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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Nitrosospira multiformis (strain ATCC 25196 / NCIMB 11849)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei418 – 4181NucleophileUniRule annotation
Active sitei471 – 4711Proton donorUniRule annotation

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciNMUL323848:GKEC-734-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Ordered Locus Names:Nmul_A0717
OrganismiNitrosospira multiformis (strain ATCC 25196 / NCIMB 11849)
Taxonomic identifieri323848 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosospira
ProteomesiUP000002718: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7467461,4-alpha-glucan branching enzyme GlgBPRO_0000260672Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi323848.Nmul_A0717.

Structurei

3D structure databases

ProteinModelPortaliQ2YB47.
SMRiQ2YB47. Positions 129-736.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiEGHLYKY.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2YB47-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRAYPTIQRP SIKRDPRLHA LLAGRLHDPF SFLGPHVEHG HWVVRAFHPH
60 70 80 90 100
AASIWINTDD GFESMTRAHP AGVFEWWGLI PLPLPYQLRI EEASFEAPAH
110 120 130 140 150
VYDIYDAYAF PPQVSEHDLY LFNEGRLHQA YRMLGSHLVE MEGVAGVRFS
160 170 180 190 200
VWAPNAERVS VVGSFNRWDG RIHPMVSHGA SGVWELFIPG VPTDSFYKYE
210 220 230 240 250
IRNRDTGAIL LKTDPYARHY EVRPGTAARA PSASRMTWGD ARWMAQRAVW
260 270 280 290 300
DWLATPLNIY EVHAGSWKRH ANGSFYSYRE LADHLLPYVR EMGYTHIELL
310 320 330 340 350
PISEHPLDES WGYQTTGYFA ATSRYGTSDD LKFFINACHQ EGIGVILDWV
360 370 380 390 400
PAHFPQDAFA LARFDGSALY EHEDPRMGLH QDWGTHIFNY GRNEVKSFLL
410 420 430 440 450
SSAHYWLSEF HLDGLRVDAV ASMLYLDYSR LPGEWLPNRY GGRENLEAID
460 470 480 490 500
FLRELNIMVH QEFPGALTLA EESTAWPGVS RPTYVGGLGF SMKWNMGWMN
510 520 530 540 550
DTLVYMRNDP VYRRFHHEQL TFGQLYAYSE NFVLPFSHDE VVHGKGSLLG
560 570 580 590 600
KMPGDAWQQF ANLRLLFTYQ MTYPGKKLNF MGNEFAQGRE WNSSRELDWY
610 620 630 640 650
QLGDGWHEGV KMAARDLNQL YSAVAALHQR DFEPEGFSWV DCHDADNSVI
660 670 680 690 700
SYLRFARNGS FALVVLNFTP VPRYRYRIGV PTSGSYQEIF NSDSRYYGGS
710 720 730 740
DLGNMGSIRT TGQPSMERMD SIVITLPPLA GLIFVHTDLP AINTDG
Length:746
Mass (Da):85,379
Last modified:December 20, 2005 - v1
Checksum:i273B277BCFA45F89
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000103 Genomic DNA. Translation: ABB74024.1.
RefSeqiYP_411416.1. NC_007614.1.

Genome annotation databases

EnsemblBacteriaiABB74024; ABB74024; Nmul_A0717.
GeneIDi3786063.
KEGGinmu:Nmul_A0717.
PATRICi22724500. VBINitMul110821_0825.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000103 Genomic DNA. Translation: ABB74024.1.
RefSeqiYP_411416.1. NC_007614.1.

3D structure databases

ProteinModelPortaliQ2YB47.
SMRiQ2YB47. Positions 129-736.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi323848.Nmul_A0717.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABB74024; ABB74024; Nmul_A0717.
GeneIDi3786063.
KEGGinmu:Nmul_A0717.
PATRICi22724500. VBINitMul110821_0825.

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiEGHLYKY.
OrthoDBiEOG6JX7GT.

Enzyme and pathway databases

UniPathwayiUPA00164.
BioCyciNMUL323848:GKEC-734-MONOMER.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome 1 of Nitrosospira multiformis ATCC 25196."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Richardson P.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25196 / NCIMB 11849.

Entry informationi

Entry nameiGLGB_NITMU
AccessioniPrimary (citable) accession number: Q2YB47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: December 20, 2005
Last modified: February 4, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.