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Protein

Malate dehydrogenase

Gene

mdh

Organism
Nitrosospira multiformis (strain ATCC 25196 / NCIMB 11849)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei92 – 921SubstrateUniRule annotation
Binding sitei98 – 981SubstrateUniRule annotation
Binding sitei105 – 1051NADUniRule annotation
Binding sitei112 – 1121NADUniRule annotation
Binding sitei131 – 1311SubstrateUniRule annotation
Binding sitei162 – 1621SubstrateUniRule annotation
Active sitei187 – 1871Proton acceptorUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 177NADUniRule annotation
Nucleotide bindingi129 – 1313NADUniRule annotation

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. malate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciNMUL323848:GKEC-885-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:Nmul_A0864
OrganismiNitrosospira multiformis (strain ATCC 25196 / NCIMB 11849)
Taxonomic identifieri323848 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosospira
ProteomesiUP000002718: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 327327Malate dehydrogenasePRO_0000294396Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi323848.Nmul_A0864.

Structurei

3D structure databases

ProteinModelPortaliQ2YAQ4.
SMRiQ2YAQ4. Positions 1-325.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000220953.
KOiK00024.
OMAiHGEQMAV.
OrthoDBiEOG6PP9Q2.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2YAQ4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKTPIRVAVT GAAGQIAYSL LFRIAAGDML GEDQPVILQL LDIPQSLPSL
60 70 80 90 100
KGVVMELDDC AFPLLRDITI TDDPKTAFRD INIAMLVGAR PRTKGMERKD
110 120 130 140 150
LLEANGTIFR AQGKALDEVA GRDVKVLVVG NPANTNAYIT MKNAPSLKPT
160 170 180 190 200
SFSSMMRLDH NRAVFQLAVK VGQPVSSVRK MIVWGNHSSA QYPDLSHAEV
210 220 230 240 250
DGHNAADLVN DMAWIETGFI PVIQKRGMEV IEARGSSSAA SAANAAICHM
260 270 280 290 300
RDWVSGTPEG DWVSMGIPSD GSYGIPEGVI YGYPVTCQGG EYKIVPDLEI
310 320
SEFSRMKMQA SYRELMGERE SIKHLLG
Length:327
Mass (Da):35,521
Last modified:December 20, 2005 - v1
Checksum:iC0389CE276C78698
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000103 Genomic DNA. Translation: ABB74167.1.
RefSeqiYP_411559.1. NC_007614.1.

Genome annotation databases

EnsemblBacteriaiABB74167; ABB74167; Nmul_A0864.
GeneIDi3784434.
KEGGinmu:Nmul_A0864.
PATRICi22724872. VBINitMul110821_1007.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000103 Genomic DNA. Translation: ABB74167.1.
RefSeqiYP_411559.1. NC_007614.1.

3D structure databases

ProteinModelPortaliQ2YAQ4.
SMRiQ2YAQ4. Positions 1-325.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi323848.Nmul_A0864.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABB74167; ABB74167; Nmul_A0864.
GeneIDi3784434.
KEGGinmu:Nmul_A0864.
PATRICi22724872. VBINitMul110821_1007.

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000220953.
KOiK00024.
OMAiHGEQMAV.
OrthoDBiEOG6PP9Q2.

Enzyme and pathway databases

BioCyciNMUL323848:GKEC-885-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome 1 of Nitrosospira multiformis ATCC 25196."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Richardson P.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25196 / NCIMB 11849.

Entry informationi

Entry nameiMDH_NITMU
AccessioniPrimary (citable) accession number: Q2YAQ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: December 20, 2005
Last modified: February 4, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.