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Protein

Pimeloyl-[acyl-carrier protein] methyl ester esterase

Gene

bioH

Organism
Nitrosospira multiformis (strain ATCC 25196 / NCIMB 11849)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters.UniRule annotation

Catalytic activityi

Pimeloyl-[acyl-carrier protein] methyl ester + H2O = pimeloyl-[acyl-carrier protein] + methanol.UniRule annotation

Pathwayi: biotin biosynthesis

This protein is involved in the pathway biotin biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei23 – 231Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation
Active sitei90 – 901NucleophileUniRule annotation
Active sitei216 – 2161UniRule annotation
Active sitei244 – 2441UniRule annotation
Binding sitei244 – 2441SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Keywords - Biological processi

Biotin biosynthesis

Enzyme and pathway databases

BioCyciNMUL323848:GKEC-1155-MONOMER.
UniPathwayiUPA00078.

Protein family/group databases

ESTHERinitmu-q2y9y7. BioH.

Names & Taxonomyi

Protein namesi
Recommended name:
Pimeloyl-[acyl-carrier protein] methyl ester esteraseUniRule annotation (EC:3.1.1.85UniRule annotation)
Alternative name(s):
Biotin synthesis protein BioHUniRule annotation
Carboxylesterase BioHUniRule annotation
Gene namesi
Name:bioHUniRule annotation
Ordered Locus Names:Nmul_A1131
OrganismiNitrosospira multiformis (strain ATCC 25196 / NCIMB 11849)
Taxonomic identifieri323848 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosospira
Proteomesi
  • UP000002718 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 263263Pimeloyl-[acyl-carrier protein] methyl ester esterasePRO_1000067273Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi323848.Nmul_A1131.

Structurei

3D structure databases

ProteinModelPortaliQ2Y9Y7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni90 – 912Substrate bindingUniRule annotation
Regioni152 – 1565Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D3C. Bacteria.
COG0596. LUCA.
HOGENOMiHOG000028062.
KOiK02170.
OMAiICPLEAG.
OrthoDBiEOG6FJNH9.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01260. Carboxylester.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR010076. BioH.
IPR000639. Epox_hydrolase-like.
[Graphical view]
PfamiPF12697. Abhydrolase_6. 1 hit.
[Graphical view]
PRINTSiPR00111. ABHYDROLASE.
PR00412. EPOXHYDRLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR01738. bioH. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2Y9Y7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQVSLYVES LGEGPDLVLL HGWAMHSGMW GSTRRSLAQH FRLHLVDLPG
60 70 80 90 100
HGFSRGALPY KRGEKNGVSE DMVERVVEVL PPDCVICGWS LGGQLAIELA
110 120 130 140 150
LREPARVEKI ILTSTTPSFV KREDWQWGME ELTLKAFAEN LRRDFSTTMK
160 170 180 190 200
RFLTLQVSGG GDAGKVLPEM RRLLFERSAP EPEALEAGLQ IVLANDLRGK
210 220 230 240 250
LRNIVQPTLL IHGENDVIAH PEAAAWMKQQ FQDVELAMLP NCSHVPFLSY
260
PDKFIANIVR FAR
Length:263
Mass (Da):29,449
Last modified:December 20, 2005 - v1
Checksum:i884BC7C4355682A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000103 Genomic DNA. Translation: ABB74434.1.
RefSeqiWP_011380475.1. NC_007614.1.

Genome annotation databases

EnsemblBacteriaiABB74434; ABB74434; Nmul_A1131.
KEGGinmu:Nmul_A1131.
PATRICi22725490. VBINitMul110821_1313.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000103 Genomic DNA. Translation: ABB74434.1.
RefSeqiWP_011380475.1. NC_007614.1.

3D structure databases

ProteinModelPortaliQ2Y9Y7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi323848.Nmul_A1131.

Protein family/group databases

ESTHERinitmu-q2y9y7. BioH.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABB74434; ABB74434; Nmul_A1131.
KEGGinmu:Nmul_A1131.
PATRICi22725490. VBINitMul110821_1313.

Phylogenomic databases

eggNOGiENOG4105D3C. Bacteria.
COG0596. LUCA.
HOGENOMiHOG000028062.
KOiK02170.
OMAiICPLEAG.
OrthoDBiEOG6FJNH9.

Enzyme and pathway databases

UniPathwayiUPA00078.
BioCyciNMUL323848:GKEC-1155-MONOMER.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01260. Carboxylester.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR010076. BioH.
IPR000639. Epox_hydrolase-like.
[Graphical view]
PfamiPF12697. Abhydrolase_6. 1 hit.
[Graphical view]
PRINTSiPR00111. ABHYDROLASE.
PR00412. EPOXHYDRLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR01738. bioH. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome 1 of Nitrosospira multiformis ATCC 25196."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Richardson P.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25196 / NCIMB 11849.

Entry informationi

Entry nameiBIOH_NITMU
AccessioniPrimary (citable) accession number: Q2Y9Y7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: December 20, 2005
Last modified: November 11, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.