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Protein

OTU domain-containing protein 5

Gene

Otud5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Deubiquitinating enzyme that functions as negative regulator of the innate immune system. Acts via TRAF3 deubiquitination and subsequent suppression of type I interferon (IFN) production. Has peptidase activity towards 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Can also cleave 'Lys-11'-linked ubiquitin chains (in vitro) (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Enzyme regulationi

Inhibited by N-ethyl-maleimide (NEM).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei221Sequence analysis1
Active sitei224NucleophileBy similarity1
Active sitei329By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC85.001.

Names & Taxonomyi

Protein namesi
Recommended name:
OTU domain-containing protein 5 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme A
Short name:
DUBA
Gene namesi
Name:Otud5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1563027. Otud5.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002782251 – 566OTU domain-containing protein 5Add BLAST566

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei64PhosphoserineCombined sources1
Modified residuei165PhosphoserineCombined sources1
Modified residuei175PhosphotyrosineBy similarity1
Modified residuei177PhosphoserineCombined sources1
Modified residuei447PhosphoserineBy similarity1
Modified residuei502PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylation at Ser-177 is required for deubiquitinating activity.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ2YDU3.
PRIDEiQ2YDU3.

PTM databases

iPTMnetiQ2YDU3.
PhosphoSitePlusiQ2YDU3.

Expressioni

Gene expression databases

BgeeiENSRNOG00000008764.
GenevisibleiQ2YDU3. RN.

Interactioni

Subunit structurei

Interacts with TRAF3.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000039455.

Structurei

3D structure databases

ProteinModelPortaliQ2YDU3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini213 – 336OTUPROSITE-ProRule annotationAdd BLAST124

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni218 – 224Cys-loopBy similarity7
Regioni273 – 283Variable-loopBy similarityAdd BLAST11
Regioni324 – 329His-loopBy similarity6

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi5 – 113Pro-richAdd BLAST109
Compositional biasi33 – 174Gly-richAdd BLAST142

Sequence similaritiesi

Belongs to the peptidase C85 family.Curated
Contains 1 OTU domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2605. Eukaryota.
ENOG410XNYW. LUCA.
HOGENOMiHOG000231360.
HOVERGENiHBG060214.
InParanoidiQ2YDU3.
KOiK12655.
OrthoDBiEOG091G0NE6.
PhylomeDBiQ2YDU3.

Family and domain databases

InterProiIPR031084. OTU5/OTLD1.
IPR003323. OTU_dom.
[Graphical view]
PANTHERiPTHR12419:SF4. PTHR12419:SF4. 1 hit.
PfamiPF02338. OTU. 1 hit.
[Graphical view]
PROSITEiPS50802. OTU. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q2YDU3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTILPKKKPP PPDADPANEP PPPGPLPPAP RRGGGVGVGG GGTGVGGGER
60 70 80 90 100
DRDSGVVGAR PRASPPPQGP LPGPPGALHR WALAVPPGAV AGPRPQQASP
110 120 130 140 150
PPCGGPGGPG GGPGDALGAT TAGVGAAGVV VGVGGPVGVG GCCSGPGHSK
160 170 180 190 200
RRRQAPGVGA VGGASPEREE VGAGYNSEDE YEAAAARIEA MDPATVEQQE
210 220 230 240 250
HWFEKALRDK KGFIIKQMKE DGACLFRAVA DQVYGDQDMH EVVRKHCMDY
260 270 280 290 300
LMKNADYFSN YVTEDFTTYI NRKRKNNCHG NHIEMQAMAE MYNRPVEVYQ
310 320 330 340 350
YSTEPINTFH GIHQNEDEPI RVSYHRNIHY NSVVNPNKAT IGVGLGLPSF
360 370 380 390 400
KPGFAEQSLM KNAIKTSEES WIEQQMLEDK KRATDWEATN EAIEEQVARE
410 420 430 440 450
SYLQWLRDQE KQARQVRGPS QPRKASATCS SATAAASSGL EEWTSRSPRQ
460 470 480 490 500
RSSASSPEHP ELHAELGIKP PSPGTVLALA KPPSPCAPGT SSQFSAGADR
510 520 530 540 550
ATSPLVSLYP ALECRALIQQ MSPSAFGLND WDDDEILASV LAVSQQEYLD
560
SMKKNKVHRD PPPDKS
Length:566
Mass (Da):60,288
Last modified:February 20, 2007 - v2
Checksum:i1B49AF4337634A07
GO
Isoform 2 (identifier: Q2YDU3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     118-160: Missing.

Show »
Length:523
Mass (Da):56,534
Checksum:iCB83BE543DEACE23
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_023197118 – 160Missing in isoform 2. 1 PublicationAdd BLAST43

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03119131 Genomic DNA. No translation available.
BC110054 mRNA. Translation: AAI10055.1.
RefSeqiNP_001032585.1. NM_001037496.1. [Q2YDU3-2]
UniGeneiRn.97794.

Genome annotation databases

GeneIDi363452.
KEGGirno:363452.
UCSCiRGD:1563027. rat. [Q2YDU3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03119131 Genomic DNA. No translation available.
BC110054 mRNA. Translation: AAI10055.1.
RefSeqiNP_001032585.1. NM_001037496.1. [Q2YDU3-2]
UniGeneiRn.97794.

3D structure databases

ProteinModelPortaliQ2YDU3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000039455.

Protein family/group databases

MEROPSiC85.001.

PTM databases

iPTMnetiQ2YDU3.
PhosphoSitePlusiQ2YDU3.

Proteomic databases

PaxDbiQ2YDU3.
PRIDEiQ2YDU3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi363452.
KEGGirno:363452.
UCSCiRGD:1563027. rat. [Q2YDU3-1]

Organism-specific databases

CTDi55593.
RGDi1563027. Otud5.

Phylogenomic databases

eggNOGiKOG2605. Eukaryota.
ENOG410XNYW. LUCA.
HOGENOMiHOG000231360.
HOVERGENiHBG060214.
InParanoidiQ2YDU3.
KOiK12655.
OrthoDBiEOG091G0NE6.
PhylomeDBiQ2YDU3.

Miscellaneous databases

PROiQ2YDU3.

Gene expression databases

BgeeiENSRNOG00000008764.
GenevisibleiQ2YDU3. RN.

Family and domain databases

InterProiIPR031084. OTU5/OTLD1.
IPR003323. OTU_dom.
[Graphical view]
PANTHERiPTHR12419:SF4. PTHR12419:SF4. 1 hit.
PfamiPF02338. OTU. 1 hit.
[Graphical view]
PROSITEiPS50802. OTU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOTUD5_RAT
AccessioniPrimary (citable) accession number: Q2YDU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: February 20, 2007
Last modified: November 2, 2016
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.