Q2YIH4 (LPXK_BRUA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tetraacyldisaccharide 4'-kinase
EC=2.7.1.130

Alternative name(s):
Lipid A 4'-kinase

Gene names

Name:	lpxK
Ordered Locus Names:	BAB2_0210

Organism

Brucella abortus (strain 2308) [Complete proteome] [HAMAP]

Taxonomic identifier

359391 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA) By similarity. HAMAP MF_00409
Catalytic activity	ATP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-4-O-phosphono-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate). HAMAP MF_00409
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 6/6. HAMAP MF_00409
Sequence similarities	Belongs to the LpxK family.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tetraacyldisaccharide 4'-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Tetraacyldisaccharide 4'-kinase HAMAP MF_00409		PRO_0000229946
Regions
	Nucleotide binding	54 – 61	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q2YIH4 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 93014F0F6B7C3DCE	FASTA	341	36,782
	10 20 30 40 50 60 MASEAPPFWW DEPDWRALAL APAAWIYGRV SGRRLIRAVP PRVSLPVLCV GNFTVGGAGK 70 80 90 100 110 120 TPTAIAFARG AIARGMKPGI VSRGYGGNYS GLHLVDPGHD GARHVGDEPL LLARHAAVAL 130 140 150 160 170 180 SPDRVKAAEY LKSLGCDFII MDDGFQSARL HADFSLLVVD ASRGIGNGRV IPAGPLRAPL 190 200 210 220 230 240 TDQMRKTDAL LCIGKGNGAD FVIRQAARAG RPIYHAQLRP SSSATVAGRR WLAFAGIGNP 250 260 270 280 290 300 DKFYESVRQA GGEVVETHSF ADHYSFEPDD IRGLVDMARR QGLGLITTAK DHVRLATMPG 310 320 330 340 VPPEFLSKLA VLDVDLEFDR TDALDHILDT VVERFKSRLH G

References

[1]

"Whole-genome analyses of speciation events in pathogenic Brucellae." Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E. Infect. Immun. 73:8353-8361(2005) [PubMed: 16299333] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 2308.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM040265 Genomic DNA. Translation: CAJ12376.1.
RefSeq	YP_418436.1. NC_007624.1.
3D structure databases
ProteinModelPortal	Q2YIH4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2YIH4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3827177.
GenomeReviews	Gene locus BAB2_0210 in contig AM040265_GR.
KEGG	bmf:BAB2_0210.
PATRIC	17847925. VBIBruMel86222_2495.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1663.
HOGENOM	HBG663914.
OMA	VILMDDG.
ProtClustDB	PRK00652.
Enzyme and pathway databases
BioCyc	BMEL359391:BAB2_0210-MONOMER.
Family and domain databases
HAMAP	MF_00409. LpxK. [Tree]
InterPro	IPR003758. Tetraacyldisaccharide_4-kinase. [Graphical view]
KO	K00912.
Pfam	PF02606. LpxK. 1 hit. [Graphical view]
TIGRFAMs	TIGR00682. LpxK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LPXK_BRUA2

Accession

Primary (citable) accession number: Q2YIH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	February 7, 2006
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program