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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Nitrosospira multiformis (strain ATCC 25196 / NCIMB 11849 / C 71)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei59S-adenosyl-L-methionineUniRule annotation1
Binding sitei84S-adenosyl-L-methionineUniRule annotation1
Binding sitei111S-adenosyl-L-methionineUniRule annotation1
Active sitei134By similarity1
Binding sitei134S-adenosyl-L-methionineUniRule annotation1
Binding sitei138SubstrateUniRule annotation1
Binding sitei170SubstrateUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciNMUL323848:G1GTI-71-MONOMER
UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Nmul_A0069
OrganismiNitrosospira multiformis (strain ATCC 25196 / NCIMB 11849 / C 71)
Taxonomic identifieri323848 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosospira
Proteomesi
  • UP000002718 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002291801 – 229tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST229

Interactioni

Protein-protein interaction databases

STRINGi323848.Nmul_A0069

Structurei

3D structure databases

ProteinModelPortaliQ2YCZ3
SMRiQ2YCZ3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni205 – 208Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1 Bacteria
COG0220 LUCA
HOGENOMiHOG000073968
KOiK03439
OMAiPDPWHKS
OrthoDBiPOG091H015P

Family and domain databases

HAMAPiMF_01057 tRNA_methyltr_TrmB, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

Q2YCZ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVEHSPHRPI RSFVLRQGRL SNAQRRAHET LMPKYGIPYS GKLLDLAIIF
60 70 80 90 100
SRSAPKFLEI GFGMGETTAL IAQAHPQNDY LAAEVHTPGM GSLLKQVEEL
110 120 130 140 150
ELTNIRVIQH DAVDVLQHAL PPECLDGVHV FFPDPWPKAR HHKRRLIQAE
160 170 180 190 200
FVDLLCSRLK PGGYIHVATD WEDYAEQILE VLSGEPHLSN TAVGYAPRPE
210 220
YRPLTKFEQR GLRLGHEVWD VIFRKKQGA
Length:229
Mass (Da):26,016
Last modified:December 20, 2005 - v1
Checksum:i55094E6B1C45D357
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000103 Genomic DNA Translation: ABB73378.1
RefSeqiWP_011379433.1, NZ_FNVK01000041.1

Genome annotation databases

EnsemblBacteriaiABB73378; ABB73378; Nmul_A0069
KEGGinmu:Nmul_A0069

Similar proteinsi

Entry informationi

Entry nameiTRMB_NITMU
AccessioniPrimary (citable) accession number: Q2YCZ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: December 20, 2005
Last modified: May 23, 2018
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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