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Q2YMK2

- MTB1_BRUA2

UniProt

Q2YMK2 - MTB1_BRUA2

Protein

Modification methylase BabI

Gene

ccrM

Organism
Brucella abortus (strain 2308)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (20 Dec 2005)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence GANTC and causes specific methylation on A-2 on both strands. CcrM-mediated methylation has important cellular functions. Appears to contribute to the accurate cell-cycle control of DNA replication and cellular morphology. Essential for viability.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. N-methyltransferase activity Source: InterPro
    3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciBABO359391:GKDV-523-MONOMER.
    BMEL359391:GJOQ-523-MONOMER.

    Protein family/group databases

    REBASEi3263. M.BabI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase BabI (EC:2.1.1.72)
    Short name:
    M.BabI
    Alternative name(s):
    Adenine-specific methyltransferase BabI
    DNA methyltransferase CcrM
    M.CcrMI ortholog
    Gene namesi
    Name:ccrM
    Synonyms:babI, babIM
    Ordered Locus Names:BAB1_0516
    OrganismiBrucella abortus (strain 2308)
    Taxonomic identifieri359391 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
    ProteomesiUP000002719: Chromosome I

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 377377Modification methylase BabIPRO_0000087986Add
    BLAST

    Expressioni

    Inductioni

    By CtrA.Curated

    Interactioni

    Protein-protein interaction databases

    STRINGi359391.BAB1_0516.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2YMK2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Phylogenomic databases

    eggNOGiCOG0863.
    HOGENOMiHOG000125329.
    KOiK13581.
    OMAiFWHFEDG.
    OrthoDBiEOG6FV84T.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR002941. DNA_methylase_N4/N6.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR001091. RM_Methylase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF01555. N6_N4_Mtase. 1 hit.
    [Graphical view]
    PRINTSiPR00508. S21N4MTFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS00092. N6_MTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q2YMK2-1 [UniParc]FASTAAdd to Basket

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    MSLVRLAHEL PIEAPRTAWL DSIIKGDCVS ALERLPDHSV DVIFADPPYN    50
    LQLGGDLHRP DQSMVSAVDD HWDQFESFQA YDAFTRAWLL ACRRVLKPNG 100
    TIWVIGSYHN IFRVGTQLQD LGFWLLNDIV WRKTNPMPNF RGRRFQNAHE 150
    TLIWASREQK GKGYTFNYEA MKAANDDVQM RSDWLFPICT GSERLKDENG 200
    DKVHPTQKPE ALLARIMMAS SKPGDVILDP FFGSGTTGAV AKRLGRHFVG 250
    IEREQPYIDA ATARINAVEP LGKAELTVMT GKRAEPRVAF TSVMEAGLLR 300
    PGTVLCDERR RFAAIVRADG TLTANGEAGS IHRIGARVQG FDACNGWTFW 350
    HFEENGVLKP IDALRKIIRE QMAAAGA 377
    Length:377
    Mass (Da):42,202
    Last modified:December 20, 2005 - v1
    Checksum:i657C88A25580B39D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF011895 Genomic DNA. Translation: AAB71351.1.
    AM040264 Genomic DNA. Translation: CAJ10472.1.
    RefSeqiWP_002969465.1. NC_007618.1.
    YP_413971.1. NC_007618.1.

    Genome annotation databases

    EnsemblBacteriaiCAJ10472; CAJ10472; BAB1_0516.
    GeneIDi3788634.
    KEGGibmf:BAB1_0516.
    PATRICi17843906. VBIBruMel86222_0538.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF011895 Genomic DNA. Translation: AAB71351.1 .
    AM040264 Genomic DNA. Translation: CAJ10472.1 .
    RefSeqi WP_002969465.1. NC_007618.1.
    YP_413971.1. NC_007618.1.

    3D structure databases

    ProteinModelPortali Q2YMK2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 359391.BAB1_0516.

    Protein family/group databases

    REBASEi 3263. M.BabI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAJ10472 ; CAJ10472 ; BAB1_0516 .
    GeneIDi 3788634.
    KEGGi bmf:BAB1_0516.
    PATRICi 17843906. VBIBruMel86222_0538.

    Phylogenomic databases

    eggNOGi COG0863.
    HOGENOMi HOG000125329.
    KOi K13581.
    OMAi FWHFEDG.
    OrthoDBi EOG6FV84T.

    Enzyme and pathway databases

    BioCyci BABO359391:GKDV-523-MONOMER.
    BMEL359391:GJOQ-523-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR002941. DNA_methylase_N4/N6.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR001091. RM_Methylase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF01555. N6_N4_Mtase. 1 hit.
    [Graphical view ]
    PRINTSi PR00508. S21N4MTFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS00092. N6_MTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The CcrM DNA methyltransferase is widespread in the alpha subdivision of proteobacteria, and its essential functions are conserved in Rhizobium meliloti and Caulobacter crescentus."
      Wright R., Stephens C., Shapiro L.
      J. Bacteriol. 179:5869-5877(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 2308.
    3. "The Brucella abortus CcrM DNA methyltransferase is essential for viability, and its overexpression attenuates intracellular replication in murine macrophages."
      Robertson G.T., Reisenauer A., Wright R., Jensen R.B., Jensen A., Shapiro L., Roop R.M. II
      J. Bacteriol. 182:3482-3489(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: IMPORTANCE IN VIABILITY, TRANSCRIPTIONAL CONTROL BY CTRA.

    Entry informationi

    Entry nameiMTB1_BRUA2
    AccessioniPrimary (citable) accession number: Q2YMK2
    Secondary accession number(s): O30570, Q57EN0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 7, 2006
    Last sequence update: December 20, 2005
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Brucella abortus strain 2308
      Brucella abortus (strain 2308): entries and gene names
    2. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3