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Protein

Cysteine--tRNA ligase

Gene

cysS

Organism
Brucella abortus (strain 2308)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi34 – 341ZincUniRule annotation
Metal bindingi230 – 2301ZincUniRule annotation
Metal bindingi269 – 2691ZincUniRule annotation
Metal bindingi273 – 2731ZincUniRule annotation
Binding sitei305 – 3051ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. cysteine-tRNA ligase activity Source: UniProtKB-HAMAP
  3. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. cysteinyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBABO359391:GKDV-706-MONOMER.
BMEL359391:GJOQ-706-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine--tRNA ligaseUniRule annotation (EC:6.1.1.16UniRule annotation)
Alternative name(s):
Cysteinyl-tRNA synthetaseUniRule annotation
Short name:
CysRSUniRule annotation
Gene namesi
Name:cysSUniRule annotation
Ordered Locus Names:BAB1_0697
OrganismiBrucella abortus (strain 2308)
Taxonomic identifieri359391 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000002719 Componenti: Chromosome I

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 506506Cysteine--tRNA ligasePRO_0000240894Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi359391.BAB1_0697.

Structurei

3D structure databases

ProteinModelPortaliQ2YN22.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi36 – 4611"HIGH" regionAdd
BLAST
Motifi302 – 3065"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0215.
HOGENOMiHOG000245250.
KOiK01883.
OMAiESGYDRE.
OrthoDBiEOG6RVFXC.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00041. Cys_tRNA_synth.
InterProiIPR015803. Cys-tRNA-ligase.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00435. cysS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2YN22-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPDTTPQLRL YNTLTRTKEA FAPIDAKNVR MYVCGPTVYD FAHIGNARPV
60 70 80 90 100
IVFDVLFRLL RHVYGAQHVT YARNITDVDD KINARAARDY PDLPFNEAIR
110 120 130 140 150
KVTESTNAQF QADVTALGNL QPTVQPRATE HMDEMRAMID RLVQRGVAYV
160 170 180 190 200
AQDHVLFSPS AMNARKGPRY GALARRSLDE MLAGARVDVA SYKRDEMDFV
210 220 230 240 250
LWKPSKKGEP GWPSPAGIET LGRPGWHIEC SAMSMAKLLE PFGGGLKCDD
260 270 280 290 300
PERNQFDIHG GGIDLVFPHH ENEIAQSCCA LGTERMANIW MHNGFLQVEG
310 320 330 340 350
QKMSKSLGNF ITIRDVLNDG LPQLGEWGDN TVRDHWAGLA ARLSMLQTHY
360 370 380 390 400
REPINWTAQR LAESADELHR WYGLLRDEGF GAPEKLSHAS AVAAALCDDL
410 420 430 440 450
NSWAAITALR QAFKVRDVAA LGEGMALMGL LDPYFVTASD VPIFARADVD
460 470 480 490 500
ASAIAARIAE RLNFINAKNW AEADRIRDEL LQEGVQLKDS KDPATGERIT

TWDVVG
Length:506
Mass (Da):56,284
Last modified:February 7, 2006 - v1
Checksum:i5B1DB5A1554DA639
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM040264 Genomic DNA. Translation: CAJ10653.1.
RefSeqiYP_414139.1. NC_007618.1.

Genome annotation databases

EnsemblBacteriaiCAJ10653; CAJ10653; BAB1_0697.
KEGGibmf:BAB1_0697.
PATRICi17844286. VBIBruMel86222_0726.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM040264 Genomic DNA. Translation: CAJ10653.1.
RefSeqiYP_414139.1. NC_007618.1.

3D structure databases

ProteinModelPortaliQ2YN22.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi359391.BAB1_0697.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ10653; CAJ10653; BAB1_0697.
KEGGibmf:BAB1_0697.
PATRICi17844286. VBIBruMel86222_0726.

Phylogenomic databases

eggNOGiCOG0215.
HOGENOMiHOG000245250.
KOiK01883.
OMAiESGYDRE.
OrthoDBiEOG6RVFXC.

Enzyme and pathway databases

BioCyciBABO359391:GKDV-706-MONOMER.
BMEL359391:GJOQ-706-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00041. Cys_tRNA_synth.
InterProiIPR015803. Cys-tRNA-ligase.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00435. cysS. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 2308.

Entry informationi

Entry nameiSYC_BRUA2
AccessioniPrimary (citable) accession number: Q2YN22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: February 7, 2006
Last modified: April 1, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Brucella abortus strain 2308
    Brucella abortus (strain 2308): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.