Reviewed, UniProtKB/Swiss-Prot Q2YMU7 (CCME_BRUA2)

Names and origin

Protein names

Recommended name:
    Cytochrome c-type biogenesis protein ccmE
Alternative name(s):
    Cytochrome c maturation protein E
    Heme chaperone ccmE

Gene names

Name:	ccmE
Synonyms:	cycJ
Ordered Locus Names:	BAB1_0632

Organism

Brucella abortus (strain 2308) [Complete proteome] [HAMAP]

Taxonomic identifier

359391 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	165 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Heme chaperone required for the biogenesis of c-type cytochromes. Transiently binds heme delivered by ccmC and transfers the heme to apo-cytochromes in a process facilitated by ccmF and ccmH By similarity.
Subcellular location	Cell inner membrane; Single-pass type II membrane protein; Periplasmic side Potential.
Sequence similarities	Belongs to the ccmE/cycJ family.

Ontologies

Keywords
Biological process	Cytochrome c-type biogenesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Signal-anchor Transmembrane
Ligand	Heme Iron Metal-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cytochrome complex assembly Inferred from electronic annotation. Source: UniProtKB-KW protein-heme linkage Inferred from electronic annotation. Source: HAMAP
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 165	165	Cytochrome c-type biogenesis protein ccmE HAMAP MF_01959		PRO_0000238799
Regions
	Topological domain	1 – 29	29	Cytoplasmic Potential
	Transmembrane	30 – 50	21	Signal-anchor for type II membrane protein Potential
	Topological domain	51 – 165	115	Periplasmic Potential
Sites
	Metal binding	147	1	Iron (heme axial ligand) By similarity
	Binding site	143	1	Heme (covalent; via pros nitrogen) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2YMU7-1 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: F67EB3C3641DCC6F	FASTA	165	17,977
	10 20 30 40 50 60 MSATAEQNAR NPKGKGGFAR TVSQRKRKRL FLIGGALAVL AVAVGLMLTA FNQDIRFFRT 70 80 90 100 110 120 PADLTEQDMT SGARFRLGGL VEEGSVSRTG SELRFTVTDT IKTVKVVFEG IPPDLFREGQ 130 140 150 160 GVVAEGRFGS DGLFRADNVL AKHDENYVPK DLADSLKKKG VWEGK

References

[1]

"Whole-genome analyses of speciation events in pathogenic Brucellae." Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E. Infect. Immun. 73:8353-8361(2005) [PubMed: 16299333] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM040264 Genomic DNA. Translation: CAJ10588.1.
RefSeq	YP_414074.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2YMU7.
Genome annotation databases
GeneID	3788646.
GenomeReviews	Gene locus BAB1_0632 in contig AM040264_GR.
KEGG	bmf:BAB1_0632.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2YMU7.
OMA	HVEFAVH
Enzyme and pathway databases
BioCyc	BMEL359391:BAB1_0632-MON.
Family and domain databases
HAMAP	MF_01959. [Tree]
InterPro	IPR004329. CcmE. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF03100. CcmE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CCME_BRUA2

Accession

Primary (citable) accession number: Q2YMU7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	February 7, 2006
Last modified:	November 24, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)