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Protein

Heme transporter BhuA

Gene

bhuA

Organism
Brucella abortus (strain 2308)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Heme transporter playing an important role in stationary-phase iron acquisition and required for maintenance of chronic infection in mice.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Receptor

Enzyme and pathway databases

BioCyciBABO359391:GKDV-3412-MONOMER.
BMEL359391:GJOQ-3412-MONOMER.

Protein family/group databases

TCDBi1.B.14.2.10. the outer membrane receptor (omr) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Heme transporter BhuA
Alternative name(s):
Brucella heme uptake protein A
Gene namesi
Name:bhuA
Ordered Locus Names:BAB2_1150
OrganismiBrucella abortus (strain 2308)
Taxonomic identifieri359391 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000002719 Componenti: Chromosome II

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

The mutant is unable to use hemin as an iron source but retains the ability to use ferric chloride and the siderophore 2,3-DHBA. Exhibits an accelerated loss of viability after 144 hours of culture in low-iron minimal medium. Exhibits significant attenuation in murine macrophages and is unable to maintain a chronic spleen infection in mice.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 661638Heme transporter BhuAPRO_0000325857Add
BLAST

Expressioni

Inductioni

Induced in absence of iron. Maximally expressed during stationary phase when cultivated in low-iron minimal medium.

Family & Domainsi

Sequence similaritiesi

Belongs to the TonB-dependent receptor family.Curated

Keywords - Domaini

Signal, TonB box, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiCOG1629.
HOGENOMiHOG000143472.
KOiK16087.
OMAiDPVWAPG.
OrthoDBiEOG6JQGXW.

Family and domain databases

Gene3Di2.170.130.10. 1 hit.
2.40.170.20. 1 hit.
InterProiIPR012910. Plug.
IPR000531. TonB-dep_rcpt_b-brl.
[Graphical view]
PfamiPF07715. Plug. 1 hit.
PF00593. TonB_dep_Rec. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2YJB2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFTRTLVLV STSLLATVAT SQAQEVKRDT KKQGEVVLKP ITIISHGKDN
60 70 80 90 100
IEATGGTVLT YKDIEKLQPA NVSELFSRQS SIAVSGGGGP SKRIHVLGME
110 120 130 140 150
QSNLAVSVDG VPQTATSWHH TGSNVIDPAF LKRVEVEAGA AAADSGFGAA
160 170 180 190 200
AGAIRYETVN ALDLLEPGKT FGARIIGSYG TNGRGFSGST AAYGLKDGFD
210 220 230 240 250
WLLMLHGTSG HNYKNGDGTE ILGTEPAARN ILGKAGYEFD GNRIDIGYER
260 270 280 290 300
SRDKADRLIK MNMGLPGDTE YPLEVARDSV NIKYTRTDAT DMWDPEVQFY
310 320 330 340 350
YNRNDYWRND YQNRTNGNMI LKEDLYGGKL QNTFTIDYGK ITAGIDFGKH
360 370 380 390 400
DYNTDNYGHN DRRYRKFNTQ QVGAFTQGRF EFDNGFSLST GARYDYSRFA
410 420 430 440 450
DWNDEVFSDS GASVNGTLSY KFNEHIEVFA GASRTWLGYV LGDYGYVHAR
460 470 480 490 500
NNAFYTDPTF SPGRARNYKA GVNFGGADWS AGITLFDTRI AGLPNYDSQK
510 520 530 540 550
LGNDPEEYRS RGFTLNARYI WNYTTIGATF TKAKVTAGDD PVLPNSGSFM
560 570 580 590 600
PIGDMATLFI DQEIPDYNMK VGATLAWAGR ISDEAATAAN FYDQPAYTVV
610 620 630 640 650
NAYAEWNPPA VKNMTLRVGV ENLFNENYYE RTSFAPSQNR GGIDAVWAPG
660
RTFTFQTAFK F
Length:661
Mass (Da):72,931
Last modified:December 20, 2005 - v1
Checksum:i4EF2DE0C6CE74C7B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM040265 Genomic DNA. Translation: CAJ13316.1.
RefSeqiWP_006099340.1. NZ_KN046823.1.
YP_419272.1. NC_007624.1.

Genome annotation databases

EnsemblBacteriaiCAJ13316; CAJ13316; BAB2_1150.
GeneIDi3828017.
KEGGibmf:BAB2_1150.
PATRICi17849895. VBIBruMel86222_3455.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM040265 Genomic DNA. Translation: CAJ13316.1.
RefSeqiWP_006099340.1. NZ_KN046823.1.
YP_419272.1. NC_007624.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi1.B.14.2.10. the outer membrane receptor (omr) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ13316; CAJ13316; BAB2_1150.
GeneIDi3828017.
KEGGibmf:BAB2_1150.
PATRICi17849895. VBIBruMel86222_3455.

Phylogenomic databases

eggNOGiCOG1629.
HOGENOMiHOG000143472.
KOiK16087.
OMAiDPVWAPG.
OrthoDBiEOG6JQGXW.

Enzyme and pathway databases

BioCyciBABO359391:GKDV-3412-MONOMER.
BMEL359391:GJOQ-3412-MONOMER.

Family and domain databases

Gene3Di2.170.130.10. 1 hit.
2.40.170.20. 1 hit.
InterProiIPR012910. Plug.
IPR000531. TonB-dep_rcpt_b-brl.
[Graphical view]
PfamiPF07715. Plug. 1 hit.
PF00593. TonB_dep_Rec. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 2308.
  2. "Brucella abortus requires the heme transporter BhuA for maintenance of chronic infection in BALB/c mice."
    Paulley J.T., Anderson E.S., Roop R.M. II
    Infect. Immun. 75:5248-5254(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN HEME TRANSPORT, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiBHUA_BRUA2
AccessioniPrimary (citable) accession number: Q2YJB2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: December 20, 2005
Last modified: June 24, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Brucella abortus strain 2308
    Brucella abortus (strain 2308): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.