Q2Y9Y5 (BIOD_NITMU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3

Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase

Gene names

Name:	bioD
Ordered Locus Names:	Nmul_A1133

Organism

Nitrosospira multiformis (strain ATCC 25196 / NCIMB 11849) [Complete proteome] [HAMAP]

Taxonomic identifier

323848 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Nitrosomonadales › Nitrosomonadaceae › Nitrosospira

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336
Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Subcellular location	Cytoplasm By similarity HAMAP MF_00336.
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dethiobiotin synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 226	226	ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336		PRO_0000302537
Regions
	Nucleotide binding	114 – 117	4	ATP By similarity
	Nucleotide binding	174 – 175	2	ATP By similarity
Sites
	Metal binding	19	1	Magnesium 2 By similarity
	Metal binding	53	1	Magnesium 2 By similarity
	Metal binding	114	1	Magnesium 2 By similarity
	Binding site	53	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2Y9Y5 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 3990DCBE8153510A	FASTA	226	23,960
	10 20 30 40 50 60 MGMGKGYFVT GTDTGVGKTR VTCALLHAFA ATGKTVVGMK PVAAGCENGM WPDVELLAAA 70 80 90 100 110 120 SNISVQREHI NPYALVPPIA PHIAADRAGI EIDLEVIRQA HLELKKKADI VIVEGAGGFL 130 140 150 160 170 180 VPLNDHEDSV ALVQALGLAV LLVVGMRLGC INHALLTAHA VRAAQIPLAG WVANRIDPEM 190 200 210 220 AVFKENVLAL EQRLDCPLLG ILPYDQNHDA RDLSSLLDIA RIGMSS

References

[1]

"Complete sequence of chromosome 1 of Nitrosospira multiformis ATCC 25196." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25196 / NCIMB 11849.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000103 Genomic DNA. Translation: ABB74436.1.
RefSeq	YP_411828.1. NC_007614.1.
3D structure databases
ProteinModelPortal	Q2Y9Y5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2Y9Y5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3784246.
GenomeReviews	Gene locus Nmul_A1133 in contig CP000103_GR.
KEGG	nmu:Nmul_A1133.
PATRIC	22725494. VBINitMul110821_1315.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0132.
HOGENOM	HBG650065.
OMA	INPGLSH.
PhylomeDB	Q2Y9Y5.
ProtClustDB	CLSK586185.
Enzyme and pathway databases
BioCyc	NMUL323848:NMUL_A1133-MONOMER.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR002586. CbiA_P_synth. IPR004472. DTB_synth_BioD. [Graphical view]
KO	K01935.
Pfam	PF01656. CbiA. 1 hit. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. BioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_NITMU

Accession

Primary (citable) accession number: Q2Y9Y5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	December 20, 2005
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program