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Q2YNG6

- PURL_BRUA2

UniProt

Q2YNG6 - PURL_BRUA2

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Protein

Phosphoribosylformylglycinamidine synthase subunit PurL

Gene

purL

Organism
Brucella abortus (strain 2308)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.UniRule annotation

Catalytic activityi

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei50 – 501UniRule annotation
Binding sitei53 – 531ATPUniRule annotation
Binding sitei92 – 921ATPUniRule annotation
Metal bindingi94 – 941Magnesium 1UniRule annotation
Active sitei96 – 961Proton acceptorUniRule annotation
Binding sitei117 – 1171SubstrateUniRule annotation
Metal bindingi118 – 1181Magnesium 2UniRule annotation
Binding sitei241 – 2411SubstrateUniRule annotation
Metal bindingi269 – 2691Magnesium 2UniRule annotation
Binding sitei495 – 4951ATPUniRule annotation
Binding sitei532 – 5321ATP; via amide nitrogen and carbonyl oxygenUniRule annotation
Metal bindingi533 – 5331Magnesium 1UniRule annotation
Binding sitei535 – 5351SubstrateUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. phosphoribosylformylglycinamidine synthase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBABO359391:GKDV-871-MONOMER.
BMEL359391:GJOQ-871-MONOMER.
UniPathwayiUPA00074; UER00128.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine synthase subunit PurLUniRule annotation (EC:6.3.5.3UniRule annotation)
Short name:
FGAM synthaseUniRule annotation
Alternative name(s):
Formylglycinamide ribonucleotide amidotransferase subunit IIUniRule annotation
Short name:
FGAR amidotransferase IIUniRule annotation
Short name:
FGAR-AT IIUniRule annotation
Glutamine amidotransferase PurLUniRule annotation
Phosphoribosylformylglycinamidine synthase subunit IIUniRule annotation
Gene namesi
Name:purLUniRule annotation
Ordered Locus Names:BAB1_0857
OrganismiBrucella abortus (strain 2308)
Taxonomic identifieri359391 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000002719: Chromosome I

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 740740Phosphoribosylformylglycinamidine synthase subunit PurLPRO_0000236649Add
BLAST

Interactioni

Subunit structurei

Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.UniRule annotation

Protein-protein interaction databases

STRINGi359391.BAB1_0857.

Structurei

3D structure databases

ProteinModelPortaliQ2YNG6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni95 – 984Substrate bindingUniRule annotation
Regioni313 – 3153Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the FGAMS family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0046.
HOGENOMiHOG000238227.
KOiK01952.
OMAiQAVVFKI.
OrthoDBiEOG6FNHHR.

Family and domain databases

Gene3Di3.30.1330.10. 2 hits.
HAMAPiMF_00420. PurL_2.
InterProiIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR010074. PRibForGlyAmidine_synth_II.
IPR016188. PurM_N-like.
[Graphical view]
PfamiPF00586. AIRS. 2 hits.
PF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMiSSF55326. SSF55326. 2 hits.
SSF56042. SSF56042. 2 hits.
TIGRFAMsiTIGR01736. FGAM_synth_II. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2YNG6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTISNTRDIT PELIEAHGLK PDEYQRILEL IGREPTFTEL GIFSAMWNEH
60 70 80 90 100
CSYKSSKKWL RTLPTSGPRV IQGPGENAGV VDIGDGDCVV FKMESHNHPS
110 120 130 140 150
YIEPYQGAAT GVGGILRDVF TMGARPVAAM NALRFGEPDH PKTRHLVSGV
160 170 180 190 200
VSGVGGYGNA FGVPTVGGEV NFDKRYNGNI LVNAFAAGLA RHDGIFLSEA
210 220 230 240 250
EGVGLPVVYL GAKTSRDGVG GATMASAEFD ESIEEKRPTV QVGDPFTEKC
260 270 280 290 300
LLEACLELMA SGAVIAIQDM GAAGLTCSAV EMGAKGDLGI ELILDHVPVR
310 320 330 340 350
EENMTAYEMM LSESQERMLM VLKPEKEAEA QAIFRKWGLD FAIVGKTTDD
360 370 380 390 400
LRFRVIHQGE EVANLPIKDL GDEAPEYDRP WMEPGKHAPL PASNVPQVED
410 420 430 440 450
YSAALLKLIG SPDLSSRRWV YEQYDTLIQG NSLQVPGGDA GVIRVEGHET
460 470 480 490 500
KALAFSSDVT PRYCEADPFE GGKQAVAECW RNITATGAEP LASTDNLNFG
510 520 530 540 550
NPEKPEIMGQ LVKAIEGIGE ACRALDFPIV SGNVSLYNET NGQAILPTPT
560 570 580 590 600
IAGVGLLPDW SQMAKIGGMQ DGDTLVLLGG DGTHLGQSVY LRDLFDRADG
610 620 630 640 650
PAPFVDLALE KRNGEFVRSA IRNGQVTACH DLSDGGLAIA VAEMAIKSGK
660 670 680 690 700
GATLDAGDGL PHALLFGEDQ ARYVISATPE MAKLIALNAE GAGVPFRILG
710 720 730 740
TVGGDRLKIS KNVDVSVADL TQAYEGWFPN FMNGELTGNN
Length:740
Mass (Da):79,148
Last modified:February 7, 2006 - v1
Checksum:iC59E25D61D763D78
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM040264 Genomic DNA. Translation: CAJ10813.1.
RefSeqiYP_414285.1. NC_007618.1.

Genome annotation databases

EnsemblBacteriaiCAJ10813; CAJ10813; BAB1_0857.
GeneIDi3787541.
KEGGibmf:BAB1_0857.
PATRICi17844627. VBIBruMel86222_0892.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM040264 Genomic DNA. Translation: CAJ10813.1 .
RefSeqi YP_414285.1. NC_007618.1.

3D structure databases

ProteinModelPortali Q2YNG6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 359391.BAB1_0857.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAJ10813 ; CAJ10813 ; BAB1_0857 .
GeneIDi 3787541.
KEGGi bmf:BAB1_0857.
PATRICi 17844627. VBIBruMel86222_0892.

Phylogenomic databases

eggNOGi COG0046.
HOGENOMi HOG000238227.
KOi K01952.
OMAi QAVVFKI.
OrthoDBi EOG6FNHHR.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00128 .
BioCyci BABO359391:GKDV-871-MONOMER.
BMEL359391:GJOQ-871-MONOMER.

Miscellaneous databases

PROi Q2YNG6.

Family and domain databases

Gene3Di 3.30.1330.10. 2 hits.
HAMAPi MF_00420. PurL_2.
InterProi IPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR010074. PRibForGlyAmidine_synth_II.
IPR016188. PurM_N-like.
[Graphical view ]
Pfami PF00586. AIRS. 2 hits.
PF02769. AIRS_C. 2 hits.
[Graphical view ]
SUPFAMi SSF55326. SSF55326. 2 hits.
SSF56042. SSF56042. 2 hits.
TIGRFAMsi TIGR01736. FGAM_synth_II. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 2308.

Entry informationi

Entry nameiPURL_BRUA2
AccessioniPrimary (citable) accession number: Q2YNG6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: February 7, 2006
Last modified: October 29, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Brucella abortus strain 2308
    Brucella abortus (strain 2308): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3