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Q2Y9Y9 (BIOB_NITMU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Nmul_A1129
OrganismNitrosospira multiformis (strain ATCC 25196 / NCIMB 11849) [Complete proteome] [HAMAP]
Taxonomic identifier323848 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosospira

Protein attributes

Sequence length342 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 342342Biotin synthase HAMAP-Rule MF_01694
PRO_0000381504

Sites

Metal binding821Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding861Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding891Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1261Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1571Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2171Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2891Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2Y9Y9 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: F1F23EA64ADA84F7

FASTA34237,172
        10         20         30         40         50         60 
MSQSASGIVN IETSSGGAVD SFADKLSGIS ADFRRWDVRR VEALLDLPFS DLIHRAQLVH 

        70         80         90        100        110        120 
RQYHDPNGVQ LSTLISVKTG GCPEDCGYCP QAARYHTNVE NQDMLTVEDV IAAAAAAKAQ 

       130        140        150        160        170        180 
GATRFCMGAA WRGPKERDMK SVVEMVRAVK ALGLEACTTL GMLKPGQAEQ LQEAGLDYYN 

       190        200        210        220        230        240 
HNLDTSPEFY GEIITTRDYE DRLDTLQKVR HAGINVCCGG IVGMGESRRA RAGLIAQLAN 

       250        260        270        280        290        300 
LDPYPESVPI NHLVQVEGTP LHGTEALDPL EFVRTIAAAR ITMPKAMVRL SAGRREMPDA 

       310        320        330        340 
VQALCFLAGA NSIFYGDKLL TTGNPEAERD KALFDRLGLH SI 

« Hide

References

[1]"Complete sequence of chromosome 1 of Nitrosospira multiformis ATCC 25196."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Richardson P.
Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25196 / NCIMB 11849.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000103 Genomic DNA. Translation: ABB74432.1.
RefSeqYP_411824.1. NC_007614.1.

3D structure databases

ProteinModelPortalQ2Y9Y9.
SMRQ2Y9Y9. Positions 35-340.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING323848.Nmul_A1129.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABB74432; ABB74432; Nmul_A1129.
GeneID3784242.
KEGGnmu:Nmul_A1129.
PATRIC22725486. VBINitMul110821_1311.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMAADRFCMG.
OrthoDBEOG622PMP.
ProtClustDBCLSK2298556.

Enzyme and pathway databases

BioCycNMUL323848:GKEC-1153-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_NITMU
AccessionPrimary (citable) accession number: Q2Y9Y9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: December 20, 2005
Last modified: February 19, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways