Q2YLM5 (HEM3_BRUA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Porphobilinogen deaminase
Short name=PBG
EC=2.5.1.61

Alternative name(s):
Hydroxymethylbilane synthase
Short name=HMBS
Pre-uroporphyrinogen synthase

Gene names

Name:	hemC
Ordered Locus Names:	BAB1_1887

Organism

Brucella abortus (strain 2308) [Complete proteome] [HAMAP]

Taxonomic identifier

359391 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella abortus

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps By similarity. HAMAP-Rule MF_00260
Catalytic activity	4 porphobilinogen + H2O = hydroxymethylbilane + 4 NH3. HAMAP-Rule MF_00260
Cofactor	Binds 1 dipyrromethane group covalently By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4. HAMAP-Rule MF_00260
Subunit structure	Monomer By similarity.
Miscellaneous	The porphobilinogen subunits are added to the dipyrromethane group By similarity. HAMAP-Rule MF_00260
Sequence similarities	Belongs to the HMBS family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: HAMAP peptidyl-pyrromethane cofactor linkage Inferred from electronic annotation. Source: InterPro protoporphyrinogen IX biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	hydroxymethylbilane synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	Porphobilinogen deaminase HAMAP-Rule MF_00260		PRO_0000304217
Amino acid modifications
	Modified residue	249	1	S-(dipyrrolylmethanemethyl)cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2YLM5 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: D4F34A01D72322B6	FASTA	314	33,838
	10 20 30 40 50 60 MQTASFKNGT LKIGTRGSKL ALAQAYLTRR LLQEAHGLPE DAIEILPMST AGDRIQDRPL 70 80 90 100 110 120 SEVGGKGLFT EEIEQALKDG RIDIAVHSTK DMPTALPEGL HLSVFLERED PRDAFIGRSA 130 140 150 160 170 180 RRFMDLPQGA TVGSSSLRRQ ALIRRLRPDI EVVMYRGNVD TRLRKLDAGE VDGTFLACAG 190 200 210 220 230 240 LRRLGLADVI TDVLDPSVFP PAPGQGAIGI ESRIGDERID VLLAPLAHRE TQIALACERA 250 260 270 280 290 300 FLGALDGSCR TPIAGLATVE GDRLSFRGMI LTPDGRQAHE VTAEGVVSDA AALGTDAANR 310 VRAMAGPHFF DGWQ

References

[1]

"Whole-genome analyses of speciation events in pathogenic Brucellae." Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E. Infect. Immun. 73:8353-8361(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 2308.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM040264 Genomic DNA. Translation: CAJ11843.1.
RefSeq	YP_415229.1. NC_007618.1.
3D structure databases
ProteinModelPortal	Q2YLM5.
ModBase	Search...
Protein-protein interaction databases
STRING	359391.BAB1_1887.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAJ11843; CAJ11843; BAB1_1887.
GeneID	3788370.
KEGG	bmf:BAB1_1887.
PATRIC	17846846. VBIBruMel86222_1964.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0181.
HOGENOM	HOG000228588.
KO	K01749.
OMA	CLAERAF.
ProtClustDB	PRK00072.
Enzyme and pathway databases
BioCyc	BMEL359391:GJOQ-1938-MONOMER.
UniPathway	UPA00251; UER00319.
Family and domain databases
Gene3D	3.30.160.40. 1 hit.
HAMAP	MF_00260. Porphobil_deam.
InterPro	IPR000860. 4pyrrol_synth_OHMeBilane_synth. IPR022419. Porphobilin_deaminase_cofac_BS. IPR022417. Porphobilin_deaminase_N. IPR022418. Porphobilinogen_deaminase_C. [Graphical view]
PANTHER	PTHR11557. PTHR11557. 1 hit.
Pfam	PF01379. Porphobil_deam. 1 hit. PF03900. Porphobil_deamC. 1 hit. [Graphical view]
PIRSF	PIRSF001438. 4pyrrol_synth_OHMeBilane_synth. 1 hit.
PRINTS	PR00151. PORPHBDMNASE.
SUPFAM	SSF54782. Porphobil_deam. 1 hit.
TIGRFAMs	TIGR00212. hemC. 1 hit.
PROSITE	PS00533. PORPHOBILINOGEN_DEAM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM3_BRUA2

Accession

Primary (citable) accession number: Q2YLM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	February 7, 2006
Last modified:	May 1, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program