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Q2YDF1

- TRMB_BOVIN

UniProt

Q2YDF1 - TRMB_BOVIN

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

METTL1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (20 Dec 2005)
      Previous versions | rss
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    • Comment

    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei85 – 851S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Binding sitei161 – 1611S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Active sitei164 – 1641UniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB
    2. tRNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. tRNA modification Source: UniProtKB

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    RNA-binding, S-adenosyl-L-methionine, tRNA-binding

    Enzyme and pathway databases

    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
    Alternative name(s):
    Methyltransferase-like protein 1UniRule annotation
    tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
    tRNA(m7G46)-methyltransferaseUniRule annotation
    Gene namesi
    Name:METTL1UniRule annotation
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 5

    Subcellular locationi

    Nucleus UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: Ensembl
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 277277tRNA (guanine-N(7)-)-methyltransferasePRO_0000284065Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei28 – 281Phosphoserine; by PKB and RPS6KA3UniRule annotation

    Post-translational modificationi

    Phosphorylation at Ser-28 inactivates its catalytic activity but does not affect the interaction with WDR4.UniRule annotation

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ2YDF1.

    Interactioni

    Subunit structurei

    Forms a complex with WDR4.UniRule annotation

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000022487.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2YDF1.
    SMRiQ2YDF1. Positions 38-265.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni108 – 1092S-adenosyl-L-methionine bindingUniRule annotation
    Regioni141 – 1422S-adenosyl-L-methionine bindingUniRule annotation
    Regioni239 – 2413S-adenosyl-L-methionine bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0220.
    GeneTreeiENSGT00390000017840.
    HOGENOMiHOG000260965.
    HOVERGENiHBG044569.
    InParanoidiQ2YDF1.
    KOiK03439.
    OMAiRAHSNPI.
    OrthoDBiEOG7PS1GC.
    TreeFamiTF314083.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q2YDF1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGTETGDAA GTEAPQPQKR YYRQRAHSNP MADHTLRYPV KPEDMDWSEL    50
    YPEFFAPLPQ NQSHDDPKDK KEKRAQAQVE FADIGCGYGG LLVELSPLFP 100
    DTLILGLEIR VKVSDYVQDR IRALRAAPGG GFQNIACLRS NAMKHLPNFF 150
    HKGQLTKMFF LFPDPHFKRT KHKWRIISPT LLAEYAYVLR VGGLVYTITD 200
    VLELHEWMCT HFEGHPLFER VPLEELSEDP IVGHLGTSTE EGKKVLRNGG 250
    KNFPAIFRRI QDPALPAVTP TPTPPGH 277
    Length:277
    Mass (Da):31,323
    Last modified:December 20, 2005 - v1
    Checksum:i4860363A97727ADC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BT029912 mRNA. Translation: ABM06158.1.
    BC110252 mRNA. Translation: AAI10253.1.
    RefSeqiNP_001039542.1. NM_001046077.2.
    UniGeneiBt.5722.

    Genome annotation databases

    EnsembliENSBTAT00000022487; ENSBTAP00000022487; ENSBTAG00000016908.
    GeneIDi511197.
    KEGGibta:511197.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BT029912 mRNA. Translation: ABM06158.1 .
    BC110252 mRNA. Translation: AAI10253.1 .
    RefSeqi NP_001039542.1. NM_001046077.2.
    UniGenei Bt.5722.

    3D structure databases

    ProteinModelPortali Q2YDF1.
    SMRi Q2YDF1. Positions 38-265.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000022487.

    Proteomic databases

    PRIDEi Q2YDF1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000022487 ; ENSBTAP00000022487 ; ENSBTAG00000016908 .
    GeneIDi 511197.
    KEGGi bta:511197.

    Organism-specific databases

    CTDi 4234.

    Phylogenomic databases

    eggNOGi COG0220.
    GeneTreei ENSGT00390000017840.
    HOGENOMi HOG000260965.
    HOVERGENi HBG044569.
    InParanoidi Q2YDF1.
    KOi K03439.
    OMAi RAHSNPI.
    OrthoDBi EOG7PS1GC.
    TreeFami TF314083.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .

    Miscellaneous databases

    NextBioi 20869815.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_03055. tRNA_methyltr_TrmB_euk.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Crossbred X Angus.
      Tissue: Liver.

    Entry informationi

    Entry nameiTRMB_BOVIN
    AccessioniPrimary (citable) accession number: Q2YDF1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 17, 2007
    Last sequence update: December 20, 2005
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3