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Q2Y9Y9

- BIOB_NITMU

UniProt

Q2Y9Y9 - BIOB_NITMU

Protein

Biotin synthase

Gene

bioB

Organism
Nitrosospira multiformis (strain ATCC 25196 / NCIMB 11849)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (20 Dec 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi82 – 821Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi86 – 861Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi89 – 891Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi126 – 1261Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi157 – 1571Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi217 – 2171Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi289 – 2891Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciNMUL323848:GKEC-1153-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:Nmul_A1129
    OrganismiNitrosospira multiformis (strain ATCC 25196 / NCIMB 11849)
    Taxonomic identifieri323848 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosospira
    ProteomesiUP000002718: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 342342Biotin synthasePRO_0000381504Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi323848.Nmul_A1129.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2Y9Y9.
    SMRiQ2Y9Y9. Positions 35-340.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239957.
    KOiK01012.
    OMAiRIMMPAS.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q2Y9Y9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQSASGIVN IETSSGGAVD SFADKLSGIS ADFRRWDVRR VEALLDLPFS    50
    DLIHRAQLVH RQYHDPNGVQ LSTLISVKTG GCPEDCGYCP QAARYHTNVE 100
    NQDMLTVEDV IAAAAAAKAQ GATRFCMGAA WRGPKERDMK SVVEMVRAVK 150
    ALGLEACTTL GMLKPGQAEQ LQEAGLDYYN HNLDTSPEFY GEIITTRDYE 200
    DRLDTLQKVR HAGINVCCGG IVGMGESRRA RAGLIAQLAN LDPYPESVPI 250
    NHLVQVEGTP LHGTEALDPL EFVRTIAAAR ITMPKAMVRL SAGRREMPDA 300
    VQALCFLAGA NSIFYGDKLL TTGNPEAERD KALFDRLGLH SI 342
    Length:342
    Mass (Da):37,172
    Last modified:December 20, 2005 - v1
    Checksum:iF1F23EA64ADA84F7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000103 Genomic DNA. Translation: ABB74432.1.
    RefSeqiYP_411824.1. NC_007614.1.

    Genome annotation databases

    EnsemblBacteriaiABB74432; ABB74432; Nmul_A1129.
    GeneIDi3784242.
    KEGGinmu:Nmul_A1129.
    PATRICi22725486. VBINitMul110821_1311.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000103 Genomic DNA. Translation: ABB74432.1 .
    RefSeqi YP_411824.1. NC_007614.1.

    3D structure databases

    ProteinModelPortali Q2Y9Y9.
    SMRi Q2Y9Y9. Positions 35-340.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 323848.Nmul_A1129.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABB74432 ; ABB74432 ; Nmul_A1129 .
    GeneIDi 3784242.
    KEGGi nmu:Nmul_A1129.
    PATRICi 22725486. VBINitMul110821_1311.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239957.
    KOi K01012.
    OMAi RIMMPAS.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci NMUL323848:GKEC-1153-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of chromosome 1 of Nitrosospira multiformis ATCC 25196."
      Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Richardson P.
      Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 25196 / NCIMB 11849.

    Entry informationi

    Entry nameiBIOB_NITMU
    AccessioniPrimary (citable) accession number: Q2Y9Y9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: December 20, 2005
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3