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Q2YNA7

- SYDND_BRUA2

UniProt

Q2YNA7 - SYDND_BRUA2

Protein

Aspartate--tRNA(Asp/Asn) ligase

Gene

aspS

Organism
Brucella abortus (strain 2308)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (07 Feb 2006)
      Previous versions | rss
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    Functioni

    Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).UniRule annotation

    Catalytic activityi

    ATP + L-aspartate + tRNA(Asx) = AMP + diphosphate + L-aspartyl-tRNA(Asx).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei33 – 331Important for tRNA non-discriminationUniRule annotation
    Binding sitei175 – 1751AspartateUniRule annotation
    Binding sitei221 – 2211AspartateUniRule annotation
    Binding sitei454 – 4541AspartateUniRule annotation
    Binding sitei488 – 4881ATPUniRule annotation
    Binding sitei491 – 4911AspartateUniRule annotation
    Binding sitei495 – 4951AspartateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi221 – 2233ATPUniRule annotation
    Nucleotide bindingi540 – 5434ATPUniRule annotation

    GO - Molecular functioni

    1. aspartate-tRNA(Asn) ligase activity Source: UniProtKB-EC
    2. aspartate-tRNA ligase activity Source: UniProtKB-HAMAP
    3. ATP binding Source: UniProtKB-HAMAP
    4. nucleic acid binding Source: InterPro

    GO - Biological processi

    1. aspartyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBABO359391:GKDV-786-MONOMER.
    BMEL359391:GJOQ-786-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aspartate--tRNA(Asp/Asn) ligaseUniRule annotation (EC:6.1.1.23UniRule annotation)
    Alternative name(s):
    Aspartyl-tRNA synthetaseUniRule annotation
    Short name:
    AspRSUniRule annotation
    Non-discriminating aspartyl-tRNA synthetaseUniRule annotation
    Short name:
    ND-AspRSUniRule annotation
    Gene namesi
    Name:aspSUniRule annotation
    Ordered Locus Names:BAB1_0775
    OrganismiBrucella abortus (strain 2308)
    Taxonomic identifieri359391 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
    ProteomesiUP000002719: Chromosome I

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 595595Aspartate--tRNA(Asp/Asn) ligasePRO_0000235512Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi359391.BAB1_0775.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2YNA7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni199 – 2024AspartateUniRule annotation

    Sequence similaritiesi

    Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0173.
    HOGENOMiHOG000275160.
    KOiK01876.
    OMAiSLSRKQI.
    OrthoDBiEOG68Q0NX.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    3.30.1360.30. 1 hit.
    HAMAPiMF_00044. Asp_tRNA_synth.
    InterProiIPR004364. aa-tRNA-synt_II.
    IPR018150. aa-tRNA-synt_II-like.
    IPR006195. aa-tRNA-synth_II.
    IPR004524. Asp-tRNA-ligase_IIb_bac/mt.
    IPR002312. Asp/Asn-tRNA-synth_IIb.
    IPR004115. GAD-like.
    IPR029351. GAD_dom.
    IPR012340. NA-bd_OB-fold.
    IPR004365. NA-bd_OB_tRNA.
    [Graphical view]
    PANTHERiPTHR22594. PTHR22594. 1 hit.
    PTHR22594:SF5. PTHR22594:SF5. 1 hit.
    PfamiPF02938. GAD. 1 hit.
    PF00152. tRNA-synt_2. 1 hit.
    PF01336. tRNA_anti-codon. 1 hit.
    [Graphical view]
    PRINTSiPR01042. TRNASYNTHASP.
    SUPFAMiSSF50249. SSF50249. 1 hit.
    SSF55261. SSF55261. 1 hit.
    TIGRFAMsiTIGR00459. aspS_bact. 1 hit.
    PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q2YNA7-1 [UniParc]FASTAAdd to Basket

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    MHRYRSHTCA ALRKTDVGSN VRLSGWVHRV RDHGGILFID LRDHYGITQI    50
    VADPDSPAFK VAETVRGEWV IRVDGEVKAR ADDAVNTNLP TGEVEIFATE 100
    IEVLSPAKEL PLPVFGEPDY PEDIRLKYRF LDLRRETLHK NIMSRTKIIA 150
    AMRRRMTEIG FNEFSTPILT ASSPEGARDF LVPSRIHPGK FYALPQAPQQ 200
    YKQLLMVAGF DRYFQIAPCF RDEDPRADRL PGEFYQLDLE MSFVTQEEVW 250
    ETMEPVMRGI FEEFAEGKPV TKVFRRIAYD DAIRTYGSDK PDLRNPIEMQ 300
    AVTDHFAGSG FKVFANMIAN DAKVEVWAIP AKTGGSRAFC DRMNSWAQSE 350
    GQPGLGYIFW RKEGDKLEGA GPIAKNIGEE RTEAIRKQMG LEDGDACFFV 400
    AGLPSKFYKF AGDARTRAGE ELNLVDRDRF ELAWIIDFPF YEWDEDNKKI 450
    DFAHNPFSLP QGGMDALENM DPLEIKAYQY DLVCNGFEIA SGSIRNQLPE 500
    VMVKAFEKVG LSQQDVEERF GGLYRAFQYG APPHGGMAAG IDRVIMLLVG 550
    AKNLREISLF PMNQQALDLL MGAPSEVSPA QLRDLHVRLA PVQKS 595
    Length:595
    Mass (Da):67,255
    Last modified:February 7, 2006 - v1
    Checksum:i6CAE4EDFF3F69E50
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM040264 Genomic DNA. Translation: CAJ10731.1.
    RefSeqiYP_414208.1. NC_007618.1.

    Genome annotation databases

    EnsemblBacteriaiCAJ10731; CAJ10731; BAB1_0775.
    GeneIDi3788627.
    KEGGibmf:BAB1_0775.
    PATRICi17844455. VBIBruMel86222_0809.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM040264 Genomic DNA. Translation: CAJ10731.1 .
    RefSeqi YP_414208.1. NC_007618.1.

    3D structure databases

    ProteinModelPortali Q2YNA7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 359391.BAB1_0775.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAJ10731 ; CAJ10731 ; BAB1_0775 .
    GeneIDi 3788627.
    KEGGi bmf:BAB1_0775.
    PATRICi 17844455. VBIBruMel86222_0809.

    Phylogenomic databases

    eggNOGi COG0173.
    HOGENOMi HOG000275160.
    KOi K01876.
    OMAi SLSRKQI.
    OrthoDBi EOG68Q0NX.

    Enzyme and pathway databases

    BioCyci BABO359391:GKDV-786-MONOMER.
    BMEL359391:GJOQ-786-MONOMER.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    3.30.1360.30. 1 hit.
    HAMAPi MF_00044. Asp_tRNA_synth.
    InterProi IPR004364. aa-tRNA-synt_II.
    IPR018150. aa-tRNA-synt_II-like.
    IPR006195. aa-tRNA-synth_II.
    IPR004524. Asp-tRNA-ligase_IIb_bac/mt.
    IPR002312. Asp/Asn-tRNA-synth_IIb.
    IPR004115. GAD-like.
    IPR029351. GAD_dom.
    IPR012340. NA-bd_OB-fold.
    IPR004365. NA-bd_OB_tRNA.
    [Graphical view ]
    PANTHERi PTHR22594. PTHR22594. 1 hit.
    PTHR22594:SF5. PTHR22594:SF5. 1 hit.
    Pfami PF02938. GAD. 1 hit.
    PF00152. tRNA-synt_2. 1 hit.
    PF01336. tRNA_anti-codon. 1 hit.
    [Graphical view ]
    PRINTSi PR01042. TRNASYNTHASP.
    SUPFAMi SSF50249. SSF50249. 1 hit.
    SSF55261. SSF55261. 1 hit.
    TIGRFAMsi TIGR00459. aspS_bact. 1 hit.
    PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 2308.

    Entry informationi

    Entry nameiSYDND_BRUA2
    AccessioniPrimary (citable) accession number: Q2YNA7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 16, 2006
    Last sequence update: February 7, 2006
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Brucella abortus strain 2308
      Brucella abortus (strain 2308): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3