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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

METTL1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei85S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei161S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei164UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
Methyltransferase-like protein 1UniRule annotation
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:METTL1UniRule annotation
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 5

Organism-specific databases

VGNCiVGNC:31400 METTL1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002840651 – 277tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST277

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei28Phosphoserine; by PKB and RPS6KA3UniRule annotationBy similarity1

Post-translational modificationi

Phosphorylation at Ser-28 inactivates its catalytic activity but does not affect the interaction with WDR4.UniRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ2YDF1
PRIDEiQ2YDF1

Expressioni

Gene expression databases

BgeeiENSBTAG00000016908

Interactioni

Subunit structurei

Forms a complex with WDR4.UniRule annotation

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000022487

Structurei

3D structure databases

ProteinModelPortaliQ2YDF1
SMRiQ2YDF1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni108 – 109S-adenosyl-L-methionine bindingUniRule annotation2
Regioni141 – 142S-adenosyl-L-methionine bindingUniRule annotation2
Regioni239 – 241S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiKOG3115 Eukaryota
COG0220 LUCA
GeneTreeiENSGT00390000017840
HOGENOMiHOG000260965
HOVERGENiHBG044569
InParanoidiQ2YDF1
KOiK03439
OMAiIGMEIRV
OrthoDBiEOG091G0IQ7
TreeFamiTF314083

Family and domain databases

HAMAPiMF_03055 tRNA_methyltr_TrmB_euk, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR025763 Trm8_euk
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
PTHR23417:SF16 PTHR23417:SF16, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

Q2YDF1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGTETGDAA GTEAPQPQKR YYRQRAHSNP MADHTLRYPV KPEDMDWSEL
60 70 80 90 100
YPEFFAPLPQ NQSHDDPKDK KEKRAQAQVE FADIGCGYGG LLVELSPLFP
110 120 130 140 150
DTLILGLEIR VKVSDYVQDR IRALRAAPGG GFQNIACLRS NAMKHLPNFF
160 170 180 190 200
HKGQLTKMFF LFPDPHFKRT KHKWRIISPT LLAEYAYVLR VGGLVYTITD
210 220 230 240 250
VLELHEWMCT HFEGHPLFER VPLEELSEDP IVGHLGTSTE EGKKVLRNGG
260 270
KNFPAIFRRI QDPALPAVTP TPTPPGH
Length:277
Mass (Da):31,323
Last modified:December 20, 2005 - v1
Checksum:i4860363A97727ADC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT029912 mRNA Translation: ABM06158.1
BC110252 mRNA Translation: AAI10253.1
RefSeqiNP_001039542.1, NM_001046077.2
UniGeneiBt.5722

Genome annotation databases

EnsembliENSBTAT00000022487; ENSBTAP00000022487; ENSBTAG00000016908
GeneIDi511197
KEGGibta:511197

Similar proteinsi

Entry informationi

Entry nameiTRMB_BOVIN
AccessioniPrimary (citable) accession number: Q2YDF1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: December 20, 2005
Last modified: March 28, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health