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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Nitrosospira multiformis (strain ATCC 25196 / NCIMB 11849 / C 71)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei627ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Nmul_A0511
OrganismiNitrosospira multiformis (strain ATCC 25196 / NCIMB 11849 / C 71)
Taxonomic identifieri323848 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosospira
Proteomesi
  • UP000002718 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000093801 – 866Leucine--tRNA ligaseAdd BLAST866

Interactioni

Protein-protein interaction databases

STRINGi323848.Nmul_A0511.

Structurei

3D structure databases

ProteinModelPortaliQ2YBQ2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi42 – 52"HIGH" regionAdd BLAST11
Motifi624 – 628"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C8T. Bacteria.
COG0495. LUCA.
HOGENOMiHOG000200747.
KOiK01869.
OMAiHRFIKRL.
OrthoDBiPOG091H01JZ.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 2 hits.
HAMAPiMF_00049_B. Leu_tRNA_synth_B. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 3 hits.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2YBQ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQEKYHPQEI ESEAQQYWQQ TAAFKAVEAP EKRKYYCLSM FPYPSGKLHM
60 70 80 90 100
GHVRNYTIGD VLSRYHRMQG YNVLQPMGWD AFGLPAENAA MQNNVPPAKW
110 120 130 140 150
TYDNIAYMRK QLQSLGLAMD WDRELATCQP DYYRWNQWLF LRMLEKGLAY
160 170 180 190 200
RTTGIVNWDP VDQTVLANEQ VIDGRGWRTG ALVEKHEIPM YYMKITAYAD
210 220 230 240 250
ELLEALNALP GWPERVRTMQ ANWIGKSFGV EVRFPADAES GMPQDLKVFT
260 270 280 290 300
TRADTLFGVT YVAVAAEHPV AQHAAKSNPA LAAFIEECRQ GAMMEAELAT
310 320 330 340 350
QEKKGRDTGL YVIHPLTGAR LPVWIANYVL MGYGEGAVMA VPAHDERDFE
360 370 380 390 400
FATQYSLPIR AVIKPVDSGL TVPLAQAYVE HGITFDSGEF SGLAFQPAVD
410 420 430 440 450
AIAVALQQKG LGEKRVHYRL RDWGISRQRY WGCPIPLIYC DACGVVPVPD
460 470 480 490 500
EQLPVVLPED LVPDGSGNPL AKTPSFYECS CPRCGQSARR ETDTMDTFVD
510 520 530 540 550
SSWYYIRYAC TDQHRAMVDA RVDYWLPVDQ YIGGIEHAIL HLLYSRFWSK
560 570 580 590 600
VMRDLGLVLF DEPFANLLTQ GMVLNEIMFR KTGSGRIVYF NPADVDIQTD
610 620 630 640 650
EQGRRIGAVL RADGQPVEAG GIGTMSKSRN NGVDPQKLVE QYGADTARLF
660 670 680 690 700
MMFASPPEQT LEWADAGVEG AFRFLKRLWK QVYDHLQQSG VANGPIPVAG
710 720 730 740 750
LGPELKALRF QLHQTIAKVG DDLGRRHTFN TAIAAVMELM NALGKLQDSS
760 770 780 790 800
PSARGLMQEA LENIVLLLSP IVPHICHVLW RELRPGTELL DQPWPQADVA
810 820 830 840 850
ALVQDEIELI VQVNGKLRGK IRVAADTKPV IVEQLALENE QVRRFIDGKA
860
VKKVVMVPGK LVNIVI
Length:866
Mass (Da):97,239
Last modified:December 20, 2005 - v1
Checksum:i9D6E9F9C850663CF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000103 Genomic DNA. Translation: ABB73819.1.
RefSeqiWP_011379873.1. NC_007614.1.

Genome annotation databases

EnsemblBacteriaiABB73819; ABB73819; Nmul_A0511.
KEGGinmu:Nmul_A0511.
PATRICi22724022. VBINitMul110821_0587.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000103 Genomic DNA. Translation: ABB73819.1.
RefSeqiWP_011379873.1. NC_007614.1.

3D structure databases

ProteinModelPortaliQ2YBQ2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi323848.Nmul_A0511.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABB73819; ABB73819; Nmul_A0511.
KEGGinmu:Nmul_A0511.
PATRICi22724022. VBINitMul110821_0587.

Phylogenomic databases

eggNOGiENOG4105C8T. Bacteria.
COG0495. LUCA.
HOGENOMiHOG000200747.
KOiK01869.
OMAiHRFIKRL.
OrthoDBiPOG091H01JZ.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 2 hits.
HAMAPiMF_00049_B. Leu_tRNA_synth_B. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 3 hits.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYL_NITMU
AccessioniPrimary (citable) accession number: Q2YBQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.