Reviewed, UniProtKB/Swiss-Prot Q2YDE4 (PSA6_BOVIN)

Names and origin

Protein names

Recommended name:
    Proteasome subunit alpha type-6
    EC=3.4.25.1

Gene names

Name:

PSMA6

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity By similarity.
Catalytic activity	Cleavage of peptide bonds with very broad specificity.
Subunit structure	The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the peptidase T1A family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus Proteasome
Molecular function	Hydrolase Protease Threonine protease
PTM	Acetylation Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	3D-structure
Gene Ontology (GO)
Biological process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell proteasome core complex, alpha-subunit complex Inferred from electronic annotation. Source: InterPro
Molecular function	threonine-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 246

246

Proteasome subunit alpha type-6

PRO_0000274034

Amino acid modifications

Modified residue

102

1

N6-acetyllysine By similarity

Modified residue

104

1

N6-acetyllysine By similarity

Modified residue

159

1

Phosphotyrosine By similarity

Modified residue

160

1

Phosphotyrosine By similarity

Cross-link

59

Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Secondary structure

1

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246

Helix Strand Turn

Details...

Turn

10 – 12

3

Helix

23 – 33

11

Beta strand

39 – 42

4

Beta strand

47 – 53

7

Beta strand

59 – 61

3

Helix

63 – 65

3

Beta strand

72 – 74

3

Beta strand

79 – 81

3

Helix

84 – 104

21

Helix

111 – 127

17

Beta strand

129 – 131

3

Beta strand

137 – 140

4

Beta strand

152 – 154

3

Beta strand

165 – 168

4

Helix

172 – 182

11

Helix

191 – 205

15

Beta strand

214 – 216

3

Helix

232 – 243

12

Sequences

	Sequence		Length	Mass (Da)
	Q2YDE4-1 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 94D1FD3C0A7CC72A	FASTA	246	27,399
	10 20 30 40 50 60 MSRGSSAGFD RHITIFSPEG RLYQVEYAFK AINQGGLTSV AVRGKDCAVI VTQKKVPDKL 70 80 90 100 110 120 LDSSTVTHLF KITENIGCVM TGMTADSRSQ VQRARYEAAN WKYKYGYEIP VDMLCKRIAD 130 140 150 160 170 180 ISQVYTQNAE MRPLGCCMIL IGIDEEQGPQ VYKCDPAGYY CGFKATAAGV KQTESTSFLE 190 200 210 220 230 240 KKVKKKFDWT FEQTVETAIT CLSTVLSIDF KPSEIEVGVV TVENPKFRIL TEAEIDAHLV ALAERD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver.
[2]	"The structure of the mammalian 20S proteasome at 2.75 A resolution." Unno M., Mizushima T., Morimoto Y., Tomisugi Y., Tanaka K., Yasuoka N., Tsukihara T. Structure 10:609-618(2002) [PubMed: 12015144] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.75 ANGSTROMS) OF COMPLEX WITH THE 20S PROTEASOME.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BC110260 mRNA. Translation: AAI10261.1.

IPI

IPI00715748.

RefSeq

NP_001039427.1.

UniGene

Bt.15837

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1IRU	X-ray	2.75	A/O	1-246	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

Q2YDE4.

Protein family/group databases

MEROPS

T01.971.

Proteomic databases

PRIDE

Q2YDE4.

Genome annotation databases

Ensembl

ENSBTAT00000012773; ENSBTAP00000012773; ENSBTAG00000009683; Bos taurus. [Genome view]

GeneID

507213.

KEGG

bta:507213.

Organism-specific databases

CTD

507213.

Phylogenomic databases

eggNOG

maNOG11448.

HOVERGEN

Q2YDE4.

InParanoid

Q2YDE4.

OMA

RYKYGYE.

OrthoDB

EOG9R7XW1.

PhylomeDB

Q2YDE4.

Enzyme and pathway databases

BRENDA

3.4.25.1. 251.

Family and domain databases

InterPro

IPR000426. Proteasome_asu_CS.
IPR001353. Proteasome_sua/b.
[Graphical view]

Pfam

PF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view]

PROSITE

PS00388. PROTEASOME_A_1. 1 hit.
PS51475. PROTEASOME_A_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

PSA6_BOVIN

Accession

Primary (citable) accession number: Q2YDE4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	December 20, 2005
Last modified:	February 9, 2010
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)