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Q2YNA7

- SYDND_BRUA2

UniProt

Q2YNA7 - SYDND_BRUA2

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Protein

Aspartate--tRNA(Asp/Asn) ligase

Gene

aspS

Organism
Brucella abortus (strain 2308)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).UniRule annotation

Catalytic activityi

ATP + L-aspartate + tRNA(Asx) = AMP + diphosphate + L-aspartyl-tRNA(Asx).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei33 – 331Important for tRNA non-discriminationUniRule annotation
Binding sitei175 – 1751AspartateUniRule annotation
Binding sitei221 – 2211AspartateUniRule annotation
Binding sitei454 – 4541AspartateUniRule annotation
Binding sitei488 – 4881ATPUniRule annotation
Binding sitei491 – 4911AspartateUniRule annotation
Binding sitei495 – 4951AspartateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi221 – 2233ATPUniRule annotation
Nucleotide bindingi540 – 5434ATPUniRule annotation

GO - Molecular functioni

  1. aspartate-tRNA(Asn) ligase activity Source: UniProtKB-EC
  2. aspartate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. ATP binding Source: UniProtKB-HAMAP
  4. nucleic acid binding Source: InterPro

GO - Biological processi

  1. aspartyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBABO359391:GKDV-786-MONOMER.
BMEL359391:GJOQ-786-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate--tRNA(Asp/Asn) ligaseUniRule annotation (EC:6.1.1.23UniRule annotation)
Alternative name(s):
Aspartyl-tRNA synthetaseUniRule annotation
Short name:
AspRSUniRule annotation
Non-discriminating aspartyl-tRNA synthetaseUniRule annotation
Short name:
ND-AspRSUniRule annotation
Gene namesi
Name:aspSUniRule annotation
Ordered Locus Names:BAB1_0775
OrganismiBrucella abortus (strain 2308)
Taxonomic identifieri359391 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000002719: Chromosome I

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 595595Aspartate--tRNA(Asp/Asn) ligasePRO_0000235512Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi359391.BAB1_0775.

Structurei

3D structure databases

ProteinModelPortaliQ2YNA7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni199 – 2024AspartateUniRule annotation

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0173.
HOGENOMiHOG000275160.
KOiK01876.
OMAiSLSRKQI.
OrthoDBiEOG68Q0NX.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.30.1360.30. 1 hit.
HAMAPiMF_00044. Asp_tRNA_synth.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004524. Asp-tRNA-ligase_IIb_bac/mt.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR004115. GAD-like.
IPR029351. GAD_dom.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF5. PTHR22594:SF5. 1 hit.
PfamiPF02938. GAD. 1 hit.
PF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF55261. SSF55261. 1 hit.
TIGRFAMsiTIGR00459. aspS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2YNA7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MHRYRSHTCA ALRKTDVGSN VRLSGWVHRV RDHGGILFID LRDHYGITQI
60 70 80 90 100
VADPDSPAFK VAETVRGEWV IRVDGEVKAR ADDAVNTNLP TGEVEIFATE
110 120 130 140 150
IEVLSPAKEL PLPVFGEPDY PEDIRLKYRF LDLRRETLHK NIMSRTKIIA
160 170 180 190 200
AMRRRMTEIG FNEFSTPILT ASSPEGARDF LVPSRIHPGK FYALPQAPQQ
210 220 230 240 250
YKQLLMVAGF DRYFQIAPCF RDEDPRADRL PGEFYQLDLE MSFVTQEEVW
260 270 280 290 300
ETMEPVMRGI FEEFAEGKPV TKVFRRIAYD DAIRTYGSDK PDLRNPIEMQ
310 320 330 340 350
AVTDHFAGSG FKVFANMIAN DAKVEVWAIP AKTGGSRAFC DRMNSWAQSE
360 370 380 390 400
GQPGLGYIFW RKEGDKLEGA GPIAKNIGEE RTEAIRKQMG LEDGDACFFV
410 420 430 440 450
AGLPSKFYKF AGDARTRAGE ELNLVDRDRF ELAWIIDFPF YEWDEDNKKI
460 470 480 490 500
DFAHNPFSLP QGGMDALENM DPLEIKAYQY DLVCNGFEIA SGSIRNQLPE
510 520 530 540 550
VMVKAFEKVG LSQQDVEERF GGLYRAFQYG APPHGGMAAG IDRVIMLLVG
560 570 580 590
AKNLREISLF PMNQQALDLL MGAPSEVSPA QLRDLHVRLA PVQKS
Length:595
Mass (Da):67,255
Last modified:February 7, 2006 - v1
Checksum:i6CAE4EDFF3F69E50
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM040264 Genomic DNA. Translation: CAJ10731.1.
RefSeqiYP_414208.1. NC_007618.1.

Genome annotation databases

EnsemblBacteriaiCAJ10731; CAJ10731; BAB1_0775.
GeneIDi3788627.
KEGGibmf:BAB1_0775.
PATRICi17844455. VBIBruMel86222_0809.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM040264 Genomic DNA. Translation: CAJ10731.1 .
RefSeqi YP_414208.1. NC_007618.1.

3D structure databases

ProteinModelPortali Q2YNA7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 359391.BAB1_0775.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAJ10731 ; CAJ10731 ; BAB1_0775 .
GeneIDi 3788627.
KEGGi bmf:BAB1_0775.
PATRICi 17844455. VBIBruMel86222_0809.

Phylogenomic databases

eggNOGi COG0173.
HOGENOMi HOG000275160.
KOi K01876.
OMAi SLSRKQI.
OrthoDBi EOG68Q0NX.

Enzyme and pathway databases

BioCyci BABO359391:GKDV-786-MONOMER.
BMEL359391:GJOQ-786-MONOMER.

Family and domain databases

Gene3Di 2.40.50.140. 1 hit.
3.30.1360.30. 1 hit.
HAMAPi MF_00044. Asp_tRNA_synth.
InterProi IPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004524. Asp-tRNA-ligase_IIb_bac/mt.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR004115. GAD-like.
IPR029351. GAD_dom.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view ]
PANTHERi PTHR22594. PTHR22594. 1 hit.
PTHR22594:SF5. PTHR22594:SF5. 1 hit.
Pfami PF02938. GAD. 1 hit.
PF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view ]
PRINTSi PR01042. TRNASYNTHASP.
SUPFAMi SSF50249. SSF50249. 1 hit.
SSF55261. SSF55261. 1 hit.
TIGRFAMsi TIGR00459. aspS_bact. 1 hit.
PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 2308.

Entry informationi

Entry nameiSYDND_BRUA2
AccessioniPrimary (citable) accession number: Q2YNA7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: February 7, 2006
Last modified: October 29, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Brucella abortus strain 2308
    Brucella abortus (strain 2308): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3