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Q2YB47 (GLGB_NITMU) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:Nmul_A0717
OrganismNitrosospira multiformis (strain ATCC 25196 / NCIMB 11849) [Complete proteome] [HAMAP]
Taxonomic identifier323848 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosospira

Protein attributes

Sequence length746 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7467461,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000260672

Sites

Active site4181Nucleophile By similarity
Active site4711Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2YB47 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: 273B277BCFA45F89

FASTA74685,379
        10         20         30         40         50         60 
MRAYPTIQRP SIKRDPRLHA LLAGRLHDPF SFLGPHVEHG HWVVRAFHPH AASIWINTDD 

        70         80         90        100        110        120 
GFESMTRAHP AGVFEWWGLI PLPLPYQLRI EEASFEAPAH VYDIYDAYAF PPQVSEHDLY 

       130        140        150        160        170        180 
LFNEGRLHQA YRMLGSHLVE MEGVAGVRFS VWAPNAERVS VVGSFNRWDG RIHPMVSHGA 

       190        200        210        220        230        240 
SGVWELFIPG VPTDSFYKYE IRNRDTGAIL LKTDPYARHY EVRPGTAARA PSASRMTWGD 

       250        260        270        280        290        300 
ARWMAQRAVW DWLATPLNIY EVHAGSWKRH ANGSFYSYRE LADHLLPYVR EMGYTHIELL 

       310        320        330        340        350        360 
PISEHPLDES WGYQTTGYFA ATSRYGTSDD LKFFINACHQ EGIGVILDWV PAHFPQDAFA 

       370        380        390        400        410        420 
LARFDGSALY EHEDPRMGLH QDWGTHIFNY GRNEVKSFLL SSAHYWLSEF HLDGLRVDAV 

       430        440        450        460        470        480 
ASMLYLDYSR LPGEWLPNRY GGRENLEAID FLRELNIMVH QEFPGALTLA EESTAWPGVS 

       490        500        510        520        530        540 
RPTYVGGLGF SMKWNMGWMN DTLVYMRNDP VYRRFHHEQL TFGQLYAYSE NFVLPFSHDE 

       550        560        570        580        590        600 
VVHGKGSLLG KMPGDAWQQF ANLRLLFTYQ MTYPGKKLNF MGNEFAQGRE WNSSRELDWY 

       610        620        630        640        650        660 
QLGDGWHEGV KMAARDLNQL YSAVAALHQR DFEPEGFSWV DCHDADNSVI SYLRFARNGS 

       670        680        690        700        710        720 
FALVVLNFTP VPRYRYRIGV PTSGSYQEIF NSDSRYYGGS DLGNMGSIRT TGQPSMERMD 

       730        740 
SIVITLPPLA GLIFVHTDLP AINTDG 

« Hide

References

[1]"Complete sequence of chromosome 1 of Nitrosospira multiformis ATCC 25196."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Richardson P.
Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25196 / NCIMB 11849.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000103 Genomic DNA. Translation: ABB74024.1.
RefSeqYP_411416.1. NC_007614.1.

3D structure databases

ProteinModelPortalQ2YB47.
SMRQ2YB47. Positions 129-736.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING323848.Nmul_A0717.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABB74024; ABB74024; Nmul_A0717.
GeneID3786063.
KEGGnmu:Nmul_A0717.
PATRIC22724500. VBINitMul110821_0825.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAGELSHAW.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycNMUL323848:GKEC-734-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_NITMU
AccessionPrimary (citable) accession number: Q2YB47
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: December 20, 2005
Last modified: June 11, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries