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Q2YJB2 (BHUA_BRUA2) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Heme transporter BhuA
Alternative name(s):
Brucella heme uptake protein A
Gene names
Name:bhuA
Ordered Locus Names:BAB2_1150
OrganismBrucella abortus (strain 2308) [Complete proteome] [HAMAP]
Taxonomic identifier359391 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length661 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Heme transporter playing an important role in stationary-phase iron acquisition and required for maintenance of chronic infection in mice. Ref.2

Subcellular location

Cell outer membrane By similarity.

Induction

Induced in absence of iron. Maximally expressed during stationary phase when cultivated in low-iron minimal medium.

Disruption phenotype

The mutant is unable to use hemin as an iron source but retains the ability to use ferric chloride and the siderophore 2,3-DHBA. Exhibits an accelerated loss of viability after 144 hours of culture in low-iron minimal medium. Exhibits significant attenuation in murine macrophages and is unable to maintain a chronic spleen infection in mice. Ref.2

Sequence similarities

Belongs to the TonB-dependent receptor family.

Ontologies

Keywords
   Cellular componentCell outer membrane
Membrane
   DomainSignal
TonB box
Transmembrane
Transmembrane beta strand
   Molecular functionReceptor
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcell outer membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionreceptor activity

Inferred from electronic annotation. Source: InterPro

transporter activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 661638Heme transporter BhuA
PRO_0000325857

Sequences

Sequence LengthMass (Da)Tools
Q2YJB2 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: 4EF2DE0C6CE74C7B

FASTA66172,931
        10         20         30         40         50         60 
MKFTRTLVLV STSLLATVAT SQAQEVKRDT KKQGEVVLKP ITIISHGKDN IEATGGTVLT 

        70         80         90        100        110        120 
YKDIEKLQPA NVSELFSRQS SIAVSGGGGP SKRIHVLGME QSNLAVSVDG VPQTATSWHH 

       130        140        150        160        170        180 
TGSNVIDPAF LKRVEVEAGA AAADSGFGAA AGAIRYETVN ALDLLEPGKT FGARIIGSYG 

       190        200        210        220        230        240 
TNGRGFSGST AAYGLKDGFD WLLMLHGTSG HNYKNGDGTE ILGTEPAARN ILGKAGYEFD 

       250        260        270        280        290        300 
GNRIDIGYER SRDKADRLIK MNMGLPGDTE YPLEVARDSV NIKYTRTDAT DMWDPEVQFY 

       310        320        330        340        350        360 
YNRNDYWRND YQNRTNGNMI LKEDLYGGKL QNTFTIDYGK ITAGIDFGKH DYNTDNYGHN 

       370        380        390        400        410        420 
DRRYRKFNTQ QVGAFTQGRF EFDNGFSLST GARYDYSRFA DWNDEVFSDS GASVNGTLSY 

       430        440        450        460        470        480 
KFNEHIEVFA GASRTWLGYV LGDYGYVHAR NNAFYTDPTF SPGRARNYKA GVNFGGADWS 

       490        500        510        520        530        540 
AGITLFDTRI AGLPNYDSQK LGNDPEEYRS RGFTLNARYI WNYTTIGATF TKAKVTAGDD 

       550        560        570        580        590        600 
PVLPNSGSFM PIGDMATLFI DQEIPDYNMK VGATLAWAGR ISDEAATAAN FYDQPAYTVV 

       610        620        630        640        650        660 
NAYAEWNPPA VKNMTLRVGV ENLFNENYYE RTSFAPSQNR GGIDAVWAPG RTFTFQTAFK 


F 

« Hide

References

« Hide 'large scale' references
[1]"Whole-genome analyses of speciation events in pathogenic Brucellae."
Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E.
Infect. Immun. 73:8353-8361(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 2308.
[2]"Brucella abortus requires the heme transporter BhuA for maintenance of chronic infection in BALB/c mice."
Paulley J.T., Anderson E.S., Roop R.M. II
Infect. Immun. 75:5248-5254(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN HEME TRANSPORT, DISRUPTION PHENOTYPE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM040265 Genomic DNA. Translation: CAJ13316.1.
RefSeqYP_419272.1. NC_007624.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING359391.BAB2_1150.

Protein family/group databases

TCDB1.B.14.2.10. the outer membrane receptor (omr) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAJ13316; CAJ13316; BAB2_1150.
GeneID3828017.
KEGGbmf:BAB2_1150.
PATRIC17849895. VBIBruMel86222_3455.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1629.
HOGENOMHOG000143472.
KOK16087.
OMADPVWAPG.
OrthoDBEOG6JQGXW.
ProtClustDBCLSK898692.

Enzyme and pathway databases

BioCycBABO359391:GKDV-3412-MONOMER.
BMEL359391:GJOQ-3412-MONOMER.

Family and domain databases

Gene3D2.170.130.10. 1 hit.
2.40.170.20. 1 hit.
InterProIPR012910. Plug.
IPR000531. TonB-dep_rcpt_b-brl.
[Graphical view]
PfamPF07715. Plug. 1 hit.
PF00593. TonB_dep_Rec. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBHUA_BRUA2
AccessionPrimary (citable) accession number: Q2YJB2
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: December 20, 2005
Last modified: February 19, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Brucella abortus strain 2308

Brucella abortus (strain 2308): entries and gene names