,813
	10 20 30 40 50 60 MSISFDLTID DTRDQLARHA RASLEAKPSL IGMSREEMAA ALIAAGVPER QVKMRISQLW 70 80 90 100 110 120 HWLYVRGVSD FADMRNISKD LRAMLAQHFT IARPEVVEEQ ISQDGTRKWL FRFPPRGAGR 130 140 150 160 170 180 PVEIESVYIP EEGRGTLCIS SQVGCTLTCS FCHTGTQKLV RNLTSEEILA QLLTARDRLG 190 200 210 220 230 240 DFPDKDTPDG AMVPAEGRKI TNIVMMGMGE PLYNFEEVKK ALLIASDGDG LSLSKCRITL 250 260 270 280 290 300 STSGVVPEIY RTGDEIGVML AISLHAVRDE LRDILVPINK KYPLAELIKA CREYPGLSNA 310 320 330 340 350 360 KRITFEYVML KDINDSLDDA KLLVKLLQGI PAKINLIPFN PWPGTNYQCS DWEQIEKFAD 370 380 390 400 410 YVNAAGYASP IRTPRGRDIL AACGQLKSES ERLRKSERLA LEAMMIAGHG E

References

[1]

"Whole-genome analyses of speciation events in pathogenic Brucellae." Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E. Infect. Immun. 73:8353-8361(2005) [PubMed: 16299333] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM040264 Genomic DNA. Translation: CAJ10030.1.
RefSeq	YP_413574.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2YNV3.
Genome annotation databases
GeneID	3786886.
GenomeReviews	Gene locus BAB1_0074 in contig AM040264_GR.
KEGG	bmf:BAB1_0074.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2YNV3.
OMA	TFCSTAQ
Enzyme and pathway databases
BioCyc	BMEL359391:BAB1_0074-MON.
Family and domain databases
HAMAP	MF_01849. [Tree]
InterPro	IPR006638. Elp3/MiaB/NifB. IPR007197. Radical_SAM. IPR004383. rRNA_lsu_MTrfase_RlmN. [Graphical view]
Pfam	PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF006004. CHP00048. 1 hit.
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00048. CHP48. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMN_BRUA2

Accession

Primary (citable) accession number: Q2YNV3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	February 7, 2006
Last modified:	November 24, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)