Reviewed, UniProtKB/Swiss-Prot Q2YN02 (RNC_BRUA2)

Names and origin

Protein names

Recommended name:
    Ribonuclease 3
    EC=3.1.26.3
Alternative name(s):
    Ribonuclease III
      Short name=RNase III

Gene names

Name:	rnc
Ordered Locus Names:	BAB1_0681

Organism

Brucella abortus (strain 2308) [Complete proteome] [HAMAP]

Taxonomic identifier

359391 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Digests double-stranded RNA. Involved in the processing of ribosomal RNA precursors and of some mRNAs By similarity. HAMAP MF_00104
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP MF_00104
Subcellular location	Cytoplasm By similarity HAMAP MF_00104.
Sequence similarities	Contains 1 DRBM (double-stranded RNA-binding) domain. Contains 1 RNase III domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	mRNA processing Inferred from electronic annotation. Source: HAMAP rRNA catabolic process Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	double-stranded RNA binding Inferred from electronic annotation. Source: InterPro ribonuclease III activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	Ribonuclease 3 HAMAP MF_00104		PRO_0000228510
Regions
	Domain	19 – 144	126	RNase III
	Domain	169 – 238	70	DRBM

Sequences

	Sequence		Length	Mass (Da)
	Q2YN02-1 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 084ED2D3A3F16BFC	FASTA	245	27,220
	10 20 30 40 50 60 MNRTRPLPEI KMVSANKTAS ILEERTGHRF LNLKRLERAL THSSVQAPAR ANYERLEFLG 70 80 90 100 110 120 DRVLGLTVAE MLFEAFPEAS EGELSVRLNA LVNAETCAAI ADEIGLADLI HTGSDIKSLN 130 140 150 160 170 180 DKRLLNVRAD VVEALIATIY LDGGLEAARS FIQRYWKKRS LETGAARRDA KTELQEWAHQ 190 200 210 220 230 240 QGNVHPVYAI LSRSGPDHDP LFLVEVTVKG FAPEKGEGRS KRIAEQSAAE AMLYREGVWK RDGSA

References

[1]

"Whole-genome analyses of speciation events in pathogenic Brucellae." Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E. Infect. Immun. 73:8353-8361(2005) [PubMed: 16299333] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM040264 Genomic DNA. Translation: CAJ10637.1.
RefSeq	YP_414123.1.
3D structure databases
SMR	Q2YN02. Positions 19-234.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2YN02.
Genome annotation databases
GeneID	3788905.
GenomeReviews	Gene locus BAB1_0681 in contig AM040264_GR.
KEGG	bmf:BAB1_0681.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0571.
HOGENOM	HBG744556.
OMA	ALTHKSF.
Enzyme and pathway databases
BioCyc	BMEL359391:BAB1_0681-MONOMER.
Family and domain databases
HAMAP	MF_00104. RNase_III. [Tree]
InterPro	IPR001159. Ds-RNA_bd. IPR014720. dsRNA-bd-like. IPR000999. RNase_III. IPR011907. RNase_III_bac. [Graphical view]
Gene3D	G3DSA:3.30.160.20. dsRNA-bd-like. 1 hit. G3DSA:1.10.1520.10. RNase_III. 1 hit.
Pfam	PF00035. dsrm. 1 hit. PF00636. Ribonuclease_3. 1 hit. [Graphical view]
SMART	SM00358. DSRM. 1 hit. SM00535. RIBOc. 1 hit. [Graphical view]
TIGRFAMs	TIGR02191. RNaseIII. 1 hit.
PROSITE	PS50137. DS_RBD. 1 hit. PS00517. RNASE_3_1. 1 hit. PS50142. RNASE_3_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNC_BRUA2

Accession

Primary (citable) accession number: Q2YN02

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	February 7, 2006
Last modified:	February 9, 2010
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)