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Protein

Glutamyl-tRNA(Gln) amidotransferase subunit A

Gene

gatA

Organism
Brucella abortus (strain 2308)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).UniRule annotation

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei79 – 791Charge relay systemUniRule annotation
Active sitei159 – 1591Charge relay systemUniRule annotation
Active sitei183 – 1831Acyl-ester intermediateUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. translation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBABO359391:GKDV-2896-MONOMER.
BMEL359391:GJOQ-2896-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit AUniRule annotation (EC:6.3.5.7UniRule annotation)
Short name:
Glu-ADT subunit AUniRule annotation
Gene namesi
Name:gatAUniRule annotation
Ordered Locus Names:BAB2_0646
OrganismiBrucella abortus (strain 2308)
Taxonomic identifieri359391 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000002719 Componenti: Chromosome II

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 493493Glutamyl-tRNA(Gln) amidotransferase subunit APRO_0000241078Add
BLAST

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.UniRule annotation

Protein-protein interaction databases

STRINGi359391.BAB2_0646.

Family & Domainsi

Sequence similaritiesi

Belongs to the amidase family. GatA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0154.
HOGENOMiHOG000116699.
KOiK02433.
OMAiSKQYRER.
OrthoDBiEOG61P6R9.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2YKL3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSELTALTIA EARDKLKAKA ITATELTDAY LSAIDAANDA INAYVAVTHD
60 70 80 90 100
QARSMAKASD ERIAKGEAGA LEGIPLGVKD LFATKGVHTQ ACSHILDGFK
110 120 130 140 150
PEYESTVTAN LWADGAVMLG KLNMDEVAMG SSNETSYYGP VKNPWRAKGS
160 170 180 190 200
NADLVPGGSS GGSAAAVAAH LCAGATATDT GGSIRQPAAF TGTVGIKPTY
210 220 230 240 250
GRVSRWGTVA FASSLDQAGP IARDVRDAAI LMKSMASLDL KDTTSVDLPV
260 270 280 290 300
PDYEAALGRS VKGMKIGIPR EYRVDGMPGE IEELWQKGIQ YLKDAGAEIV
310 320 330 340 350
DISLPHTKYA LPAYYIVAPA EASSNLARYD GVRYGLRVPG KDIADMYEQT
360 370 380 390 400
RAAGFGKEVK RRIMIGTYVL SAGYYDAYYL RAQKVRTLIK KDFEDVFAKG
410 420 430 440 450
VDAILTPATP SAAFGLADEV LANDPVKMYL NDIFTVTVNM AGLPGIAVPA
460 470 480 490
GLNGQGLPLG LQLIGRPFEE ETLFQAAHVI EQAAGRFTPA KWW
Length:493
Mass (Da):52,399
Last modified:February 7, 2006 - v1
Checksum:i9830C28DEC567BD2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM040265 Genomic DNA. Translation: CAJ12812.1.
RefSeqiYP_418821.1. NC_007624.1.

Genome annotation databases

EnsemblBacteriaiCAJ12812; CAJ12812; BAB2_0646.
KEGGibmf:BAB2_0646.
PATRICi17848835. VBIBruMel86222_2940.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM040265 Genomic DNA. Translation: CAJ12812.1.
RefSeqiYP_418821.1. NC_007624.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi359391.BAB2_0646.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ12812; CAJ12812; BAB2_0646.
KEGGibmf:BAB2_0646.
PATRICi17848835. VBIBruMel86222_2940.

Phylogenomic databases

eggNOGiCOG0154.
HOGENOMiHOG000116699.
KOiK02433.
OMAiSKQYRER.
OrthoDBiEOG61P6R9.

Enzyme and pathway databases

BioCyciBABO359391:GKDV-2896-MONOMER.
BMEL359391:GJOQ-2896-MONOMER.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 2308.

Entry informationi

Entry nameiGATA_BRUA2
AccessioniPrimary (citable) accession number: Q2YKL3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: February 7, 2006
Last modified: April 1, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Brucella abortus strain 2308
    Brucella abortus (strain 2308): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.