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Protein

Blue-light-activated histidine kinase

Gene

BAB2_0652

Organism
Brucella abortus (strain 2308)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Photosensitive kinase that is involved in increased bacterial virulence upon exposure to light. Once ejected from an infected animal host, sunlight acts as an environmental signal that increases the virulence of the bacterium, preparing it for infection of the next host (By similarity). This photoreceptor protein is directly related to the bacterium's survival and replication within host macrophages, as it is required for optimal replication of bacteria inside macrophages.By similarity1 Publication

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Photoreceptor protein, Receptor, Transferase

Keywords - Biological processi

Sensory transduction, Virulence

Keywords - Ligandi

ATP-binding, Chromophore, Flavoprotein, FMN, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Blue-light-activated histidine kinase (EC:2.7.13.3)
Alternative name(s):
BA-LOV-histidine kinase
Short name:
BA-LOV-HK
Gene namesi
Ordered Locus Names:BAB2_0652
OrganismiBrucella abortus (strain 2308)
Taxonomic identifieri359391 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000002719 Componenti: Chromosome II

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi69C → A: Loss of ability to multiply efficiently inside host macrophages. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003612821 – 489Blue-light-activated histidine kinaseAdd BLAST489

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei69S-4a-FMN cysteineBy similarity1
Modified residuei288Phosphohistidine; by autocatalysisBy similarity1

Post-translational modificationi

FMN binds covalently to cysteine after exposure to blue light and this bond is spontaneously broken in the dark.By similarity

Keywords - PTMi

Phosphoprotein

Expressioni

Inductioni

Induced after invasion of host macrophages.1 Publication

Structurei

Secondary structure

1489
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi270 – 304Combined sources35
Beta strandi305 – 307Combined sources3
Helixi311 – 333Combined sources23
Turni334 – 336Combined sources3
Helixi342 – 356Combined sources15
Beta strandi360 – 364Combined sources5
Helixi372 – 391Combined sources20
Beta strandi396 – 399Combined sources4
Beta strandi401 – 427Combined sources27
Helixi437 – 444Combined sources8
Helixi446 – 450Combined sources5
Beta strandi453 – 459Combined sources7
Beta strandi462 – 470Combined sources9
Turni473 – 475Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5EPVX-ray2.51A/B/C/D266-489[»]
ProteinModelPortaliQ2YKK7.
SMRiQ2YKK7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 93PASPROSITE-ProRule annotationAdd BLAST75
Domaini93 – 147PAC 1PROSITE-ProRule annotationAdd BLAST55
Domaini232 – 281PAC 2PROSITE-ProRule annotationAdd BLAST50

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni259 – 341HWE histidine kinase domainAdd BLAST83

Sequence similaritiesi

Contains 2 PAC (PAS-associated C-terminal) domains.PROSITE-ProRule annotation
Contains 1 PAS (PER-ARNT-SIM) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000032444.

Family and domain databases

InterProiIPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR013655. PAS_fold_3.
IPR011102. Sig_transdc_His_kinase_HWE.
[Graphical view]
PfamiPF07536. HWE_HK. 1 hit.
PF08447. PAS_3. 1 hit.
PF13426. PAS_9. 1 hit.
[Graphical view]
SMARTiSM00911. HWE_HK. 1 hit.
SM00086. PAC. 2 hits.
SM00091. PAS. 2 hits.
[Graphical view]
SUPFAMiSSF55785. SSF55785. 2 hits.
TIGRFAMsiTIGR00229. sensory_box. 2 hits.
PROSITEiPS50113. PAC. 2 hits.
PS50112. PAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2YKK7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIDLRPFIP FGRGALSQAT DPFRAAVEFT LMPMLITNPH LPDNPIVFAN
60 70 80 90 100
PAFLKLTGYE ADEVMGRNCR FLQGHGTDPA HVRAIKSAIA AEKPIDIDII
110 120 130 140 150
NYKKSGEAFW NRLHISPVHN ANGRLQHFVS SQLDVTLELS RLVELEKERK
160 170 180 190 200
TLSIETARSK DQLDYIVEVA NIGFWTREFY SGKMTCSAEC RRIYGFTPDE
210 220 230 240 250
PVHFDTILDL VVLEDRMTVV QKAHQAVTGE PYSIEYRIVT RLGETRWLET
260 270 280 290 300
RAKALTGENP LVLGIVQDVT ERKKAEANKA LVSREIAHRF KNSMAMVQSI
310 320 330 340 350
ANQTLRNTYD PEQANRLFSE RLRALSQAHD MLLKENWAGA TIQQICATAL
360 370 380 390 400
APFNSTFANR IHMSGPHLLV SDRVTVALSL AFYELATNAV KYGALSNEKG
410 420 430 440 450
VINITWAIME DKGEKKFHMR WAESRGPEVM QPARRGFGQR LLHSVLAEEL
460 470 480
KAKCDVEFAA SGLLIDVLAP ITPEVFPGMG HNVPEQRIA
Length:489
Mass (Da):54,874
Last modified:January 20, 2009 - v2
Checksum:i6F2A04467FB26C5B
GO

Sequence cautioni

The sequence CAJ12818 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM040265 Genomic DNA. Translation: CAJ12818.1. Different initiation.
RefSeqiWP_002971240.1. NZ_KN046823.1.

Genome annotation databases

EnsemblBacteriaiCAJ12818; CAJ12818; BAB2_0652.
GeneIDi3827969.
KEGGibmf:BAB2_0652.
PATRICi17848847. VBIBruMel86222_2946.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM040265 Genomic DNA. Translation: CAJ12818.1. Different initiation.
RefSeqiWP_002971240.1. NZ_KN046823.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5EPVX-ray2.51A/B/C/D266-489[»]
ProteinModelPortaliQ2YKK7.
SMRiQ2YKK7.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ12818; CAJ12818; BAB2_0652.
GeneIDi3827969.
KEGGibmf:BAB2_0652.
PATRICi17848847. VBIBruMel86222_2946.

Phylogenomic databases

HOGENOMiHOG000032444.

Family and domain databases

InterProiIPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR013655. PAS_fold_3.
IPR011102. Sig_transdc_His_kinase_HWE.
[Graphical view]
PfamiPF07536. HWE_HK. 1 hit.
PF08447. PAS_3. 1 hit.
PF13426. PAS_9. 1 hit.
[Graphical view]
SMARTiSM00911. HWE_HK. 1 hit.
SM00086. PAC. 2 hits.
SM00091. PAS. 2 hits.
[Graphical view]
SUPFAMiSSF55785. SSF55785. 2 hits.
TIGRFAMsiTIGR00229. sensory_box. 2 hits.
PROSITEiPS50113. PAC. 2 hits.
PS50112. PAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLOVHK_BRUA2
AccessioniPrimary (citable) accession number: Q2YKK7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: January 20, 2009
Last modified: November 2, 2016
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Brucella abortus strain 2308
    Brucella abortus (strain 2308): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.