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Q2YNE3 (SYP_BRUA2) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proline--tRNA ligase

EC=6.1.1.15
Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS
Gene names
Name:proS
Ordered Locus Names:BAB1_0842
OrganismBrucella abortus (strain 2308) [Complete proteome] [HAMAP]
Taxonomic identifier359391 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length442 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP-Rule MF_01570

Catalytic activity

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP-Rule MF_01570

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01570

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01570.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processprolyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

proline-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 442442Proline--tRNA ligase HAMAP-Rule MF_01570
PRO_0000248892

Sequences

Sequence LengthMass (Da)Tools
Q2YNE3 [UniParc].

Last modified February 7, 2006. Version 1.
Checksum: BCED4B480F6E7875

FASTA44249,507
        10         20         30         40         50         60 
MRLSRYFLPI LKENPKEAEI VSHRLMLRSG MIRQQSAGIY SWLPIGLKVL NKVCTIIREE 

        70         80         90        100        110        120 
QNRAGANEIL MPTIQSADLW RESGRYDAYG KEMLRIQDRQ KREMLFGPTN EEMVTDIFRS 

       130        140        150        160        170        180 
YVRSYKDLPL NLYHIQWKFR DEVRPRFGVM RSREFLMKDA YSFDLDYEGA KMAYYRMFVS 

       190        200        210        220        230        240 
YLRTFARVGL QAIPMRADTG PIGGDLSHEF IILAETGESQ VYCDRAYLDL AVPGADTDFR 

       250        260        270        280        290        300 
NDAQLTDIVT RWTTPYAATD EMHDEADWAK VKPESQVSAR GIEVGHIFHF GTKYSEPMGA 

       310        320        330        340        350        360 
KVQGPDGKEH LVSMGSYGIG PSRLVAAAIE ASHDDAGIIW PKTIAPFGAG IVNMKPGDEG 

       370        380        390        400        410        420 
CDGVSEKLYE ALTNAGVDPL LDDKDERPGA KFATMDLIGL PTQVIVGPRG VAAGEVEVKD 

       430        440 
RKTGERQSLG IKAAINMLTA QA 

« Hide

References

[1]"Whole-genome analyses of speciation events in pathogenic Brucellae."
Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E.
Infect. Immun. 73:8353-8361(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 2308.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM040264 Genomic DNA. Translation: CAJ10798.1.
RefSeqYP_414273.1. NC_007618.1.

3D structure databases

ProteinModelPortalQ2YNE3.
SMRQ2YNE3. Positions 1-439.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING359391.BAB1_0842.

Proteomic databases

PRIDEQ2YNE3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAJ10798; CAJ10798; BAB1_0842.
GeneID3787527.
KEGGbmf:BAB1_0842.
PATRIC17844597. VBIBruMel86222_0877.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0442.
HOGENOMHOG000076894.
KOK01881.
OMAIQPAELW.
OrthoDBEOG6TTVMR.
ProtClustDBPRK12325.

Enzyme and pathway databases

BioCycBABO359391:GKDV-856-MONOMER.
BMEL359391:GJOQ-856-MONOMER.

Family and domain databases

Gene3D3.40.50.800. 1 hit.
HAMAPMF_01570. Pro_tRNA_synth_type2.
InterProIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004500. Pro-tRNA-synth_IIa_bac-type.
IPR023716. Prolyl-tRNA_ligase_IIa_type2.
[Graphical view]
PANTHERPTHR11451:SF3. PTHR11451:SF3. 1 hit.
PfamPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PRINTSPR01046. TRNASYNTHPRO.
SUPFAMSSF52954. SSF52954. 1 hit.
TIGRFAMsTIGR00409. proS_fam_II. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYP_BRUA2
AccessionPrimary (citable) accession number: Q2YNE3
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: February 7, 2006
Last modified: February 19, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Brucella abortus strain 2308

Brucella abortus (strain 2308): entries and gene names

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries