Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q2YDF1 (TRMB_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
Methyltransferase-like protein 1
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:METTL1
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length277 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_03055

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_03055

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_03055

Subunit structure

Forms a complex with WDR4 By similarity. HAMAP-Rule MF_03055

Subcellular location

Nucleus By similarity HAMAP-Rule MF_03055.

Post-translational modification

Phosphorylation at Ser-28 inactivates its catalytic activity but does not affect the interaction with WDR4 By similarity. HAMAP-Rule MF_03055

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 277277tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_03055
PRO_0000284065

Regions

Region108 – 1092S-adenosyl-L-methionine binding By similarity
Region141 – 1422S-adenosyl-L-methionine binding By similarity
Region239 – 2413S-adenosyl-L-methionine binding By similarity

Sites

Active site1641 By similarity
Binding site851S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site1611S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Amino acid modifications

Modified residue281Phosphoserine; by PKB and RPS6KA3 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2YDF1 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: 4860363A97727ADC

FASTA27731,323
        10         20         30         40         50         60 
MAGTETGDAA GTEAPQPQKR YYRQRAHSNP MADHTLRYPV KPEDMDWSEL YPEFFAPLPQ 

        70         80         90        100        110        120 
NQSHDDPKDK KEKRAQAQVE FADIGCGYGG LLVELSPLFP DTLILGLEIR VKVSDYVQDR 

       130        140        150        160        170        180 
IRALRAAPGG GFQNIACLRS NAMKHLPNFF HKGQLTKMFF LFPDPHFKRT KHKWRIISPT 

       190        200        210        220        230        240 
LLAEYAYVLR VGGLVYTITD VLELHEWMCT HFEGHPLFER VPLEELSEDP IVGHLGTSTE 

       250        260        270 
EGKKVLRNGG KNFPAIFRRI QDPALPAVTP TPTPPGH 

« Hide

References

[1]"Characterization of 954 bovine full-CDS cDNA sequences."
Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.
BMC Genomics 6:166-166(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]NIH - Mammalian Gene Collection (MGC) project
Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Crossbred X Angus.
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BT029912 mRNA. Translation: ABM06158.1.
BC110252 mRNA. Translation: AAI10253.1.
RefSeqNP_001039542.1. NM_001046077.2.
UniGeneBt.5722.

3D structure databases

ProteinModelPortalQ2YDF1.
SMRQ2YDF1. Positions 38-265.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000022487.

Proteomic databases

PRIDEQ2YDF1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000022487; ENSBTAP00000022487; ENSBTAG00000016908.
GeneID511197.
KEGGbta:511197.

Organism-specific databases

CTD4234.

Phylogenomic databases

eggNOGCOG0220.
GeneTreeENSGT00390000017840.
HOGENOMHOG000260965.
HOVERGENHBG044569.
InParanoidQ2YDF1.
KOK03439.
OMARAHSNPI.
OrthoDBEOG7PS1GC.
TreeFamTF314083.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_03055. tRNA_methyltr_TrmB_euk.
InterProIPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20869815.

Entry information

Entry nameTRMB_BOVIN
AccessionPrimary (citable) accession number: Q2YDF1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: December 20, 2005
Last modified: June 11, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways