Reviewed, UniProtKB/Swiss-Prot Q2YIS9 (HEMH_BRUA2)

Names and origin

Protein names

Recommended name:
    Ferrochelatase
    EC=4.99.1.1
Alternative name(s):
    Protoheme ferro-lyase
    Heme synthetase

Gene names

Name:	hemH
Ordered Locus Names:	BAB2_0075

Organism

Brucella abortus (strain 2308) [Complete proteome] [HAMAP]

Taxonomic identifier

359391 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX. HAMAP MF_00323
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+. HAMAP MF_00323
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. HAMAP MF_00323
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 352	352	Ferrochelatase HAMAP MF_00323		PRO_1000019274
Sites
	Metal binding	222	1	Iron By similarity
	Metal binding	303	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2YIS9-1 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 4D76D2727A435D2D	FASTA	352	40,083
	10 20 30 40 50 60 MSGTDKVRVN VSQTAQTPLH TSAKLPKVGV LLVNLGTPDG TSYGPMRRYL AEFLSDRRVI 70 80 90 100 110 120 EWSRLIWYPI LYGIVLNTRP RRSGRLYDRI WNHENNESPL RTYTRAQGEK LAKALSDQPN 130 140 150 160 170 180 VVVDWAMRYG QPSIESITDR LLQQGCERIV IFPLYPQYSA TTTATVNDKF FEALMKKRFM 190 200 210 220 230 240 PAIRTVPSYE AEPVYIDALA RSVEKHLATL SFKPEVILTS YHGIPKSYSD KGDPYRQQCL 250 260 270 280 290 300 ETTRLLRERL GLGEDEMRAT FQSRFGPEEW LQPYTDETVK ELAKNGVKLV AVLNPGFVAD 310 320 330 340 350 CLETVDEIGN EAAEEFLENG GENFSHIPCL NDSEEGMKVI ETLVRRELLG WV

References

[1]

"Whole-genome analyses of speciation events in pathogenic Brucellae." Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E. Infect. Immun. 73:8353-8361(2005) [PubMed: 16299333] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM040265 Genomic DNA. Translation: CAJ12241.1.
RefSeq	YP_418320.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2YIS9.
Genome annotation databases
GeneID	3828247.
GenomeReviews	Gene locus BAB2_0075 in contig AM040265_GR.
KEGG	bmf:BAB2_0075.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2YIS9.
OMA	TIEEIGM.
Enzyme and pathway databases
BioCyc	BMEL359391:BAB2_0075-MON.
Family and domain databases
HAMAP	MF_00323. [Tree]
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
ProDom	PD002792. Ferrochelatase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00109. hemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH_BRUA2

Accession

Primary (citable) accession number: Q2YIS9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	December 20, 2005
Last modified:	November 3, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)