Blue-light-activated histidine kinase - Brucella abortus (strain 2308)

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Q2YKK7 (LOVHK_BRUA2) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 56. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Blue-light-activated histidine kinase
EC=2.7.13.3

Alternative name(s):
BA-LOV-histidine kinase
Short name=BA-LOV-HK

Gene names

Ordered Locus Names:

BAB2_0652

Organism

Brucella abortus (strain 2308) [Complete proteome] [HAMAP]

Taxonomic identifier

359391 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella ›

Protein attributes

Sequence length	489 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Photosensitive kinase that is involved in increased bacterial virulence upon exposure to light. Once ejected from an infected animal host, sunlight acts as an environmental signal that increases the virulence of the bacterium, preparing it for infection of the next host By similarity. This photoreceptor protein is directly related to the bacterium's survival and replication within host macrophages, as it is required for optimal replication of bacteria inside macrophages. Ref.2
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Induction	Induced after invasion of host macrophages. Ref.2
Post-translational modification	FMN binds covalently to cysteine after exposure to blue light and this bond is spontaneously broken in the dark By similarity.
Sequence similarities	Contains 2 PAC (PAS-associated C-terminal) domains. Contains 1 PAS (PER-ARNT-SIM) domain.
Sequence caution	The sequence CAJ12818.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Sensory transduction Virulence
Domain	Repeat
Ligand	ATP-binding Chromophore FMN Flavoprotein Nucleotide-binding
Molecular function	Kinase Photoreceptor protein Receptor Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW protein-chromophore linkage Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro signal transduction by phosphorylation Inferred from electronic annotation. Source: GOC
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphorelay sensor kinase activity Inferred from electronic annotation. Source: InterPro photoreceptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 489

489

Blue-light-activated histidine kinase

PRO_0000361282

Regions

Domain

19 – 93

PAS

Domain

93 – 147

PAC 1

Domain

232 – 281

PAC 2

Region

259 – 341

HWE histidine kinase domain

Amino acid modifications

Modified residue

S-4a-FMN cysteine By similarity

Modified residue

288

Phosphohistidine; by autocatalysis By similarity

Experimental info

Mutagenesis

C → A: Loss of ability to multiply efficiently inside host macrophages. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

Q2YKK7 [UniParc].

Last modified January 20, 2009. Version 2.
Checksum: 6F2A04467FB26C5B
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FASTA

489

54,874

        10         20         30         40         50         60 
MAIDLRPFIP FGRGALSQAT DPFRAAVEFT LMPMLITNPH LPDNPIVFAN PAFLKLTGYE 

        70         80         90        100        110        120 
ADEVMGRNCR FLQGHGTDPA HVRAIKSAIA AEKPIDIDII NYKKSGEAFW NRLHISPVHN 

       130        140        150        160        170        180 
ANGRLQHFVS SQLDVTLELS RLVELEKERK TLSIETARSK DQLDYIVEVA NIGFWTREFY 

       190        200        210        220        230        240 
SGKMTCSAEC RRIYGFTPDE PVHFDTILDL VVLEDRMTVV QKAHQAVTGE PYSIEYRIVT 

       250        260        270        280        290        300 
RLGETRWLET RAKALTGENP LVLGIVQDVT ERKKAEANKA LVSREIAHRF KNSMAMVQSI 

       310        320        330        340        350        360 
ANQTLRNTYD PEQANRLFSE RLRALSQAHD MLLKENWAGA TIQQICATAL APFNSTFANR 

       370        380        390        400        410        420 
IHMSGPHLLV SDRVTVALSL AFYELATNAV KYGALSNEKG VINITWAIME DKGEKKFHMR 

       430        440        450        460        470        480 
WAESRGPEVM QPARRGFGQR LLHSVLAEEL KAKCDVEFAA SGLLIDVLAP ITPEVFPGMG 


HNVPEQRIA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole-genome analyses of speciation events in pathogenic Brucellae." Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E. Infect. Immun. 73:8353-8361(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 2308.
[2]	"Blue-light-activated histidine kinases: two-component sensors in bacteria." Swartz T.E., Tseng T.-S., Frederickson M.A., Paris G., Comerci D.J., Rajashekara G., Kim J.-G., Mudgett M.B., Splitter G.A., Ugalde R.A., Goldbaum F.A., Briggs W.R., Bogomolni R.A. Science 317:1090-1093(2007) [PubMed] [Europe PMC] [Abstract] Cited for: ROLE IN VIRULENCE, INDUCTION, MUTAGENESIS OF CYS-69.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM040265 Genomic DNA. Translation: CAJ12818.1. Different initiation.
RefSeq	YP_418827.1. NC_007624.1.
3D structure databases
HSSP	HSSP built from PDB template 1VB6 based on UniProtKB P76129.
ProteinModelPortal	Q2YKK7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAJ12818; CAJ12818; BAB2_0652.
GeneID	3827969.
KEGG	bmf:BAB2_0652.
PATRIC	17848847. VBIBruMel86222_2946.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2202.
HOGENOM	HOG000032444.
KO	K00936.
ProtClustDB	CLSK898905.
Enzyme and pathway databases
BioCyc	BMEL359391:GJOQ-2902-MONOMER.
Family and domain databases
InterPro	IPR001610. PAC. IPR000014. PAS. IPR000700. PAS-assoc_C. IPR013655. PAS_fold_3. IPR011102. Sig_transdc_His_kinase_HWE. [Graphical view]
Pfam	PF07536. HWE_HK. 1 hit. PF08447. PAS_3. 1 hit. [Graphical view]
SMART	SM00911. HWE_HK. 1 hit. SM00086. PAC. 2 hits. SM00091. PAS. 2 hits. [Graphical view]
TIGRFAMs	TIGR00229. sensory_box. 2 hits.
PROSITE	PS50113. PAC. 2 hits. PS50112. PAS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LOVHK_BRUA2

Accession

Primary (citable) accession number: Q2YKK7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	January 20, 2009
Last modified:	May 1, 2013
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Brucella abortus strain 2308

Brucella abortus (strain 2308): entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents