Reviewed,
UniProtKB/Swiss-Prot Q2YMK2 (MTB1_BRUA2)
Last modified
November 3, 2009.
Version 31.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Modification methylase BabI Short name=M.BabI EC=2.1.1.72 Alternative name(s): Adenine-specific methyltransferase BabI DNA methyltransferase ccrM M.CcrMI ortholog | ||||||
| Gene names |
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| Organism | Brucella abortus (strain 2308) [Complete proteome] [HAMAP] | ||||||
| Taxonomic identifier | 359391 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella |
Protein attributes
| Sequence length | 377 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | This methylase recognizes the double-stranded sequence GANTC and causes specific methylation on A-2 on both strands. CcrM-mediated methylation has important cellular functions. Appears to contribute to the accurate cell-cycle control of DNA replication and cellular morphology. Essential for viability. |
| Catalytic activity | S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine. |
| Induction | By ctrA Probable. |
| Sequence similarities | Belongs to the N(4)/N(6)-methyltransferase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | DNA replication |
| Ligand | S-adenosyl-L-methionine |
| Molecular function | Methyltransferase Transferase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | DNA methylation Inferred from electronic annotation. Source: InterPro DNA replicationInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activityInferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||
Molecule processing | |||||||
|---|---|---|---|---|---|---|---|
| Chain | 1 – 377 | 377 | Modification methylase BabI | PRO_0000087986 | |||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The CcrM DNA methyltransferase is widespread in the alpha subdivision of proteobacteria, and its essential functions are conserved in Rhizobium meliloti and Caulobacter crescentus." Wright R., Stephens C., Shapiro L. J. Bacteriol. 179:5869-5877(1997) [PubMed: 9294447] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "Whole-genome analyses of speciation events in pathogenic Brucellae." Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E. Infect. Immun. 73:8353-8361(2005) [PubMed: 16299333] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [3] | "The Brucella abortus CcrM DNA methyltransferase is essential for viability, and its overexpression attenuates intracellular replication in murine macrophages." Robertson G.T., Reisenauer A., Wright R., Jensen R.B., Jensen A., Shapiro L., Roop R.M. II J. Bacteriol. 182:3482-3489(2000) [PubMed: 10852881] [Abstract] Cited for: IMPORTANCE IN VIABILITY, TRANSCRIPTIONAL CONTROL BY CTRA. |
Cross-references
Sequence databases | |
|---|---|
| AF011895 Genomic DNA. Translation: AAB71351.1. AM040264 Genomic DNA. Translation: CAJ10472.1. | |
| RefSeq | YP_413971.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1G60 based on UniProtKB P23192. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q2YMK2. |
Protein family/group databases | |
| REBASE | 3263. M.BabI. |
Genome annotation databases | |
| GeneID | 3788634. |
| GenomeReviews | Gene locus BAB1_0516 in contig AM040264_GR. |
| KEGG | bmf:BAB1_0516. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | Q2YMK2. |
| OMA | WILNDII. |
Enzyme and pathway databases | |
| BioCyc | BMEL359391:BAB1_0516-MON. |
Family and domain databases | |
| InterPro | IPR002941. DNA_methylase_N4/N6. IPR002052. DNA_methylase_N6_adenine_CS. IPR001091. MeTrfase_CN4. [Graphical view] |
| Pfam | PF01555. N6_N4_Mtase. 1 hit. [Graphical view] |
| PRINTS | PR00508. S21N4MTFRASE. |
| PROSITE | PS00092. N6_MTASE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | MTB1_BRUA2 | ||||||||
| Accession | Primary (citable) accession number: Q2YMK2 Secondary accession number(s): O30570, Q57EN0 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Brucella abortus strain 2308 Brucella abortus (strain 2308): entries and gene names |
| Restriction enzymes and methylases Classification of restriction enzymes and methylases and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


