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Q2YKK7 (LOVHK_BRUA2) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Blue-light-activated histidine kinase

EC=2.7.13.3
Alternative name(s):
BA-LOV-histidine kinase
Short name=BA-LOV-HK
Gene names
Ordered Locus Names:BAB2_0652
OrganismBrucella abortus (strain 2308) [Complete proteome] [HAMAP]
Taxonomic identifier359391 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length489 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Photosensitive kinase that is involved in increased bacterial virulence upon exposure to light. Once ejected from an infected animal host, sunlight acts as an environmental signal that increases the virulence of the bacterium, preparing it for infection of the next host By similarity. This photoreceptor protein is directly related to the bacterium's survival and replication within host macrophages, as it is required for optimal replication of bacteria inside macrophages. Ref.2

Catalytic activity

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Induction

Induced after invasion of host macrophages. Ref.2

Post-translational modification

FMN binds covalently to cysteine after exposure to blue light and this bond is spontaneously broken in the dark By similarity.

Sequence similarities

Contains 2 PAC (PAS-associated C-terminal) domains.

Contains 1 PAS (PER-ARNT-SIM) domain.

Sequence caution

The sequence CAJ12818.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 489489Blue-light-activated histidine kinase
PRO_0000361282

Regions

Domain19 – 9375PAS
Domain93 – 14755PAC 1
Domain232 – 28150PAC 2
Region259 – 34183HWE histidine kinase domain

Amino acid modifications

Modified residue691S-4a-FMN cysteine By similarity
Modified residue2881Phosphohistidine; by autocatalysis By similarity

Experimental info

Mutagenesis691C → A: Loss of ability to multiply efficiently inside host macrophages. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q2YKK7 [UniParc].

Last modified January 20, 2009. Version 2.
Checksum: 6F2A04467FB26C5B

FASTA48954,874
        10         20         30         40         50         60 
MAIDLRPFIP FGRGALSQAT DPFRAAVEFT LMPMLITNPH LPDNPIVFAN PAFLKLTGYE 

        70         80         90        100        110        120 
ADEVMGRNCR FLQGHGTDPA HVRAIKSAIA AEKPIDIDII NYKKSGEAFW NRLHISPVHN 

       130        140        150        160        170        180 
ANGRLQHFVS SQLDVTLELS RLVELEKERK TLSIETARSK DQLDYIVEVA NIGFWTREFY 

       190        200        210        220        230        240 
SGKMTCSAEC RRIYGFTPDE PVHFDTILDL VVLEDRMTVV QKAHQAVTGE PYSIEYRIVT 

       250        260        270        280        290        300 
RLGETRWLET RAKALTGENP LVLGIVQDVT ERKKAEANKA LVSREIAHRF KNSMAMVQSI 

       310        320        330        340        350        360 
ANQTLRNTYD PEQANRLFSE RLRALSQAHD MLLKENWAGA TIQQICATAL APFNSTFANR 

       370        380        390        400        410        420 
IHMSGPHLLV SDRVTVALSL AFYELATNAV KYGALSNEKG VINITWAIME DKGEKKFHMR 

       430        440        450        460        470        480 
WAESRGPEVM QPARRGFGQR LLHSVLAEEL KAKCDVEFAA SGLLIDVLAP ITPEVFPGMG 


HNVPEQRIA 

« Hide

References

« Hide 'large scale' references
[1]"Whole-genome analyses of speciation events in pathogenic Brucellae."
Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E.
Infect. Immun. 73:8353-8361(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 2308.
[2]"Blue-light-activated histidine kinases: two-component sensors in bacteria."
Swartz T.E., Tseng T.-S., Frederickson M.A., Paris G., Comerci D.J., Rajashekara G., Kim J.-G., Mudgett M.B., Splitter G.A., Ugalde R.A., Goldbaum F.A., Briggs W.R., Bogomolni R.A.
Science 317:1090-1093(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: ROLE IN VIRULENCE, INDUCTION, MUTAGENESIS OF CYS-69.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM040265 Genomic DNA. Translation: CAJ12818.1. Different initiation.
RefSeqYP_418827.1. NC_007624.1.

3D structure databases

ProteinModelPortalQ2YKK7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING359391.BAB2_0652.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAJ12818; CAJ12818; BAB2_0652.
GeneID3827969.
KEGGbmf:BAB2_0652.
PATRIC17848847. VBIBruMel86222_2946.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2202.
HOGENOMHOG000032444.
KOK00936.
OrthoDBEOG6TBHBG.

Enzyme and pathway databases

BioCycBABO359391:GKDV-2902-MONOMER.
BMEL359391:GJOQ-2902-MONOMER.

Family and domain databases

InterProIPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR013655. PAS_fold_3.
IPR011102. Sig_transdc_His_kinase_HWE.
[Graphical view]
PfamPF07536. HWE_HK. 1 hit.
PF08447. PAS_3. 1 hit.
PF13426. PAS_9. 1 hit.
[Graphical view]
SMARTSM00911. HWE_HK. 1 hit.
SM00086. PAC. 2 hits.
SM00091. PAS. 2 hits.
[Graphical view]
SUPFAMSSF55785. SSF55785. 2 hits.
TIGRFAMsTIGR00229. sensory_box. 2 hits.
PROSITEPS50113. PAC. 2 hits.
PS50112. PAS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLOVHK_BRUA2
AccessionPrimary (citable) accession number: Q2YKK7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: January 20, 2009
Last modified: May 14, 2014
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Brucella abortus strain 2308

Brucella abortus (strain 2308): entries and gene names