Q2YMK2 (MTB1_BRUA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase BabI
Short name=M.BabI
EC=2.1.1.72

Alternative name(s):
Adenine-specific methyltransferase BabI
DNA methyltransferase CcrM
M.CcrMI ortholog

Gene names

Name:	ccrM
Synonyms:	babI, babIM
Ordered Locus Names:	BAB1_0516

Organism

Brucella abortus (strain 2308) [Complete proteome] [HAMAP]

Taxonomic identifier

359391 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella abortus

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GANTC and causes specific methylation on A-2 on both strands. CcrM-mediated methylation has important cellular functions. Appears to contribute to the accurate cell-cycle control of DNA replication and cellular morphology. Essential for viability.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Induction	By CtrA Probable.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA methylation on adenine Inferred from electronic annotation. Source: GOC DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 377	377	Modification methylase BabI		PRO_0000087986

Sequences

	Sequence		Length	Mass (Da)
	Q2YMK2 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 657C88A25580B39D	FASTA	377	42,202
	10 20 30 40 50 60 MSLVRLAHEL PIEAPRTAWL DSIIKGDCVS ALERLPDHSV DVIFADPPYN LQLGGDLHRP 70 80 90 100 110 120 DQSMVSAVDD HWDQFESFQA YDAFTRAWLL ACRRVLKPNG TIWVIGSYHN IFRVGTQLQD 130 140 150 160 170 180 LGFWLLNDIV WRKTNPMPNF RGRRFQNAHE TLIWASREQK GKGYTFNYEA MKAANDDVQM 190 200 210 220 230 240 RSDWLFPICT GSERLKDENG DKVHPTQKPE ALLARIMMAS SKPGDVILDP FFGSGTTGAV 250 260 270 280 290 300 AKRLGRHFVG IEREQPYIDA ATARINAVEP LGKAELTVMT GKRAEPRVAF TSVMEAGLLR 310 320 330 340 350 360 PGTVLCDERR RFAAIVRADG TLTANGEAGS IHRIGARVQG FDACNGWTFW HFEENGVLKP 370 IDALRKIIRE QMAAAGA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The CcrM DNA methyltransferase is widespread in the alpha subdivision of proteobacteria, and its essential functions are conserved in Rhizobium meliloti and Caulobacter crescentus." Wright R., Stephens C., Shapiro L. J. Bacteriol. 179:5869-5877(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Whole-genome analyses of speciation events in pathogenic Brucellae." Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E. Infect. Immun. 73:8353-8361(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 2308.
[3]	"The Brucella abortus CcrM DNA methyltransferase is essential for viability, and its overexpression attenuates intracellular replication in murine macrophages." Robertson G.T., Reisenauer A., Wright R., Jensen R.B., Jensen A., Shapiro L., Roop R.M. II J. Bacteriol. 182:3482-3489(2000) [PubMed] [Europe PMC] [Abstract] Cited for: IMPORTANCE IN VIABILITY, TRANSCRIPTIONAL CONTROL BY CTRA.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF011895 Genomic DNA. Translation: AAB71351.1. AM040264 Genomic DNA. Translation: CAJ10472.1.
RefSeq	YP_413971.1. NC_007618.1.
3D structure databases
ProteinModelPortal	Q2YMK2.
ModBase	Search...
Protein-protein interaction databases
STRING	359391.BAB1_0516.
Protein family/group databases
REBASE	3263. M.BabI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAJ10472; CAJ10472; BAB1_0516.
GeneID	3788634.
KEGG	bmf:BAB1_0516.
PATRIC	17843906. VBIBruMel86222_0538.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0863.
HOGENOM	HOG000125329.
KO	K13581.
OMA	KVHSTQK.
ProtClustDB	CLSK2518600.
Enzyme and pathway databases
BioCyc	BMEL359391:GJOQ-523-MONOMER.
Family and domain databases
InterPro	IPR002941. DNA_methylase_N4/N6. IPR002052. DNA_methylase_N6_adenine_CS. IPR001091. RM_Methylase. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PRINTS	PR00508. S21N4MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTB1_BRUA2

Accession

Primary (citable) accession number: Q2YMK2
Secondary accession number(s): O30570, Q57EN0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	December 20, 2005
Last modified:	May 1, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program