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Q2YMK2 (MTB1_BRUA2) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase BabI

Short name=M.BabI
EC=2.1.1.72
Alternative name(s):
Adenine-specific methyltransferase BabI
DNA methyltransferase CcrM
M.CcrMI ortholog
Gene names
Name:ccrM
Synonyms:babI, babIM
Ordered Locus Names:BAB1_0516
OrganismBrucella abortus (strain 2308) [Complete proteome] [HAMAP]
Taxonomic identifier359391 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length377 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GANTC and causes specific methylation on A-2 on both strands. CcrM-mediated methylation has important cellular functions. Appears to contribute to the accurate cell-cycle control of DNA replication and cellular morphology. Essential for viability.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Induction

By CtrA Probable.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 377377Modification methylase BabI
PRO_0000087986

Sequences

Sequence LengthMass (Da)Tools
Q2YMK2 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: 657C88A25580B39D

FASTA37742,202
        10         20         30         40         50         60 
MSLVRLAHEL PIEAPRTAWL DSIIKGDCVS ALERLPDHSV DVIFADPPYN LQLGGDLHRP 

        70         80         90        100        110        120 
DQSMVSAVDD HWDQFESFQA YDAFTRAWLL ACRRVLKPNG TIWVIGSYHN IFRVGTQLQD 

       130        140        150        160        170        180 
LGFWLLNDIV WRKTNPMPNF RGRRFQNAHE TLIWASREQK GKGYTFNYEA MKAANDDVQM 

       190        200        210        220        230        240 
RSDWLFPICT GSERLKDENG DKVHPTQKPE ALLARIMMAS SKPGDVILDP FFGSGTTGAV 

       250        260        270        280        290        300 
AKRLGRHFVG IEREQPYIDA ATARINAVEP LGKAELTVMT GKRAEPRVAF TSVMEAGLLR 

       310        320        330        340        350        360 
PGTVLCDERR RFAAIVRADG TLTANGEAGS IHRIGARVQG FDACNGWTFW HFEENGVLKP 

       370 
IDALRKIIRE QMAAAGA 

« Hide

References

« Hide 'large scale' references
[1]"The CcrM DNA methyltransferase is widespread in the alpha subdivision of proteobacteria, and its essential functions are conserved in Rhizobium meliloti and Caulobacter crescentus."
Wright R., Stephens C., Shapiro L.
J. Bacteriol. 179:5869-5877(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Whole-genome analyses of speciation events in pathogenic Brucellae."
Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E.
Infect. Immun. 73:8353-8361(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 2308.
[3]"The Brucella abortus CcrM DNA methyltransferase is essential for viability, and its overexpression attenuates intracellular replication in murine macrophages."
Robertson G.T., Reisenauer A., Wright R., Jensen R.B., Jensen A., Shapiro L., Roop R.M. II
J. Bacteriol. 182:3482-3489(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: IMPORTANCE IN VIABILITY, TRANSCRIPTIONAL CONTROL BY CTRA.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF011895 Genomic DNA. Translation: AAB71351.1.
AM040264 Genomic DNA. Translation: CAJ10472.1.
RefSeqYP_413971.1. NC_007618.1.

3D structure databases

ProteinModelPortalQ2YMK2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING359391.BAB1_0516.

Protein family/group databases

REBASE3263. M.BabI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAJ10472; CAJ10472; BAB1_0516.
GeneID3788634.
KEGGbmf:BAB1_0516.
PATRIC17843906. VBIBruMel86222_0538.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0863.
HOGENOMHOG000125329.
KOK13581.
OMAFWHFEDG.
OrthoDBEOG6FV84T.
ProtClustDBCLSK2518600.

Enzyme and pathway databases

BioCycBABO359391:GKDV-523-MONOMER.
BMEL359391:GJOQ-523-MONOMER.

Family and domain databases

InterProIPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR001091. RM_Methylase.
[Graphical view]
PfamPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSPR00508. S21N4MTFRASE.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTB1_BRUA2
AccessionPrimary (citable) accession number: Q2YMK2
Secondary accession number(s): O30570, Q57EN0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: December 20, 2005
Last modified: April 16, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries

Brucella abortus strain 2308

Brucella abortus (strain 2308): entries and gene names