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Protein

Modification methylase BabI

Gene

ccrM

Organism
Brucella abortus (strain 2308)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GANTC and causes specific methylation on A-2 on both strands. CcrM-mediated methylation has important cellular functions. Appears to contribute to the accurate cell-cycle control of DNA replication and cellular morphology. Essential for viability.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBABO359391:GKDV-523-MONOMER.
BMEL359391:GJOQ-523-MONOMER.

Protein family/group databases

REBASEi3263. M.BabI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase BabI (EC:2.1.1.72)
Short name:
M.BabI
Alternative name(s):
Adenine-specific methyltransferase BabI
DNA methyltransferase CcrM
M.CcrMI ortholog
Gene namesi
Name:ccrM
Synonyms:babI, babIM
Ordered Locus Names:BAB1_0516
OrganismiBrucella abortus (strain 2308)
Taxonomic identifieri359391 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000002719 Componenti: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 377377Modification methylase BabIPRO_0000087986Add
BLAST

Expressioni

Inductioni

By CtrA.Curated

Structurei

3D structure databases

ProteinModelPortaliQ2YMK2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Phylogenomic databases

eggNOGiCOG0863.
HOGENOMiHOG000125329.
KOiK13581.
OMAiFWHFEDG.
OrthoDBiEOG6FV84T.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR001091. RM_Methylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00508. S21N4MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2YMK2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLVRLAHEL PIEAPRTAWL DSIIKGDCVS ALERLPDHSV DVIFADPPYN
60 70 80 90 100
LQLGGDLHRP DQSMVSAVDD HWDQFESFQA YDAFTRAWLL ACRRVLKPNG
110 120 130 140 150
TIWVIGSYHN IFRVGTQLQD LGFWLLNDIV WRKTNPMPNF RGRRFQNAHE
160 170 180 190 200
TLIWASREQK GKGYTFNYEA MKAANDDVQM RSDWLFPICT GSERLKDENG
210 220 230 240 250
DKVHPTQKPE ALLARIMMAS SKPGDVILDP FFGSGTTGAV AKRLGRHFVG
260 270 280 290 300
IEREQPYIDA ATARINAVEP LGKAELTVMT GKRAEPRVAF TSVMEAGLLR
310 320 330 340 350
PGTVLCDERR RFAAIVRADG TLTANGEAGS IHRIGARVQG FDACNGWTFW
360 370
HFEENGVLKP IDALRKIIRE QMAAAGA
Length:377
Mass (Da):42,202
Last modified:December 20, 2005 - v1
Checksum:i657C88A25580B39D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF011895 Genomic DNA. Translation: AAB71351.1.
AM040264 Genomic DNA. Translation: CAJ10472.1.
RefSeqiWP_002969465.1. NZ_KN046823.1.
YP_413971.1. NC_007618.1.

Genome annotation databases

EnsemblBacteriaiCAJ10472; CAJ10472; BAB1_0516.
GeneIDi3788634.
KEGGibmf:BAB1_0516.
PATRICi17843906. VBIBruMel86222_0538.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF011895 Genomic DNA. Translation: AAB71351.1.
AM040264 Genomic DNA. Translation: CAJ10472.1.
RefSeqiWP_002969465.1. NZ_KN046823.1.
YP_413971.1. NC_007618.1.

3D structure databases

ProteinModelPortaliQ2YMK2.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3263. M.BabI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ10472; CAJ10472; BAB1_0516.
GeneIDi3788634.
KEGGibmf:BAB1_0516.
PATRICi17843906. VBIBruMel86222_0538.

Phylogenomic databases

eggNOGiCOG0863.
HOGENOMiHOG000125329.
KOiK13581.
OMAiFWHFEDG.
OrthoDBiEOG6FV84T.

Enzyme and pathway databases

BioCyciBABO359391:GKDV-523-MONOMER.
BMEL359391:GJOQ-523-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR001091. RM_Methylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00508. S21N4MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The CcrM DNA methyltransferase is widespread in the alpha subdivision of proteobacteria, and its essential functions are conserved in Rhizobium meliloti and Caulobacter crescentus."
    Wright R., Stephens C., Shapiro L.
    J. Bacteriol. 179:5869-5877(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 2308.
  3. "The Brucella abortus CcrM DNA methyltransferase is essential for viability, and its overexpression attenuates intracellular replication in murine macrophages."
    Robertson G.T., Reisenauer A., Wright R., Jensen R.B., Jensen A., Shapiro L., Roop R.M. II
    J. Bacteriol. 182:3482-3489(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: IMPORTANCE IN VIABILITY, TRANSCRIPTIONAL CONTROL BY CTRA.

Entry informationi

Entry nameiMTB1_BRUA2
AccessioniPrimary (citable) accession number: Q2YMK2
Secondary accession number(s): O30570, Q57EN0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: December 20, 2005
Last modified: June 24, 2015
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Brucella abortus strain 2308
    Brucella abortus (strain 2308): entries and gene names
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.