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Q2YBQ2 (SYL_NITMU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Nmul_A0511
OrganismNitrosospira multiformis (strain ATCC 25196 / NCIMB 11849) [Complete proteome] [HAMAP]
Taxonomic identifier323848 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosospira

Protein attributes

Sequence length866 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 866866Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009380

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif624 – 6285"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6271ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2YBQ2 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: 9D6E9F9C850663CF

FASTA86697,239
        10         20         30         40         50         60 
MQEKYHPQEI ESEAQQYWQQ TAAFKAVEAP EKRKYYCLSM FPYPSGKLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VLSRYHRMQG YNVLQPMGWD AFGLPAENAA MQNNVPPAKW TYDNIAYMRK QLQSLGLAMD 

       130        140        150        160        170        180 
WDRELATCQP DYYRWNQWLF LRMLEKGLAY RTTGIVNWDP VDQTVLANEQ VIDGRGWRTG 

       190        200        210        220        230        240 
ALVEKHEIPM YYMKITAYAD ELLEALNALP GWPERVRTMQ ANWIGKSFGV EVRFPADAES 

       250        260        270        280        290        300 
GMPQDLKVFT TRADTLFGVT YVAVAAEHPV AQHAAKSNPA LAAFIEECRQ GAMMEAELAT 

       310        320        330        340        350        360 
QEKKGRDTGL YVIHPLTGAR LPVWIANYVL MGYGEGAVMA VPAHDERDFE FATQYSLPIR 

       370        380        390        400        410        420 
AVIKPVDSGL TVPLAQAYVE HGITFDSGEF SGLAFQPAVD AIAVALQQKG LGEKRVHYRL 

       430        440        450        460        470        480 
RDWGISRQRY WGCPIPLIYC DACGVVPVPD EQLPVVLPED LVPDGSGNPL AKTPSFYECS 

       490        500        510        520        530        540 
CPRCGQSARR ETDTMDTFVD SSWYYIRYAC TDQHRAMVDA RVDYWLPVDQ YIGGIEHAIL 

       550        560        570        580        590        600 
HLLYSRFWSK VMRDLGLVLF DEPFANLLTQ GMVLNEIMFR KTGSGRIVYF NPADVDIQTD 

       610        620        630        640        650        660 
EQGRRIGAVL RADGQPVEAG GIGTMSKSRN NGVDPQKLVE QYGADTARLF MMFASPPEQT 

       670        680        690        700        710        720 
LEWADAGVEG AFRFLKRLWK QVYDHLQQSG VANGPIPVAG LGPELKALRF QLHQTIAKVG 

       730        740        750        760        770        780 
DDLGRRHTFN TAIAAVMELM NALGKLQDSS PSARGLMQEA LENIVLLLSP IVPHICHVLW 

       790        800        810        820        830        840 
RELRPGTELL DQPWPQADVA ALVQDEIELI VQVNGKLRGK IRVAADTKPV IVEQLALENE 

       850        860 
QVRRFIDGKA VKKVVMVPGK LVNIVI 

« Hide

References

[1]"Complete sequence of chromosome 1 of Nitrosospira multiformis ATCC 25196."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Richardson P.
Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25196 / NCIMB 11849.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000103 Genomic DNA. Translation: ABB73819.1.
RefSeqYP_411211.1. NC_007614.1.

3D structure databases

ProteinModelPortalQ2YBQ2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING323848.Nmul_A0511.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABB73819; ABB73819; Nmul_A0511.
GeneID3785640.
KEGGnmu:Nmul_A0511.
PATRIC22724022. VBINitMul110821_0587.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycNMUL323848:GKEC-527-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 2 hits.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_NITMU
AccessionPrimary (citable) accession number: Q2YBQ2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: December 20, 2005
Last modified: May 14, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries