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Protein

Pimeloyl-[acyl-carrier protein] methyl ester esterase

Gene

bioH

Organism
Nitrosospira multiformis (strain ATCC 25196 / NCIMB 11849 / C 71)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters.UniRule annotation

Catalytic activityi

Pimeloyl-[acyl-carrier protein] methyl ester + H2O = pimeloyl-[acyl-carrier protein] + methanol.UniRule annotation

Pathwayi: biotin biosynthesis

This protein is involved in the pathway biotin biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei23Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation1
Active sitei90NucleophileUniRule annotation1
Active sitei216UniRule annotation1
Active sitei244UniRule annotation1
Binding sitei244SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Serine esterase
Biological processBiotin biosynthesis

Enzyme and pathway databases

BioCyciNMUL323848:G1GTI-1187-MONOMER
UniPathwayiUPA00078

Protein family/group databases

ESTHERinitmu-q2y9y7 BioH

Names & Taxonomyi

Protein namesi
Recommended name:
Pimeloyl-[acyl-carrier protein] methyl ester esteraseUniRule annotation (EC:3.1.1.85UniRule annotation)
Alternative name(s):
Biotin synthesis protein BioHUniRule annotation
Carboxylesterase BioHUniRule annotation
Gene namesi
Name:bioHUniRule annotation
Ordered Locus Names:Nmul_A1131
OrganismiNitrosospira multiformis (strain ATCC 25196 / NCIMB 11849 / C 71)
Taxonomic identifieri323848 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNitrosomonadalesNitrosomonadaceaeNitrosospira
Proteomesi
  • UP000002718 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000672731 – 263Pimeloyl-[acyl-carrier protein] methyl ester esteraseAdd BLAST263

Proteomic databases

PRIDEiQ2Y9Y7

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi323848.Nmul_A1131

Structurei

3D structure databases

ProteinModelPortaliQ2Y9Y7
SMRiQ2Y9Y7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni90 – 91Substrate bindingUniRule annotation2
Regioni152 – 156Substrate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D3C Bacteria
COG0596 LUCA
HOGENOMiHOG000028062
KOiK02170
OMAiLHGWGMN
OrthoDBiPOG091H05R7

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
HAMAPiMF_01260 Carboxylester, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR000073 AB_hydrolase_1
IPR010076 BioH
IPR000639 Epox_hydrolase-like
PfamiView protein in Pfam
PF12697 Abhydrolase_6, 1 hit
PRINTSiPR00111 ABHYDROLASE
PR00412 EPOXHYDRLASE
SUPFAMiSSF53474 SSF53474, 1 hit
TIGRFAMsiTIGR01738 bioH, 1 hit

Sequencei

Sequence statusi: Complete.

Q2Y9Y7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQVSLYVES LGEGPDLVLL HGWAMHSGMW GSTRRSLAQH FRLHLVDLPG
60 70 80 90 100
HGFSRGALPY KRGEKNGVSE DMVERVVEVL PPDCVICGWS LGGQLAIELA
110 120 130 140 150
LREPARVEKI ILTSTTPSFV KREDWQWGME ELTLKAFAEN LRRDFSTTMK
160 170 180 190 200
RFLTLQVSGG GDAGKVLPEM RRLLFERSAP EPEALEAGLQ IVLANDLRGK
210 220 230 240 250
LRNIVQPTLL IHGENDVIAH PEAAAWMKQQ FQDVELAMLP NCSHVPFLSY
260
PDKFIANIVR FAR
Length:263
Mass (Da):29,449
Last modified:December 20, 2005 - v1
Checksum:i884BC7C4355682A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000103 Genomic DNA Translation: ABB74434.1
RefSeqiWP_011380475.1, NZ_FNVK01000008.1

Genome annotation databases

EnsemblBacteriaiABB74434; ABB74434; Nmul_A1131
KEGGinmu:Nmul_A1131

Similar proteinsi

Entry informationi

Entry nameiBIOH_NITMU
AccessioniPrimary (citable) accession number: Q2Y9Y7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: December 20, 2005
Last modified: May 23, 2018
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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