Q2YN11 (RSH_BRUA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTP pyrophosphokinase rsh
EC=2.7.6.5

Alternative name(s):
(p)ppGpp synthase
ATP:GTP 3'-pyrophosphotransferase

Gene names

Name:	rsh
Ordered Locus Names:	BAB1_0672

Organism

Brucella abortus (strain 2308) [Complete proteome] [HAMAP]

Taxonomic identifier

359391 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	750 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Functions as a (p)ppGpp synthase. In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. Plays a role in adaptation of Brucella to its intracellular host environment By similarity.
Catalytic activity	ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate.
Sequence similarities	Belongs to the relA/spoT family. Contains 1 ACT domain. Contains 1 HD domain.

Ontologies

Keywords
Ligand	ATP-binding GTP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	guanosine tetraphosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP diphosphokinase activity Inferred from electronic annotation. Source: EC amino acid binding Inferred from electronic annotation. Source: InterPro kinase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: InterPro phosphoric diester hydrolase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 750	750	GTP pyrophosphokinase rsh		PRO_0000322562
Regions
	Domain	45 – 144	100	HD
	Domain	675 – 738	64	ACT

Sequences

	Sequence		Length	Mass (Da)
	Q2YN11 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 4836EEECB0E52495	FASTA	750	83,856
	10 20 30 40 50 60 MMRQYELVER VQRYKPDVNE ALLNKAYVYA MQKHGSQKRA SGDPYFSHPL EVAAILTDMH 70 80 90 100 110 120 LDEATIAIAL LHDTIEDTTA TRQEIDQLFG PEIGKLVEGL TKLKKLDLVS KKAVQAENLR 130 140 150 160 170 180 KLLLAISEDV RVLLVKLADR LHNMRTLGVM REDKRLRIAE ETMDIYAPLA GRMGMQDMRE 190 200 210 220 230 240 ELEELAFRYI NPDAWRAVTD RLAELLEKNR GLLQKIETDL SEIFEKNGIK ASVKSRQKKP 250 260 270 280 290 300 WSVFRKMETK GLSFEQLSDI FGFRVMVDTV QDCYRALGLI HTTWSMVPGR FKDYISTPKQ 310 320 330 340 350 360 NDYRSIHTTI IGPSRQRIEL QIRTREMDEI AEFGVAAHSI YKDRGSANNP HKISTETNAY 370 380 390 400 410 420 AWLRQTIEQL SEGDNPEEFL EHTKLELFQD QVFCFTPKGR LIALPRGATP IDFAYAVHTD 430 440 450 460 470 480 IGDSCVGAKV NGRIMPLMTE LKNGDEVDII RSKAQVPPAA WESLVATGKA RAAIRRATRS 490 500 510 520 530 540 AVRKQYSGLG MRILERAFER AGKPFSKDIL KPGLPRLARK DVEDVLAAVG RGELPSADVV 550 560 570 580 590 600 KAVYPDYQDT RVTTQNNPAK AGEKGWFNIQ NAAGMIFKVP EGGEGAAAKV DPAATTPKPG 610 620 630 640 650 660 KRALPIRGTN PDLPVRFAPE GAVPGDRIVG ILQPGAGITI YPIQSPALTA YDDQPERWID 670 680 690 700 710 720 VRWDIDDQMS ERFPARISVS AINSPGSLAE IAQIAAANDA NIHNLSMART APDFTEMIID 730 740 750 VEVWDLKHLN RIISQLKESA SVSSAKRVNG

References

[1]

"Whole-genome analyses of speciation events in pathogenic Brucellae." Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E. Infect. Immun. 73:8353-8361(2005) [PubMed: 16299333] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 2308.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM040264 Genomic DNA. Translation: CAJ10628.1.
RefSeq	YP_414114.1. NC_007618.1.
3D structure databases
ProteinModelPortal	Q2YN11.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2YN11.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3787392.
GenomeReviews	Gene locus BAB1_0672 in contig AM040264_GR.
KEGG	bmf:BAB1_0672.
PATRIC	17844238. VBIBruMel86222_0702.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0317.
HOGENOM	HBG594146.
OMA	ADIGPIR.
ProtClustDB	CLSK2754108.
Enzyme and pathway databases
BioCyc	BMEL359391:BAB1_0672-MONOMER.
Family and domain databases
InterPro	IPR002912. ACT-bd. IPR012675. Beta-grasp_ferredoxin-type. IPR003607. Metal-dep_PHydrolase_HD_dom. IPR006674. Metal-dep_PHydrolase_HD_sub. IPR007685. RelA_SpoT. IPR004811. SpoT_RelA. IPR004095. TGS. IPR012676. TGS-like. [Graphical view]
Gene3D	G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
KO	K01139.
PANTHER	PTHR21262. PTHR21262. 1 hit.
Pfam	PF01842. ACT. 1 hit. PF01966. HD. 1 hit. PF04607. RelA_SpoT. 1 hit. PF02824. TGS. 1 hit. [Graphical view]
SMART	SM00471. HDc. 1 hit. SM00954. RelA_SpoT. 1 hit. [Graphical view]
SUPFAM	SSF81271. TGS-like. 1 hit.
TIGRFAMs	TIGR00691. SpoT_relA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSH_BRUA2

Accession

Primary (citable) accession number: Q2YN11

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	February 7, 2006
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program