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Q2YM22 (CARA_BRUA2) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Carbamoyl-phosphate synthase small chain

EC=6.3.5.5
Alternative name(s):
Carbamoyl-phosphate synthetase glutamine chain
Gene names
Name:carA
Ordered Locus Names:BAB1_1502
OrganismBrucella abortus (strain 2308) [Complete proteome] [HAMAP]
Taxonomic identifier359391 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length407 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP-Rule MF_01209

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. HAMAP-Rule MF_01209

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP-Rule MF_01209

Subunit structure

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.

Sequence similarities

Belongs to the CarA family.

Contains 1 glutamine amidotransferase type-1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 407407Carbamoyl-phosphate synthase small chain HAMAP-Rule MF_01209
PRO_1000138853

Regions

Domain209 – 397189Glutamine amidotransferase type-1
Region1 – 205205CPSase HAMAP-Rule MF_01209

Sites

Active site2861Nucleophile By similarity
Active site3701 By similarity
Active site3721 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2YM22 [UniParc].

Last modified February 7, 2006. Version 1.
Checksum: D6C01AFF0592F7E2

FASTA40743,536
        10         20         30         40         50         60 
MTETTPKTAP WTVQKRTAVL VLADGTVIEG KGLGATGAVE AEVVFNTALT GYEEILTDPS 

        70         80         90        100        110        120 
YAGQIVTFTF PHIGNVGANA EDIEDLTPAN RHGAVGAIFK ADITAPSNFR AAKDLDSWLK 

       130        140        150        160        170        180 
HRGIIALAGI DTRALTALIR ERGAQNAVIA HDPNGNFDLD ALKARAANWC GLENLDLAKD 

       190        200        210        220        230        240 
VTIGQSLVWK ELPWTLQDGY GEQDAPQYHV VALDFGVKRN ILRLLTGLGA KVTVLPATAT 

       250        260        270        280        290        300 
AEDVLAHNPD GVFLSNGPGD PAATGEYAVP TIGKLVETGI PLFGICLGHQ MLALALGGRT 

       310        320        330        340        350        360 
EKMHQGHHGA NHPVKDYTTG KVEIVSMNHG FAVDSDSLPE NVEETHVSLF DGTNCGLRVV 

       370        380        390        400 
GKPVFSVQHH PEASPGPQDS HYLFRRFINL IRERKGQAPL PEREQAA 

« Hide

References

[1]"Whole-genome analyses of speciation events in pathogenic Brucellae."
Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E.
Infect. Immun. 73:8353-8361(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 2308.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM040264 Genomic DNA. Translation: CAJ11458.1.
RefSeqYP_414870.1. NC_007618.1.

3D structure databases

ProteinModelPortalQ2YM22.
SMRQ2YM22. Positions 16-396.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING359391.BAB1_1502.

Proteomic databases

PRIDEQ2YM22.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAJ11458; CAJ11458; BAB1_1502.
GeneID3788048.
KEGGbmf:BAB1_1502.
PATRIC17845997. VBIBruMel86222_1560.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0505.
HOGENOMHOG000038087.
KOK01956.
OMAKTIKMKF.
OrthoDBEOG61ZTH6.

Enzyme and pathway databases

BioCycBABO359391:GKDV-1535-MONOMER.
BMEL359391:GJOQ-1535-MONOMER.
UniPathwayUPA00068; UER00171.
UPA00070; UER00115.

Family and domain databases

Gene3D3.40.50.880. 1 hit.
3.50.30.20. 1 hit.
HAMAPMF_01209. CPSase_S_chain.
InterProIPR006274. CarbamoylP_synth_ssu.
IPR002474. CarbamoylP_synth_ssu_N.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
[Graphical view]
PfamPF00988. CPSase_sm_chain. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SMARTSM01097. CPSase_sm_chain. 1 hit.
[Graphical view]
SUPFAMSSF52021. SSF52021. 1 hit.
SSF52317. SSF52317. 1 hit.
TIGRFAMsTIGR01368. CPSaseIIsmall. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ2YM22.

Entry information

Entry nameCARA_BRUA2
AccessionPrimary (citable) accession number: Q2YM22
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 7, 2006
Last modified: June 11, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Brucella abortus strain 2308

Brucella abortus (strain 2308): entries and gene names