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Q2YKB8

- BIOB_BRUA2

UniProt

Q2YKB8 - BIOB_BRUA2

Protein

Biotin synthase

Gene

bioB

Organism
Brucella abortus (strain 2308)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (20 Dec 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi63 – 631Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi67 – 671Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi70 – 701Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi107 – 1071Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi138 – 1381Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi198 – 1981Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi270 – 2701Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciBABO359391:GKDV-2996-MONOMER.
    BMEL359391:GJOQ-2996-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:BAB2_0744
    OrganismiBrucella abortus (strain 2308)
    Taxonomic identifieri359391 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
    ProteomesiUP000002719: Chromosome II

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 328328Biotin synthasePRO_0000381248Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi359391.BAB2_0744.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2YKB8.
    SMRiQ2YKB8. Positions 15-322.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239957.
    KOiK01012.
    OMAiRIMMPAS.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q2YKB8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPDRGGENGA SCSVGRWSAE EARAIYNLPF NDLLFRAHGL HRENFDPNRI    50
    QLSKLLNIKT GGCPEDCGYC SQSASAENGL KASKLMEIET VLEEARKAKA 100
    AGATRYCMGA AWRSPKDRDM PALTHMIESV KAMGLETCMT LGMLDSDKAE 150
    KLADAGLDYY NHNIDTSERF YPAVITTRSF EDRLDTLANV RNAGIKVCSG 200
    GILGLGEEAE DRIDMLVTLA NLPEPPESVP INMLIPMPGT RLAKAAPVDP 250
    LEFVRVVALA RILMPKSHVR LTAGRTAMSD EMQALCFFAG ANSLFMGDTL 300
    LTAANPGDDR DSSLLRRLGI QAETEQPA 328
    Length:328
    Mass (Da):35,653
    Last modified:December 20, 2005 - v1
    Checksum:iB3936726D01DAB70
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM040265 Genomic DNA. Translation: CAJ12910.1.
    RefSeqiYP_418916.1. NC_007624.1.

    Genome annotation databases

    EnsemblBacteriaiCAJ12910; CAJ12910; BAB2_0744.
    GeneIDi3828135.
    KEGGibmf:BAB2_0744.
    PATRICi17849038. VBIBruMel86222_3039.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM040265 Genomic DNA. Translation: CAJ12910.1 .
    RefSeqi YP_418916.1. NC_007624.1.

    3D structure databases

    ProteinModelPortali Q2YKB8.
    SMRi Q2YKB8. Positions 15-322.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 359391.BAB2_0744.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAJ12910 ; CAJ12910 ; BAB2_0744 .
    GeneIDi 3828135.
    KEGGi bmf:BAB2_0744.
    PATRICi 17849038. VBIBruMel86222_3039.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239957.
    KOi K01012.
    OMAi RIMMPAS.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci BABO359391:GKDV-2996-MONOMER.
    BMEL359391:GJOQ-2996-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 2308.

    Entry informationi

    Entry nameiBIOB_BRUA2
    AccessioniPrimary (citable) accession number: Q2YKB8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: December 20, 2005
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Brucella abortus strain 2308
      Brucella abortus (strain 2308): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3