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Q2YLM5 (HEM3_BRUA2) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Porphobilinogen deaminase

Short name=PBG
EC=2.5.1.61
Alternative name(s):
Hydroxymethylbilane synthase
Short name=HMBS
Pre-uroporphyrinogen synthase
Gene names
Name:hemC
Ordered Locus Names:BAB1_1887
OrganismBrucella abortus (strain 2308) [Complete proteome] [HAMAP]
Taxonomic identifier359391 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length314 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps By similarity. HAMAP-Rule MF_00260

Catalytic activity

4 porphobilinogen + H2O = hydroxymethylbilane + 4 NH3. HAMAP-Rule MF_00260

Cofactor

Binds 1 dipyrromethane group covalently By similarity. HAMAP-Rule MF_00260

Pathway

Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4. HAMAP-Rule MF_00260

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00260

Miscellaneous

The porphobilinogen subunits are added to the dipyrromethane group By similarity.

Sequence similarities

Belongs to the HMBS family.

Ontologies

Keywords
   Biological processPorphyrin biosynthesis
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processpeptidyl-pyrromethane cofactor linkage

Inferred from electronic annotation. Source: InterPro

protoporphyrinogen IX biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionhydroxymethylbilane synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 314314Porphobilinogen deaminase HAMAP-Rule MF_00260
PRO_0000304217

Amino acid modifications

Modified residue2491S-(dipyrrolylmethanemethyl)cysteine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2YLM5 [UniParc].

Last modified February 7, 2006. Version 1.
Checksum: D4F34A01D72322B6

FASTA31433,838
        10         20         30         40         50         60 
MQTASFKNGT LKIGTRGSKL ALAQAYLTRR LLQEAHGLPE DAIEILPMST AGDRIQDRPL 

        70         80         90        100        110        120 
SEVGGKGLFT EEIEQALKDG RIDIAVHSTK DMPTALPEGL HLSVFLERED PRDAFIGRSA 

       130        140        150        160        170        180 
RRFMDLPQGA TVGSSSLRRQ ALIRRLRPDI EVVMYRGNVD TRLRKLDAGE VDGTFLACAG 

       190        200        210        220        230        240 
LRRLGLADVI TDVLDPSVFP PAPGQGAIGI ESRIGDERID VLLAPLAHRE TQIALACERA 

       250        260        270        280        290        300 
FLGALDGSCR TPIAGLATVE GDRLSFRGMI LTPDGRQAHE VTAEGVVSDA AALGTDAANR 

       310 
VRAMAGPHFF DGWQ 

« Hide

References

[1]"Whole-genome analyses of speciation events in pathogenic Brucellae."
Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E.
Infect. Immun. 73:8353-8361(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 2308.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM040264 Genomic DNA. Translation: CAJ11843.1.
RefSeqYP_415229.1. NC_007618.1.

3D structure databases

ProteinModelPortalQ2YLM5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING359391.BAB1_1887.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAJ11843; CAJ11843; BAB1_1887.
GeneID3788370.
KEGGbmf:BAB1_1887.
PATRIC17846846. VBIBruMel86222_1964.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0181.
HOGENOMHOG000228588.
KOK01749.
OMAGAICIES.
OrthoDBEOG6HB9Z6.

Enzyme and pathway databases

BioCycBABO359391:GKDV-1938-MONOMER.
BMEL359391:GJOQ-1938-MONOMER.
UniPathwayUPA00251; UER00319.

Family and domain databases

Gene3D3.30.160.40. 1 hit.
HAMAPMF_00260. Porphobil_deam.
InterProIPR000860. 4pyrrol_synth_OHMeBilane_synth.
IPR022419. Porphobilin_deaminase_cofac_BS.
IPR022417. Porphobilin_deaminase_N.
IPR022418. Porphobilinogen_deaminase_C.
[Graphical view]
PANTHERPTHR11557. PTHR11557. 1 hit.
PfamPF01379. Porphobil_deam. 1 hit.
PF03900. Porphobil_deamC. 1 hit.
[Graphical view]
PIRSFPIRSF001438. 4pyrrol_synth_OHMeBilane_synth. 1 hit.
PRINTSPR00151. PORPHBDMNASE.
SUPFAMSSF54782. SSF54782. 1 hit.
TIGRFAMsTIGR00212. hemC. 1 hit.
PROSITEPS00533. PORPHOBILINOGEN_DEAM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHEM3_BRUA2
AccessionPrimary (citable) accession number: Q2YLM5
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: February 7, 2006
Last modified: May 14, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Brucella abortus strain 2308

Brucella abortus (strain 2308): entries and gene names