Q2YMB3 (NHAA_BRUA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Na(+)/H(+) antiporter NhaA

Alternative name(s):
Sodium/proton antiporter NhaA

Gene names

Name:	nhaA
Ordered Locus Names:	BAB1_0425

Organism

Brucella abortus (strain 2308) [Complete proteome] [HAMAP]

Taxonomic identifier

359391 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella abortus

Protein attributes

Sequence length	736 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Na+/H+ antiporter that extrudes sodium in exchange for external protons By similarity. HAMAP-Rule MF_01844
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_01844.
Sequence similarities	In the N-terminal section; belongs to the NhaA Na(+)/H(+) (TC 2.A.33) antiporter family. [View classification] In the C-terminal section; belongs to the peptidase S49 family.
Sequence caution	The sequence CAJ10381.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Antiport Ion transport Sodium transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Sodium
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: InterPro regulation of pH Inferred from electronic annotation. Source: InterPro sodium ion transmembrane transport Inferred from electronic annotation. Source: GOC
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	antiporter activity Inferred from electronic annotation. Source: HAMAP peptidase activity Inferred from electronic annotation. Source: InterPro sodium ion transmembrane transporter activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 736	736	Na(+)/H(+) antiporter NhaA HAMAP-Rule MF_01844		PRO_0000334475
Regions
	Transmembrane	23 – 43	21	Helical; Potential
	Transmembrane	58 – 78	21	Helical; Potential
	Transmembrane	96 – 116	21	Helical; Potential
	Transmembrane	126 – 146	21	Helical; Potential
	Transmembrane	155 – 175	21	Helical; Potential
	Transmembrane	178 – 198	21	Helical; Potential
	Transmembrane	224 – 244	21	Helical; Potential
	Transmembrane	265 – 285	21	Helical; Potential
	Transmembrane	298 – 318	21	Helical; Potential
	Transmembrane	334 – 354	21	Helical; Potential
	Transmembrane	367 – 387	21	Helical; Potential
	Region	1 – 387	387	Na(+)/H(+) antiporter NhaA HAMAP-Rule MF_01844
	Region	388 – 736	349	Peptidase S49 HAMAP-Rule MF_01844

Sequences

	Sequence		Length	Mass (Da)
	Q2YMB3 [UniParc]. Last modified May 20, 2008. Version 2. Checksum: 7C5E5742B5EC5654	FASTA	736	78,587
	10 20 30 40 50 60 MNHSPQSARP VSIMRRFLDS EAAGGITLMA AAALALIVAN SPFAQTYFDA LHLYIGPLSL 70 80 90 100 110 120 AHWINDALMA IFFLLVGLEI KREMLDGQLA SWPNRMLPGI AAAGGVILPA IIFAVLNHDN 130 140 150 160 170 180 PAKLRGWAVP SATDIAFALG VLSLLGSRAP SSLKVFLATL AILDDLAAVV IIAIFYTAEI 190 200 210 220 230 240 SMPYLGAAFI TAAVLFVMNR MGVVKLLPYL ISAVILWFFV FNSGVHATVA GVVAALMIPL 250 260 270 280 290 300 KPAPGRPDDM TSPLHKLEHA LAKPVAFIVV PIFGFANAGI SFKGLEASVL GDTLTLGILL 310 320 330 340 350 360 GLFLGKQFGV FGAAWLAIKT GLAEKPMGAS WVQLYGVAIL CGIGFTMSIF IGLLSFPSDL 370 380 390 400 410 420 MQTETKIGVL SGSALSAICG YLLLRAARPD QSAANPLWKA DESPEAKNFG RFLCVFHFAS 430 440 450 460 470 480 YIASTYCTER LQAASSLLRR SSLEFALPGL LKRLIPRRFR AVETEIPVVR LHGAIMTGGT 490 500 510 520 530 540 SLRPTLSLAS TAGILEKAFA DKHAPAVAIS INSPGGAPVQ SRLIYRRIRD LAVEHQKKVF 550 560 570 580 590 600 VFVEDVAASG GYMIALAGDE IIADPSSIVG SIGVVSASFG FPELLKKIGV ERRVYTAGSN 610 620 630 640 650 660 KVTLDPFQPE KAEDIERLKA LQLEIHATFI DMVKERRAGK LGDNPDLFSG LFWTGTTAAS 670 680 690 700 710 720 LGLIDGLGDM RSFLRKTYGD KVKLKLIQPQ RGLLGRKLPG IGMDSGSVEP AQIAAHLGDG 730 LLCVAEEKAI WARYGL

References

[1]

"Whole-genome analyses of speciation events in pathogenic Brucellae." Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E. Infect. Immun. 73:8353-8361(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 2308.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM040264 Genomic DNA. Translation: CAJ10381.1. Different initiation.
RefSeq	YP_413885.1. NC_007618.1.
3D structure databases
ProteinModelPortal	Q2YMB3.
ModBase	Search...
Protein-protein interaction databases
STRING	359391.BAB1_0425.
Proteomic databases
PRIDE	Q2YMB3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAJ10381; CAJ10381; BAB1_0425.
GeneID	3787184.
KEGG	bmf:BAB1_0425.
PATRIC	17843722. VBIBruMel86222_0446.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3004.
HOGENOM	HOG000060687.
KO	K03313.
ProtClustDB	CLSK383673.
Enzyme and pathway databases
BioCyc	BMEL359391:GJOQ-432-MONOMER.
Family and domain databases
Gene3D	1.20.1530.10. 1 hit.
HAMAP	MF_01844. NhaA. Fused.
InterPro	IPR023171. Na/H_antiporter_dom. IPR004670. NhaA. IPR002142. Peptidase_S49. [Graphical view]
Pfam	PF06965. Na_H_antiport_1. 1 hit. PF01343. Peptidase_S49. 1 hit. [Graphical view]
TIGRFAMs	TIGR00773. NhaA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NHAA_BRUA2

Accession

Primary (citable) accession number: Q2YMB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 20, 2008
Last modified:	May 1, 2013
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program