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Protein

GTP 3',8-cyclase

Gene

moaA

Organism
Brucella abortus (strain 2308)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate.UniRule annotation

Catalytic activityi

GTP + S-adenosyl-L-methionine + reduced electron acceptor = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + oxidized electron acceptor.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.UniRule annotation

Pathwayi: molybdopterin biosynthesis

This protein is involved in the pathway molybdopterin biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway molybdopterin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei28GTPUniRule annotation1
Metal bindingi35Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi39Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Binding sitei41S-adenosyl-L-methionineUniRule annotation1
Metal bindingi42Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Binding sitei77GTPUniRule annotation1
Binding sitei81S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei111GTPUniRule annotation1
Binding sitei135S-adenosyl-L-methionineUniRule annotation1
Binding sitei171GTPUniRule annotation1
Binding sitei205S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi268Iron-sulfur 2 (4Fe-4S-substrate)UniRule annotation1
Metal bindingi271Iron-sulfur 2 (4Fe-4S-substrate)UniRule annotation1
Metal bindingi285Iron-sulfur 2 (4Fe-4S-substrate)UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi273 – 275GTPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processMolybdenum cofactor biosynthesis
Ligand4Fe-4S, GTP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00344

Names & Taxonomyi

Protein namesi
Recommended name:
GTP 3',8-cyclaseUniRule annotation (EC:4.1.99.22UniRule annotation)
Alternative name(s):
Molybdenum cofactor biosynthesis protein AUniRule annotation
Gene namesi
Name:moaAUniRule annotation
Ordered Locus Names:BAB1_0973
OrganismiBrucella abortus (strain 2308)
Taxonomic identifieri359391 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000002719 Componenti: Chromosome I

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000541751 – 344GTP 3',8-cyclaseAdd BLAST344

Interactioni

Subunit structurei

Monomer and homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ2YNT7
SMRiQ2YNT7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. MoaA family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000228680
KOiK03639
OMAiIEFMPIG

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01225_B MoaA_B, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR034481 Main_SPASM_domain-containing
IPR013483 MoaA
IPR000385 MoaA_NifB_PqqE_Fe-S-bd_CS
IPR010505 Mob_synth_C
IPR007197 rSAM
IPR034395 SPASM/twitch_domain-containing
PfamiView protein in Pfam
PF06463 Mob_synth_C, 1 hit
PF04055 Radical_SAM, 1 hit
SFLDiSFLDG01067 SPASM/twitch_domain_containing, 1 hit
SFLDG01383 cyclic_pyranopterin_phosphate_, 1 hit
SFLDG01386 main_SPASM_domain-containing, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR02666 moaA, 1 hit
PROSITEiView protein in PROSITE
PS01305 MOAA_NIFB_PQQE, 1 hit

Sequencei

Sequence statusi: Complete.

Q2YNT7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRNVQDQPLV SPTEPMIDPF GRAVTYLRVS VTDRCDFRCT YCMAEHMTFL
60 70 80 90 100
PKKDLLTLEE LDRLCSVFIE KGVRKLRLTG GEPLVRKNIM HLIGNLSRHL
110 120 130 140 150
KSGALDELTL TTNGSQLARF AGELADCGVR RINVSLDTLN PEKFRTITRW
160 170 180 190 200
GDLSRVLEGI DAAQKAAIHV KINAVALKDF NDAEIPELIR WAHGRGMDVT
210 220 230 240 250
LIETMPMGEI EFDRTDQYLP LSQVRADLAS QFTLADIPYR TGGPARYVTI
260 270 280 290 300
SETGGRLGFI TPMTYNFCES CNRVRLTCTG MLYMCLGQND DADLRKALRE
310 320 330 340
SESDEHLSQA IDEAISRKPK GHDFIIDREH NRPSVARHMS LTGG
Length:344
Mass (Da):38,687
Last modified:February 7, 2006 - v1
Checksum:i50B8258570E51148
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM040264 Genomic DNA Translation: CAJ10929.1
RefSeqiWP_002966802.1, NZ_KN046823.1

Genome annotation databases

EnsemblBacteriaiCAJ10929; CAJ10929; BAB1_0973
KEGGibmf:BAB1_0973

Similar proteinsi

Entry informationi

Entry nameiMOAA_BRUA2
AccessioniPrimary (citable) accession number: Q2YNT7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 7, 2006
Last modified: April 25, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health