Q2YAZ9 (EFTU_NITMU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor Tu
Short name=EF-Tu

Gene names

Name:	tuf1
Ordered Locus Names:	Nmul_A0752
AND
Name:	tuf2
Ordered Locus Names:	Nmul_A0765

Organism

Nitrosospira multiformis (strain ATCC 25196 / NCIMB 11849) [Complete proteome] [HAMAP]

Taxonomic identifier

323848 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Nitrosomonadales › Nitrosomonadaceae › Nitrosospira › Nitrosospira multiformis

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis By similarity. HAMAP-Rule MF_00118
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Elongation factor
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: HAMAP GTPase activity Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 396	396	Elongation factor Tu HAMAP-Rule MF_00118		PRO_0000337447
Regions
	Nucleotide binding	19 – 26	8	GTP By similarity
	Nucleotide binding	81 – 85	5	GTP By similarity
	Nucleotide binding	136 – 139	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2YAZ9 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 382644477F2FCC00	FASTA	396	42,925
	10 20 30 40 50 60 MAKSKFERTK PHINVGTIGH VDHGKTTLTA AITMVLAKKF GGEAKSYAQI DSAPEEKARG 70 80 90 100 110 120 ITINTSHVEY ETEKRHYAHV DCPGHADYVK NMITGAAQMD GAILVVSAAD GPMPQTREHI 130 140 150 160 170 180 LLARQVGVPY IIVYMNKADM VDDAELLELV EMEVRELLSK YNFPGDDTPI VIGSALKALE 190 200 210 220 230 240 GDQSDIGEPS IYKLAAALDS YIPEPQRAVD GAFLMPVEDV FSISGRGTVV TGRVERGVIK 250 260 270 280 290 300 VGEDIEIVGL KPTTKTVCTG VEMFRKLLDQ GQAGDNVGVL LRGTKREEVE RGQVLAKPGT 310 320 330 340 350 360 ITPHTKFTAE IYVLSKEEGG RHTPFFQGYR PQFYFRTTDV TGAIELPAGT EMVMPGDNVS 370 380 390 VTVNLIAPIA MEEGLRFAIR EGGRTVGAGV VAKIIE

References

[1]

"Complete sequence of chromosome 1 of Nitrosospira multiformis ATCC 25196." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25196 / NCIMB 11849.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000103 Genomic DNA. Translation: ABB74059.1. CP000103 Genomic DNA. Translation: ABB74072.1.
RefSeq	YP_411451.1. NC_007614.1. YP_411464.1. NC_007614.1.
3D structure databases
HSSP	HSSP built from PDB template 2BVN based on UniProtKB P0A6N1.
ProteinModelPortal	Q2YAZ9.
SMR	Q2YAZ9. Positions 2-395.
ModBase	Search...
Protein-protein interaction databases
STRING	323848.Nmul_A0765.
Proteomic databases
PRIDE	Q2YAZ9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABB74059; ABB74059; Nmul_A0752. ABB74072; ABB74072; Nmul_A0765.
GeneID	3785218. 3786487.
KEGG	nmu:Nmul_A0752. nmu:Nmul_A0765.
PATRIC	22724596. VBINitMul110821_0870.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0050.
HOGENOM	HOG000229290.
KO	K02358.
OMA	CEFVGYN.
ProtClustDB	PRK00049.
Family and domain databases
HAMAP	MF_00118_B. EF_Tu_B.
InterPro	IPR000795. EF_GTP-bd_dom. IPR005225. Small_GTP-bd_dom. IPR009001. Transl_elong_EF1A/Init_IF2_C. IPR004161. Transl_elong_EFTu/EF1A_2. IPR004541. Transl_elong_EFTu/EF1A_bac/org. IPR004160. Transl_elong_EFTu/EF1A_C. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Pfam	PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF03143. GTP_EFTU_D3. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SUPFAM	SSF50465. Elong_init_C. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00485. EF-Tu. 1 hit. TIGR00231. small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFTU_NITMU

Accession

Primary (citable) accession number: Q2YAZ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	December 20, 2005
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program