Q2YJB2 (BHUA_BRUA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Heme transporter BhuA

Alternative name(s):
Brucella heme uptake protein A

Gene names

Name:	bhuA
Ordered Locus Names:	BAB2_1150

Organism

Brucella abortus (strain 2308) [Complete proteome] [HAMAP]

Taxonomic identifier

359391 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella abortus

Protein attributes

Sequence length	661 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Heme transporter playing an important role in stationary-phase iron acquisition and required for maintenance of chronic infection in mice. Ref.2
Subcellular location	Cell outer membrane By similarity.
Induction	Induced in absence of iron. Maximally expressed during stationary phase when cultivated in low-iron minimal medium.
Disruption phenotype	The mutant is unable to use hemin as an iron source but retains the ability to use ferric chloride and the siderophore 2,3-DHBA. Exhibits an accelerated loss of viability after 144 hours of culture in low-iron minimal medium. Exhibits significant attenuation in murine macrophages and is unable to maintain a chronic spleen infection in mice. Ref.2
Sequence similarities	Belongs to the TonB-dependent receptor family.

Ontologies

Keywords
Cellular component	Cell membrane Cell outer membrane Membrane
Domain	Signal TonB box Transmembrane Transmembrane beta strand
Molecular function	Receptor
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	cell outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	receptor activity Inferred from electronic annotation. Source: InterPro transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 661	638	Heme transporter BhuA		PRO_0000325857

Sequences

	Sequence		Length	Mass (Da)
	Q2YJB2 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 4EF2DE0C6CE74C7B	FASTA	661	72,931
	10 20 30 40 50 60 MKFTRTLVLV STSLLATVAT SQAQEVKRDT KKQGEVVLKP ITIISHGKDN IEATGGTVLT 70 80 90 100 110 120 YKDIEKLQPA NVSELFSRQS SIAVSGGGGP SKRIHVLGME QSNLAVSVDG VPQTATSWHH 130 140 150 160 170 180 TGSNVIDPAF LKRVEVEAGA AAADSGFGAA AGAIRYETVN ALDLLEPGKT FGARIIGSYG 190 200 210 220 230 240 TNGRGFSGST AAYGLKDGFD WLLMLHGTSG HNYKNGDGTE ILGTEPAARN ILGKAGYEFD 250 260 270 280 290 300 GNRIDIGYER SRDKADRLIK MNMGLPGDTE YPLEVARDSV NIKYTRTDAT DMWDPEVQFY 310 320 330 340 350 360 YNRNDYWRND YQNRTNGNMI LKEDLYGGKL QNTFTIDYGK ITAGIDFGKH DYNTDNYGHN 370 380 390 400 410 420 DRRYRKFNTQ QVGAFTQGRF EFDNGFSLST GARYDYSRFA DWNDEVFSDS GASVNGTLSY 430 440 450 460 470 480 KFNEHIEVFA GASRTWLGYV LGDYGYVHAR NNAFYTDPTF SPGRARNYKA GVNFGGADWS 490 500 510 520 530 540 AGITLFDTRI AGLPNYDSQK LGNDPEEYRS RGFTLNARYI WNYTTIGATF TKAKVTAGDD 550 560 570 580 590 600 PVLPNSGSFM PIGDMATLFI DQEIPDYNMK VGATLAWAGR ISDEAATAAN FYDQPAYTVV 610 620 630 640 650 660 NAYAEWNPPA VKNMTLRVGV ENLFNENYYE RTSFAPSQNR GGIDAVWAPG RTFTFQTAFK F

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole-genome analyses of speciation events in pathogenic Brucellae." Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E. Infect. Immun. 73:8353-8361(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 2308.
[2]	"Brucella abortus requires the heme transporter BhuA for maintenance of chronic infection in BALB/c mice." Paulley J.T., Anderson E.S., Roop R.M. II Infect. Immun. 75:5248-5254(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN HEME TRANSPORT, DISRUPTION PHENOTYPE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM040265 Genomic DNA. Translation: CAJ13316.1.
RefSeq	YP_419272.1. NC_007624.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	359391.BAB2_1150.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAJ13316; CAJ13316; BAB2_1150.
GeneID	3828017.
KEGG	bmf:BAB2_1150.
PATRIC	17849895. VBIBruMel86222_3455.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1629.
HOGENOM	HOG000143472.
KO	K16087.
OMA	DPVWAPG.
ProtClustDB	CLSK898692.
Enzyme and pathway databases
BioCyc	BMEL359391:GJOQ-3412-MONOMER.
Family and domain databases
Gene3D	2.170.130.10. 1 hit. 2.40.170.20. 1 hit.
InterPro	IPR012910. Plug. IPR000531. TonB-dep_rcpt_b-brl. [Graphical view]
Pfam	PF07715. Plug. 1 hit. PF00593. TonB_dep_Rec. 1 hit. [Graphical view]
PROSITE	PS00430. TONB_DEPENDENT_REC_1. False negative. PS01156. TONB_DEPENDENT_REC_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BHUA_BRUA2

Accession

Primary (citable) accession number: Q2YJB2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	December 20, 2005
Last modified:	May 1, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program