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Reviewed, UniProtKB/Swiss-Prot Q2YL58 (LEU3_BRUA2)

Last modified February 9, 2010. Version 30. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    3-isopropylmalate dehydrogenase
    EC=1.1.1.85
Alternative name(s):
    Beta-IPM dehydrogenase
      Short name=IMDH
    3-IPM-DH
Gene names
Name: leuB
Ordered Locus Names: BAB2_0346
OrganismBrucella abortus (strain 2308) [Complete proteome] [HAMAP]
Taxonomic identifier359391 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

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Protein attributes

Sequence length370 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate By similarity. HAMAP MF_01033

Catalytic activity

(2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH. HAMAP MF_01033

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity. HAMAP MF_01033

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4. HAMAP MF_01033

Subunit structure

Homodimer By similarity. HAMAP MF_01033

Subcellular location

Cytoplasm By similarity HAMAP MF_01033.

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3703703-isopropylmalate dehydrogenase HAMAP MF_01033
PRO_0000250106

Regions

Nucleotide binding77 – 9014NAD By similarity
Nucleotide binding290 – 30213NAD By similarity

Sites

Metal binding2261Magnesium or manganese By similarity
Metal binding2501Magnesium or manganese By similarity
Metal binding2541Magnesium or manganese By similarity
Binding site971Substrate By similarity
Binding site1071Substrate By similarity
Binding site1351Substrate By similarity
Binding site2261Substrate By similarity
Site1421Important for catalysis By similarity
Site1931Important for catalysis By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q2YL58-1 [UniParc].

Last modified February 7, 2006. Version 1.
Checksum: 5A389C4E26E82DFF

FASTA37039,730
        10         20         30         40         50         60 
MASRKLLLLP GDGIGPEAMA EVRKVIAFLN SDLNLGFETE EGLVGGCAYD AHGQAISDAD 

        70         80         90        100        110        120 
MEKALAADSV LFGAVGGPKW DSVPYEVRPE AGLLRLRKDM QLYANLRPAI CYPALAHSSS 

       130        140        150        160        170        180 
LKPEVIEGLD ILILRELTGG VYFGEPKEII DLGNGQKRGI DTQVYDTYEI ERIADVAFEL 

       190        200        210        220        230        240 
ARTRRNKVTS MEKRNVMKSG VLWNQGVTAR HKEKHADVQL EHMLADAGGM QLVRWPKQFD 

       250        260        270        280        290        300 
VILTDNLFGD LLSDVAAMLT GSLGMLPSAS LGAADSKTGK RKALYEPVHG SAPDIAGKGI 

       310        320        330        340        350        360 
ANPIAMIASL AMCLRYSFGL VAEADRLEAA IAGVLDDGIR TADIWSEGNT KVGTTEMGDA 

       370 
ILAKFKALSA

« Hide

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References

[1]"Whole-genome analyses of speciation events in pathogenic Brucellae."
Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E.
Infect. Immun. 73:8353-8361(2005) [PubMed: 16299333] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AM040265 Genomic DNA. Translation: CAJ12512.1.
RefSeqYP_418553.1.

3D structure databases

HSSPHSSP built from PDB template 1VLC based on UniProtKB Q9WZ26.
SMRQ2YL58. Positions 4-365.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ2YL58.

Genome annotation databases

GeneID3827559.
GenomeReviewsGene locus BAB2_0346 in contig AM040265_GR.
KEGGbmf:BAB2_0346.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0473.
HOGENOMHBG518924.
OMAMSYQIAV.

Enzyme and pathway databases

BioCycBMEL359391:BAB2_0346-MONOMER.

Family and domain databases

HAMAPMF_01033. LeuB_type1.
[Tree]
InterProIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004429. Isopropylmalate_DH.
[Graphical view]
Gene3DG3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHERPTHR11835. IDH_IMDH_dimeric. 1 hit.
PTHR11835:SF13. IPMDH. 1 hit.
PfamPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsTIGR00169. leuB. 1 hit.
PROSITEPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameLEU3_BRUA2
AccessionPrimary (citable) accession number: Q2YL58
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: February 7, 2006
Last modified: February 9, 2010
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Brucella abortus strain 2308

Brucella abortus (strain 2308): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents