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Q2YJB2

- BHUA_BRUA2

UniProt

Q2YJB2 - BHUA_BRUA2

Protein

Heme transporter BhuA

Gene

bhuA

Organism
Brucella abortus (strain 2308)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (20 Dec 2005)
      Previous versions | rss
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    Functioni

    Heme transporter playing an important role in stationary-phase iron acquisition and required for maintenance of chronic infection in mice.1 Publication

    GO - Molecular functioni

    1. receptor activity Source: InterPro
    2. transporter activity Source: InterPro

    Keywords - Molecular functioni

    Receptor

    Enzyme and pathway databases

    BioCyciBABO359391:GKDV-3412-MONOMER.
    BMEL359391:GJOQ-3412-MONOMER.

    Protein family/group databases

    TCDBi1.B.14.2.10. the outer membrane receptor (omr) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Heme transporter BhuA
    Alternative name(s):
    Brucella heme uptake protein A
    Gene namesi
    Name:bhuA
    Ordered Locus Names:BAB2_1150
    OrganismiBrucella abortus (strain 2308)
    Taxonomic identifieri359391 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
    ProteomesiUP000002719: Chromosome II

    Subcellular locationi

    Cell outer membrane By similarity

    GO - Cellular componenti

    1. cell outer membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell outer membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    The mutant is unable to use hemin as an iron source but retains the ability to use ferric chloride and the siderophore 2,3-DHBA. Exhibits an accelerated loss of viability after 144 hours of culture in low-iron minimal medium. Exhibits significant attenuation in murine macrophages and is unable to maintain a chronic spleen infection in mice.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 661638Heme transporter BhuAPRO_0000325857Add
    BLAST

    Expressioni

    Inductioni

    Induced in absence of iron. Maximally expressed during stationary phase when cultivated in low-iron minimal medium.

    Interactioni

    Protein-protein interaction databases

    STRINGi359391.BAB2_1150.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the TonB-dependent receptor family.Curated

    Keywords - Domaini

    Signal, TonB box, Transmembrane, Transmembrane beta strand

    Phylogenomic databases

    eggNOGiCOG1629.
    HOGENOMiHOG000143472.
    KOiK16087.
    OMAiDPVWAPG.
    OrthoDBiEOG6JQGXW.

    Family and domain databases

    Gene3Di2.170.130.10. 1 hit.
    2.40.170.20. 1 hit.
    InterProiIPR012910. Plug.
    IPR000531. TonB-dep_rcpt_b-brl.
    [Graphical view]
    PfamiPF07715. Plug. 1 hit.
    PF00593. TonB_dep_Rec. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q2YJB2-1 [UniParc]FASTAAdd to Basket

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    MKFTRTLVLV STSLLATVAT SQAQEVKRDT KKQGEVVLKP ITIISHGKDN    50
    IEATGGTVLT YKDIEKLQPA NVSELFSRQS SIAVSGGGGP SKRIHVLGME 100
    QSNLAVSVDG VPQTATSWHH TGSNVIDPAF LKRVEVEAGA AAADSGFGAA 150
    AGAIRYETVN ALDLLEPGKT FGARIIGSYG TNGRGFSGST AAYGLKDGFD 200
    WLLMLHGTSG HNYKNGDGTE ILGTEPAARN ILGKAGYEFD GNRIDIGYER 250
    SRDKADRLIK MNMGLPGDTE YPLEVARDSV NIKYTRTDAT DMWDPEVQFY 300
    YNRNDYWRND YQNRTNGNMI LKEDLYGGKL QNTFTIDYGK ITAGIDFGKH 350
    DYNTDNYGHN DRRYRKFNTQ QVGAFTQGRF EFDNGFSLST GARYDYSRFA 400
    DWNDEVFSDS GASVNGTLSY KFNEHIEVFA GASRTWLGYV LGDYGYVHAR 450
    NNAFYTDPTF SPGRARNYKA GVNFGGADWS AGITLFDTRI AGLPNYDSQK 500
    LGNDPEEYRS RGFTLNARYI WNYTTIGATF TKAKVTAGDD PVLPNSGSFM 550
    PIGDMATLFI DQEIPDYNMK VGATLAWAGR ISDEAATAAN FYDQPAYTVV 600
    NAYAEWNPPA VKNMTLRVGV ENLFNENYYE RTSFAPSQNR GGIDAVWAPG 650
    RTFTFQTAFK F 661
    Length:661
    Mass (Da):72,931
    Last modified:December 20, 2005 - v1
    Checksum:i4EF2DE0C6CE74C7B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM040265 Genomic DNA. Translation: CAJ13316.1.
    RefSeqiYP_419272.1. NC_007624.1.

    Genome annotation databases

    EnsemblBacteriaiCAJ13316; CAJ13316; BAB2_1150.
    GeneIDi3828017.
    KEGGibmf:BAB2_1150.
    PATRICi17849895. VBIBruMel86222_3455.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM040265 Genomic DNA. Translation: CAJ13316.1 .
    RefSeqi YP_419272.1. NC_007624.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 359391.BAB2_1150.

    Protein family/group databases

    TCDBi 1.B.14.2.10. the outer membrane receptor (omr) family.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAJ13316 ; CAJ13316 ; BAB2_1150 .
    GeneIDi 3828017.
    KEGGi bmf:BAB2_1150.
    PATRICi 17849895. VBIBruMel86222_3455.

    Phylogenomic databases

    eggNOGi COG1629.
    HOGENOMi HOG000143472.
    KOi K16087.
    OMAi DPVWAPG.
    OrthoDBi EOG6JQGXW.

    Enzyme and pathway databases

    BioCyci BABO359391:GKDV-3412-MONOMER.
    BMEL359391:GJOQ-3412-MONOMER.

    Family and domain databases

    Gene3Di 2.170.130.10. 1 hit.
    2.40.170.20. 1 hit.
    InterProi IPR012910. Plug.
    IPR000531. TonB-dep_rcpt_b-brl.
    [Graphical view ]
    Pfami PF07715. Plug. 1 hit.
    PF00593. TonB_dep_Rec. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 2308.
    2. "Brucella abortus requires the heme transporter BhuA for maintenance of chronic infection in BALB/c mice."
      Paulley J.T., Anderson E.S., Roop R.M. II
      Infect. Immun. 75:5248-5254(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN HEME TRANSPORT, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiBHUA_BRUA2
    AccessioniPrimary (citable) accession number: Q2YJB2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: December 20, 2005
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Brucella abortus strain 2308
      Brucella abortus (strain 2308): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3