Q2YIT8 (VIRB4_BRUA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type IV secretion system protein virB4

Gene names

Name:	virB4
Ordered Locus Names:	BAB2_0065

Organism

Brucella abortus (strain 2308) [Complete proteome] [HAMAP]

Taxonomic identifier

359391 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella abortus

Protein attributes

Sequence length	831 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The virB operon is essential for intracellular survival and is not involved in the invasion process. Constitutes a major determinant of virulence in mice. Ref.1
Miscellaneous	Transcription is turned on at the beginning of the stationary phase of vegetative growth.
Sequence similarities	Belongs to the TrbE/VirB4 family.

Ontologies

Keywords
Biological process	Virulence
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 831	831	Type IV secretion system protein virB4		PRO_0000290168
Regions
	Nucleotide binding	457 – 464	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q2YIT8 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 657AAA319B4CC8E5	FASTA	831	94,572
	10 20 30 40 50 60 MGAQSKYAQQ LNNERSLAPF IPFRSQVGPT TVITRDGDFV RTWRIAGLAF ETQDKEKLLI 70 80 90 100 110 120 RKDQLNTLFR AIASNNVALW SHNVRRRTWD HLKSFFSNPF CDALDKKYYG SFSGYRMMSN 130 140 150 160 170 180 ELYLTVIYRP VPAKISRLFN VAVHRSHAEI LQEQQLAIRK LDEIGNQIET SLRRYGGDDG 190 200 210 220 230 240 RGIEVLSTYE DKHGALCSQQ LEFYNFLLSG EWQKVRVPSC PLDEYLGTGW VYAGTETIEI 250 260 270 280 290 300 RTANATRYAR GIDFKDYANH TEPGILNGLM YSDYEYVITQ SFSFMTKRDG KEFLTRQKQR 310 320 330 340 350 360 LQNTEDGSAS QIMEMDIAID QLGRGDFVMG EYHYSLLVFA EDMETVRHNT SHAMNILQDN 370 380 390 400 410 420 GFLATVIATA TDAAFYAQLP CNWRYRPRVA GLTSLNFAGL SCFHNFRAGK RDGNPWGQAL 430 440 450 460 470 480 TLLKTPSGQP AYLNFHYSKG DEDNFDKKLL GNTRIIGQSG AGKTVLMNFC LAQAQKYLHN 490 500 510 520 530 540 APMGMCNVFF DKDQGAKGTI LAIGGKYLAI RNGEPTGFNP FQMEPTAGNI LFLEKLVQVL 550 560 570 580 590 600 VSRDGQHVTT TDESRISHAI RTVMRMRPEL RRLSTVLQNV TEGSDRQDRE NSVAKRLAKW 610 620 630 640 650 660 CFDDGTGKRG TFWWVLDCPQ DQIDFNTHSN YGFDGTDFLD NADVRTPISM YLLHRMELAI 670 680 690 700 710 720 DGRRFIYWMD EAWKWVDDEA FSEFANNKQL TIRKQNGLGV FATQMPSSLL NSKVASALVQ 730 740 750 760 770 780 QVATEIYLPN PKADYHEYTD GFKVTNEEFD IIRSMSEESR MFLVKQGHHS MICRLELNGF 790 800 810 820 830 DDELAILSGS SDNNELLDQV IAEVGDDPSV WLPVFQERRK ARIASSKSTG R

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A homologue of an operon required for DNA transfer in Agrobacterium is required in Brucella abortus for virulence and intracellular multiplication." Sieira R., Comerci D.J., Sanchez D.O., Ugalde R.A. J. Bacteriol. 182:4849-4855(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TRANSCRIPTION, FUNCTION.
[2]	"Whole-genome analyses of speciation events in pathogenic Brucellae." Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E. Infect. Immun. 73:8353-8361(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 2308.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF226278 Genomic DNA. Translation: AAF73897.1. AM040265 Genomic DNA. Translation: CAJ12231.1.
RefSeq	YP_418310.1. NC_007624.1.
3D structure databases
ProteinModelPortal	Q2YIT8.
ModBase	Search...
Protein-protein interaction databases
STRING	359391.BAB2_0065.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAJ12231; CAJ12231; BAB2_0065.
GeneID	3827979.
KEGG	bmf:BAB2_0065.
PATRIC	17847615. VBIBruMel86222_2341.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3451.
HOGENOM	HOG000119263.
KO	K03199.
OMA	FKDYASH.
ProtClustDB	CLSK884268.
Enzyme and pathway databases
BioCyc	BMEL359391:GJOQ-2308-MONOMER.
Family and domain databases
InterPro	IPR004346. CagE_TrbE_VirB. IPR018145. CagE_TrbE_VirB_cntrl_dom. IPR027417. P-loop_NTPase. [Graphical view]
Pfam	PF03135. CagE_TrbE_VirB. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
TIGRFAMs	TIGR00929. VirB4_CagE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

VIRB4_BRUA2

Accession

Primary (citable) accession number: Q2YIT8
Secondary accession number(s): Q57A17, Q9KIS9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 12, 2007
Last sequence update:	February 7, 2006
Last modified:	May 29, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program