Reviewed, UniProtKB/Swiss-Prot Q2YIL5 (HUTI_BRUA2)

Names and origin

Protein names

Recommended name:
    Imidazolonepropionase
    EC=3.5.2.7
Alternative name(s):
    Imidazolone-5-propionate hydrolase

Gene names

Name:	hutI
Ordered Locus Names:	BAB2_0306

Organism

Brucella abortus (strain 2308) [Complete proteome] [HAMAP]

Taxonomic identifier

359391 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	405 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+. HAMAP MF_00372
Cofactor	Binds 1 zinc or iron ion per subunit By similarity.
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3. HAMAP MF_00372
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the hutI family.

Ontologies

Keywords
Biological process	Histidine metabolism
Cellular component	Cytoplasm
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidine catabolic process to glutamate and formamide Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	imidazolonepropionase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 405	405	Imidazolonepropionase HAMAP MF_00372		PRO_0000306444
Sites
	Metal binding	73	1	Zinc or iron By similarity
	Metal binding	75	1	Zinc or iron By similarity
	Metal binding	243	1	Zinc or iron By similarity
	Metal binding	318	1	Zinc or iron By similarity
	Binding site	82	1	Substrate By similarity
	Binding site	95	1	Substrate By similarity
	Binding site	145	1	Substrate By similarity
	Binding site	178	1	Substrate By similarity
	Binding site	246	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2YIL5-1 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: A55470DFB24E994E	FASTA	405	43,520
	10 20 30 40 50 60 MTKNSSTVFT HARIATLEEK AANLGLIEEA ALVVKDARIV YAGPENKLPD EYASFEKIDC 70 80 90 100 110 120 GNRLITPGLI DCHTHLVHAG NRAHEFELRL QGATYEEVAR AGGGIVSSVR NLRAASEDDL 130 140 150 160 170 180 VRETLPRLDA LIAEGVTTVE VKSGYGLDRD SEIKSLKAAR RLGEERDVAI RTTFLGAHAL 190 200 210 220 230 240 PPEMNGDKAA YIDRVINDML PAIAEQGLAD AVDGFCEGIA FLPDEIARVF DAAKAHDIPV 250 260 270 280 290 300 KLHADQLSNL HGAALAASYG ALSADHLEYT DADGAAAMAS AGTVAVLLPG AYYFIRETQK 310 320 330 340 350 360 PPVEAFRAAG TKMALATDNN PGTSPLTSLL LTMNMGATLF RMTVEECIAG VTREAARALG 370 380 390 400 ILDQTGTLEI GKDADLAIWD IERPAELVYR IGFNPLWKRV FKGQI

References

[1]

"Whole-genome analyses of speciation events in pathogenic Brucellae." Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E. Infect. Immun. 73:8353-8361(2005) [PubMed: 16299333] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM040265 Genomic DNA. Translation: CAJ12472.1.
RefSeq	YP_418517.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2YIL5.
Genome annotation databases
GeneID	3827134.
GenomeReviews	Gene locus BAB2_0306 in contig AM040265_GR.
KEGG	bmf:BAB2_0306.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2YIL5.
OMA	MNMACTL.
Enzyme and pathway databases
BioCyc	BMEL359391:BAB2_0306-MON.
Family and domain databases
HAMAP	MF_00372. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR005920. HutI. [Graphical view]
Pfam	PF01979. Amidohydro_1. 2 hits. [Graphical view]
ProDom	PD001248. Amidohydro_like. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01224. hutI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HUTI_BRUA2

Accession

Primary (citable) accession number: Q2YIL5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	February 7, 2006
Last modified:	November 3, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)