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Protein

Bifunctional protein FolD

Gene

folD

Organism
Brucella abortus (strain 2308)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH.UniRule annotation
5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.UniRule annotation

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei193NADPUniRule annotation1
Binding sitei234NADP; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi168 – 170NADPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Oxidoreductase
Biological processAmino-acid biosynthesis, Histidine biosynthesis, Methionine biosynthesis, One-carbon metabolism, Purine biosynthesis
LigandNADP

Enzyme and pathway databases

UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein FolDUniRule annotation
Including the following 2 domains:
Methylenetetrahydrofolate dehydrogenaseUniRule annotation (EC:1.5.1.5UniRule annotation)
Methenyltetrahydrofolate cyclohydrolaseUniRule annotation (EC:3.5.4.9UniRule annotation)
Gene namesi
Name:folDUniRule annotation
Ordered Locus Names:BAB2_0457
OrganismiBrucella abortus (strain 2308)
Taxonomic identifieri359391 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000002719 Componenti: Chromosome II

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002682941 – 299Bifunctional protein FolDAdd BLAST299

Proteomic databases

PRIDEiQ2YL33.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ2YL33.
SMRiQ2YL33.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000218242.
KOiK01491.
OMAiTRINAGR.

Family and domain databases

HAMAPiMF_01576. THF_DHG_CYH. 1 hit.
InterProiView protein in InterPro
IPR016040. NAD(P)-bd_dom.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020867. THF_DH/CycHdrlase_CS.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
PANTHERiPTHR10025. PTHR10025. 1 hit.
PfamiView protein in Pfam
PF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiView protein in PROSITE
PS00766. THF_DHG_CYH_1. 1 hit.
PS00767. THF_DHG_CYH_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2YL33-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQLIDGKKL AEDVVSTVKT ETEKLVAATG VVPGIAVVIV GEDPASQVYV
60 70 80 90 100
ASKSRKAKEC GFHSVQHDLP ETASEQELLN LIEGLNNDPA IHGILVQLPL
110 120 130 140 150
PGHIDSGRVI QTIAPEKDVD GFHFINVGKL GTGEVETAFV PCTPAGAMIM
160 170 180 190 200
IERVHGRDLS GLNAVVIGRS NIVGKPMFNL LLAANATVTV AHSRTKDLPA
210 220 230 240 250
IARNADILVA AVGRPQMVKG DWVKPGATVI DVGINRIPAP ERGEGKTRLV
260 270 280 290
GDVDFAEAEK VAGAITPVPG GVGPMTIAML MANTLTAACR SAGMKKPVF
Length:299
Mass (Da):31,231
Last modified:February 7, 2006 - v1
Checksum:i7446B7B964FB967A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM040265 Genomic DNA. Translation: CAJ12623.1.
RefSeqiWP_002967255.1. NZ_KN046823.1.

Genome annotation databases

EnsemblBacteriaiCAJ12623; CAJ12623; BAB2_0457.
GeneIDi29594995.
KEGGibmf:BAB2_0457.
PATRICifig|359391.11.peg.2651.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiFOLD_BRUA2
AccessioniPrimary (citable) accession number: Q2YL33
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: February 7, 2006
Last modified: July 5, 2017
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Brucella abortus strain 2308
    Brucella abortus (strain 2308): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families